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| Study on the distribution and removal effect of resistance genes in integrated system of A2/O-MBR |
| LI Zhong-hong1,2, DU Cai-li2,3, CHEN Su-hua1, ZHANG Lie-yu2, LI Xiao-guang2, LI Jia-xi2, TIAN Zhen-jun2 |
1. Key Laboratory of Jiangxi Province for Persistant Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang 330063, China;
2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
3. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China |
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Abstract The increase of antibiotic resistance genes among microorganisms has become the main transmission source for sewage treatment plants. The purpose of this study was to explore the removal effect of Antibiotic Resistance Genes (ARGs) and pathogenic bacteria in rural domestic sewage treatment process and evaluate the water quality safety. This study selected a integration A2/O-MBR wastewater treatment system in the village of Beijing, systematic study was implemented in integrated system of A2/O-MBR wastewater treatment system to get the distribution law of ARGs and pathogenic bacteria in the each processing unit. Based on macro genomics, the high-throughput sequencing technology was used to analysis the the ability of removing ARGs and pathogenic bacteria in genus through integrated system of A2/O-MBR. Results showed that the ARGs widely existed in sewage treatment system, 19kinds of ARGs including tetracycline class, aminoglycoside class and sulfonamide class were detected, the relative abundance of the ARGs in influent was much higher than its concentration in the effluent, relative abundance of the ARGs fell by 72.25%, but most of the ARGs in sewage treatment system was not fully removed. The changes of microbial community structure showed that the relative abundance of 32potential pathogens decreased significantly, and most pathogenic bacteria could not be completely removed. The residual ARGs and pathogenic bacteria in the water will lead to the potential pollution risk of receiving water body.
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Received: 25 February 2021
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