菌株<i>Cupriavidus</i> sp. DT-1对液体和土壤中TCP的降解

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Abstract Cupriavidus sp. DT-1 was a 3,5,6-trichloro-2-pyridinol (TCP)-degrading strain which could transform TCP to 2-hydroxypyridine (2-HP). Liquid-mass spectrometry (HPLC-MS) was used to detect the degradation products of 2-HP, And the methods of triparetal conjugation, quantitative real-time PCR (q-PCR) were used to evaluate the remediation effect of TCP-contaminated soils by the degrading-bacterium. Results showed that strain DT-1 was able to further degrade 2-HP, and sequentially produced nicotine blue, maleamic acid and fumaric acid, until it was transformed into the carbon source that could support the growth of strain DT-1. Pilot experiment showed that inoculation of strain DT-1 remarkably accelerated the elimination of TCP in soils. The degradation rates of TCP in inoculated soils were 94.4% and 86.7%, while those in uninoculated soils were 20.4% and 28.4%, respectively. Green fluorescent protein encoding gene gfp harbored strain DT-1-gfp could survive in soils for more than 35d. The results of q-PCR showed that inoculation of strain DT-1-gfp significantly improved the recovery of bacterial community abundance in the TCP-contaminated soils.

Key words： Cupriavidus sp. DT-1 3,5,6-trichloro-2-pyridinol (TCP) 2-hydroxypyridine (2-HP) bioremediation

Received: 28 October 2020

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X172

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	LU Peng
	ZHOU Hui
	YUAN Meng

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LU Peng,ZHOU Hui,YUAN Meng. Biodegradation of 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1 in liquid and soil environments[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(6): 2780-2787.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I6/2780

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