陕北石油污染土壤微生物种群变化及影响因素

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Abstract Oil-contaminated soil and uncontaminated soil from oil well sites in ten oil-producing counties in Northern Shaanxi were collected. Chemical analysis and molecular biology techniques were used to determine the physicochemical properties and bacterial community structure of soil. Methods such as Pearson correlation analysis and redundancy analysis were used to study the impact of oil pollution on physicochemical properties, microbial population distribution and diversity of soil. Results showed that compared with uncontaminated soil, nitrate-nitrogen, total organic carbon, total carbon, total sulfur and carbon-nitrogen ratio of oil-contaminated soil increased, while the pH value decreased. The richness of soil bacterial community did not change significantly, but the evenness decreased significantly. The relative abundance of some dominant bacterial changed obviously after oil contamination in different regions. Changes in relative abundance of Proteobacteria and Chloroflexi were significantly positively correlated with petroleum hydrocarbon content, and changes in relative abundance of Candidatus Saccharibacteria were significantly negatively correlated with petroleum hydrocarbon content. The relative abundance of Pseudomonas, Nocardioides, Arthrobacter, Acinetobacter, Alkanindiges, Gaiella and GP4 increased significantly in Dingbian, Jingbian, Zichang, Suide, Wuqi, Ansai and Fuxian. The relative abundance of Ohtaekwangia, Pseudonocardia, Ramlibacter, and Sacchariacteria_genera_incertae_sedis decreased significantly in Dingbian, Jingbian, Zichang and Suide. Pseudomonas, Nocardioides, Arthrobacter and Alkanindiges were the main oilphilic bacteria in Northern Shaanxi Province. Physicochemical properties such as pH, moisture content, total petroleum hydrocarbon and total organic carbon were the key factors affecting the microbial population structure of oil-contaminated soil in Northern Shaanxi oilfield.

Key words： petroleum contaminated soil bacterial community structure physicochemical properties influencing factors regional characteristics

Received: 20 January 2021

PACS:	X172
	X53

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	XU Yin-rui
	WU Man-li
	WANG Li
	GAO Huan
	LIU Ze-liang

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XU Yin-rui,WU Man-li,WANG Li等. The influences of petroleum pollution on the microbial population distribution in Northern Shaanxi Province of China[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4349-4359.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I9/4349

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