广东省蛭态轮虫物种多样性及其系统发育研究

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Abstract In order to investigate species diversity of bdelloid rotifer in Guangdong Province, 98 samples from different habitats in Guangdong Province were collected, and 66 species (including subspecies) of bdelloid rotifers were recorded belonged to 3 orders, 4 families, 12 genera. Fifty-six species of bdelloid rotifer were identified in terrestrial environment, including 42 species in bryophyte habitats, 40 species in deciduous habitats, 12 species in lichen habitats, and 15species in aquatic habitats. The species number of bdelloid rotifer in terrestrial habitats was significantly more than that in aquatic habitats. To verify the accuracy of morphological identification, 37 COI gene sequences of 16 species belonged to 4genera were obtained, and the genetic distance among them ranged from 8.59% to 23.17%. The COI gene sequences of Adinet cuneata and Philodina rapida were first reported in the world. The total content (71.2%) of adenine (A) and thymine (T) was higher than that (28.8%) of guanine (G) and cytosine(C). The cluster results of phylogenetic tree were consistent with those of morphological species identification; the species with similar morphology belonged to the same genus have higher homology. The results showed that the combination of morphological identification and DNA barcoding technology could improve the accuracy of species identification for bdelloid rotifers, explore their phylogenetic position at molecular level, and promote the species diversity and ecological research of bdelloid rotifers.

Key words： bdelloid rotifer biodiversity DNA barcoding COI gene biogeography

Received: 18 February 2021

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	WANG Qing
	LI Ying
	WANG Wen-bo
	YANG Yu-feng

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WANG Qing,LI Ying,WANG Wen-bo等. Species diversity and phylogenetic analysis of bdelloid rotifers in Guangdong Province[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4367-4377.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I9/4367

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