一种浮游植物快速浓缩及固定方法的可靠性分析

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Abstract In order to verify the reliability of a membrane concentration method to rapidly concentrate and fixe phytoplankton in the field, the "Lugol's solution fixed sedimentation method (LSM)" and "membrane concentration method (MCM)" were comparatively used here to authenticate different phytoplankton samples. The results show that:(1) 7 phyla and 32 genera were both identified by the two method, but using the MCM could authenticate more phytoplankton species than the LSM method; (2) There was no significant difference not only in phytoplankton abundance between the two methods except Euglenophyta (P<0.05) at the phylum level (P>0.05), but also in phytoplankton relative abundance within 99% at the genus level (P>0.05). Which meant that, at the phylum and genus levels, the MCM was feasible to identify the phytoplankton abundance; (3) There was no significant difference in the slope of fitting equation of different algae biomass gradients (P>0.05), and they showed a high correlation coefficient (R²=0.958, P<0.01), however, algal biomass of the MCM seemed to be a little larger. On a conclusion, the results of the MCM was consistent with the LSM, and the MCM had more advantages in less environmental condition requirement, easier for field monitoring, and save time.

Key words： Lugol's solution fixed sedimentation method membrane concentration method phytoplankton species species abundance linear fitting

Received: 22 January 2021

PACS:	X52
	X171.5

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	Articles by authors
	LI Ya-li
	YANG Zheng-jian
	TIAN Pan
	LIU Yi
	WANG Cong-feng
	JI Dao-bin
	LIU De-fu

Cite this article:

LI Ya-li,YANG Zheng-jian,TIAN Pan等. Reliability analysis of a method to rapidly concentrate and fix phytoplankton[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4300-4309.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I9/4300

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