污染场地土壤重金属的生物可给性及毒性研究

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Abstract Recently, bioaccessibility of metals has been applied for assessing the health risk of field soils, however the bioaccessibility of metals is varied and there are few studies on the toxic effects of bioaccessible metals on human body. In this study, fives soil samples (S1-S5) from an electronic disassembly plant in Wenling, Zhejiang Province were sampled. The total concentrations and bioaccessibility of Zn, Cu, Cd and Pb in the soils were determined. Moreover, the toxic effects of bioaccessible heavy metals on human intestinal epithelial cells were also evaluated. The results demonstrated that all samples were polluted by Cd, Pb, Zn, and Cu, with the greatest concentrations of Cd (4.84mg/kg) and Cu (438.52mg/kg). The bioaccessibility of the four heavy metals in the gastric and intestinal phase was 2.10%~48.28%, 4.84%~33.73%, 16.04%~42.81%, 1.81%~15.71%, and 2.05%~36.91%, 13.17%~22.23%, 10.19%~23.10%, 0.60%~2.69%, respectively. In general, the bioaccessibility of four heavy metals in the gastric phase was lower than that in the intestine phase. After exposure to intestinal bioaccessible extracts of different soils for 24h, cell viability significantly decreased except for sample S4. In addition, there was no significant change in SOD activity after treatment with S3 and S5samples, but CAT activity was decreased. Furthermore, DNA damage was trigged by those samples. To investigate the bioaccessibility and its toxic effects of fields soils, this study provides a scientific basis for the health risk assessment of soil in China.

Key words： electronic dismantling field soils bioaccessibility Caco-2 DNA damage

Received: 18 February 2021

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	Articles by authors
	MA Jiao-yang
	TIAN Wen
	WANG Kun
	BAO Xin-chen
	WANG Jie
	CUI Dao-lei
	XIANG Ping

Cite this article:

MA Jiao-yang,TIAN Wen,WANG Kun等. Bioaccessibility and their toxic effects of heavy metal in field soils from an electronic disassembly plant[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(10): 4885-4893.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I10/4885

[1]	Ohajinwa C M, van Bodegom P M, Vijver M G, et al. Impact of informal electronic waste recycling on metal concentrations in soils and dusts[J]. Environmental Research, 2018,164:385-394.
[2]	Okeme J O, Arrandale V H. Electronic waste recycling:Occupational exposures and work-related health effects[J]. Current Environmental Health Reports, 2019,6(4):256-268.
[3]	Akram R, Natasha, Fahad S, et al. Trends of electronic waste pollution and its impact on the global environment and ecosystem[J]. Environ Sci Pollut Res Int, 2019,26(17):16923-16938.
[4]	Qin G, Niu Z, Yu J, et al. Soil heavy metal pollution and food safety in China:effects, sources and removing technology[J]. Chemosphere, 2021,267:129205.
[5]	Boim A G F, Wragg J, Canniatti-Brazaca S G, et al. Human intestinal Caco-2cell line in vitro assay to evaluate the absorption of Cd, Cu, Mn and Zn from urban environmental matrices[J]. Environmental Geochemistry and Health, 2019,42(2):601-615.
[6]	冯康宏,范缙,Hii L U S,等.基于生物可给性的某冶炼厂土壤重金属健康风险评价[J]. 中国环境科学, 2021,41(1):442-450.Feng K H, Fan J, Hii L U S, et al. Human health risk assessment of heavy metals in soil from a smelting plant based on bioaccessibility[J]. China Environmental Science, 2021,41(1):442-450.
[7]	Li S W, Sun H J, Li H B, et al. Assessment of cadmium bioaccessibility to predict its bioavailability in contaminated soils[J]. Environment International, 2016,94:600-606.
[8]	Li H B, Li M-Y, Zhao D, et al. Arsenic, lead, and cadmium bioaccessibility in contaminated soils:Measurements and validations[J]. Critical Reviews in Environmental Science and Technology, 2020,50(13):1303-1338.
[9]	Amponsah L O, Dodd M, G D. Gastric bioaccessibility and human health risks associated with soil metal exposure via ingestion at an e-waste recycling site in Kumasi, Ghana[J]. Environmental Geochemistry and Health, 2020.
[10]	Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease[J]. Toxicology, 2011,283(2/3):65-87.
[11]	USEPA. Method 3050B:acid digestion of sediments, sludges, and soils[R]. Washington, DC:USEPA. 1996.
[12]	Yin N, Cai X, Du H, et al. In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2cells[J]. Chemosphere, 2017,168:358-364.
[13]	中国环境监测总站.中国土壤元素背景值[M]. 北京:中国环境科学出版社, 1990.China National Environmental Monitoring Centre. The soil environmental background value in the People's Public of China[M]. Beijing:China Environmental Science Press, 1990.
[14]	GB15618-2018土壤环境质量标准[S].GB15618-2018 Standard of soil environmental quality[S].
[15]	赵科理,傅伟军,叶正钱,等.电子垃圾拆解区土壤重金属空间异质性及分布特征[J]. 环境科学, 2016,37(8):3151-3159.Zhao K L, Fu W J, Ye Z Q, et al. Spatial variation of soil heavy metals in an e-waste dismantling area and their distribution characteristics[J]. Environmental Science, 2016,37(8):3151-3159.
[16]	陈晓晨,黄振佳,陈雨晴,等.基于in vitro试验的中国典型土壤中砷的健康风险及影响因素[J/OL]. 土壤学报, 2021.DOI:10.11766/trxb202005140100.Chen X C, Huang Z J, Chen Y Q, et al. In vitro test-based study on health risks of arsenic in typical soils of China and their influencing factors[J/OL]. Acta Pedologica Sinica, 2021. DOI:10.11766/trxb202005140100.
[17]	孙立强,孙崇玉,刘飞,等.淮北煤矿周边土壤重金属生物可给性及人体健康风险[J]. 环境化学, 2019,38(7):1453-1460.Sun L Q, Sun C Y, Liu F, et al. Bioaccessibility and health risk assessment of heavy metals in the soil around Huaibei coal mining area[J]. Environmental Chemistry, 2019,38(7):1453-1460.
[18]	Pelfrene A, Waterlot C, Mazzuca M, et al. Assessing Cd, Pb, Zn human bioaccessibility in smeltercontaminated agricultural topsoils (northern France)[J]. Environmental Geochemistry and Health, 2011,33(5):477-493.
[19]	Li Y, Padoan E, Ajmone-Marsan F. Soil particle size fraction and potentially toxic elements bioaccessibility:A review[J]. Ecotoxicol. Environ. Saf., 2021,209:111806.
[20]	Cao P, Fujimori T, Juhasz A, et al. Bioaccessibility and human health risk assessment of metal(loid)s in soil from an e-waste open burning site in Agbogbloshie, Accra, Ghana[J]. Chemosphere, 2020,240:124909.
[21]	Li H, Li J, Li S, et al. Application of oral bioavailability to remediation of contaminated soils:method development for bioaccessible As, Pb, and Cd[M]. Twenty Years of Research and Development on Soil Pollution and Remediation in China. 2018:189-216.
[22]	林承奇,蔡宇豪,胡恭任,等.闽西南土壤-水稻系统重金属生物可给性及健康风险[J]. 环境科学, 2021,42(01):359-367.Lin C Q, Cai Y H, Hu G R, et al. Bioaccessibility and health risks of the heavy metals in soil-rice system of southwest Fujian Province[J]. Environmental Science, 2021,42(01):359-367.
[23]	Saravanan S,耿华,谭小弟.肠道疾病中肠上皮细胞的死亡[J]. 生理学报, 2020,72(3):308-324.Saravanan S, Geng H, Tan X D. Cell death of intestinal epithelial cells in intestinal diseases[J]. Acta Physiologica Sinica, 2020,72(3):308-324.
[24]	Xiang P, He R W, Han Y-H, et al. Mechanisms of housedust-induced toxicity in primary human corneal epithelial cells:Oxidative stress, proinflammatory response and mitochondrial dysfunction[J]. Environment International, 2016,89-90:30-37.
[25]	Gao K, Zhang Y C, Niu J B, et al. Zinc promotes cell apoptosis via activating the Wnt-3a/beta-catenin signaling pathway in osteosarcoma[J]. Journal of Orthopaedic Surgery and Research, 2020,15(1):doi:10.1186/s13018-020-01585-x.
[26]	Wang Y H, Zhao W J, Zheng W J, et al. Effects of different zinc species on cellar zinc distribution, cell cycle, apoptosis and viability in MDAMB231cells[J]. Biological Trace Element Research, 2016,170(1):75-83.
[27]	Wang K, Ma J Y, Li M Y, et al. Mechanisms of Cd and Cu induced toxicity in human gastric epithelial cells:Oxidative stress, cell cycle arrest and apoptosis[J]. Science of the Total Environment, 2021,756:143951.
[28]	Santos S, Silva A M, Matos M, et al. Copper induced apoptosis in Caco-2 and Hep-G2 cells:Expression of caspases 3, 8 and 9, AIF and p53[J]. Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 2016,185-186:138-146.
[29]	Kim A, Park S, Sung J H. Cell viability and immune response to low concentrations of nickel and cadmium:An in vitro model[J]. International Journal of Environmental Research and Public Health, 2020,17(24):9218.
[30]	Xue C, Kang B, Su P, et al. MicroRNA-106b-5p participates in lead (Pb2+)-induced cell viability inhibition by targeting XIAP in HT-22and PC12cells[J]. Toxicology in Vitro, 2020,66:104876.
[31]	Husejnovic M S, Bergant M, Jankovic S, et al. Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2and HaCaT)[J]. Environmental Geochemistry and Health, 2018,40(4):1557-1572.
[32]	He X, Qi Z, Hou H, et al. Structural and functional alterations of gut microbiome in mice induced by chronic cadmium exposure[J]. Chemosphere, 2020,246:125747.
[33]	Maity S, Banerjee R, Goswami P, et al. Oxidative stress responses of two different ecophysiological species of earthworms (Eutyphoeus waltoni and Eisenia fetida) exposed to Cd-contaminated soil[J]. Chemosphere, 2018,203:307-317.
[34]	Aziz N, Butt A, Elsheikha H M. Antioxidant enzymes as biomarkers of Cu and Pb exposure in the ground spiders Lycosa terrestris and Pardosa birmanica[J]. Ecotoxicology and Environmental Safety, 2020, 190:110054.
[35]	Nojima Y, Ito K, Ono H, et al. Superoxide dismutases, SOD1and SOD2, play a distinct role in the fat body during pupation in silkworm Bombyx mori[J]. PLoS One, 2015,10(2):e0116007.
[36]	Souid G, Souayed N, Yaktiti F, et al. Effect of acute cadmium exposure on metal accumulation and oxidative stress biomarkers of Sparus aurata[J]. Ecotoxicology and Environmental Safety, 2013,89:1-7.
[37]	Atli G, Alptekin O, Tukel S, et al. Response of catalase activity to Ag+, Cd2+, Cr6+, Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromis niloticus[J]. Comparative Biochemistry and Physiology, 2006,143(2):218-224.
[38]	刘敏,赵苒.γH2AX检测在DNA双链断裂研究中应用[J]. 中国公共卫生, 2015,31(6):742-746.Liu M, Zhao R. Application of γH2AX assay in measurement of DNA double stand breaks[J]. Chinese Journal of Public Health, 2015,31(6):742-746.
[39]	王丽娜,罗志,张立.DNA损伤及其标志物γ-H2AX检测的研究进展[J]. 分析试验室, 2020,39(10):1131-1136.Wang L N, Luo Z, Zhang L. Advances in DNA damage and the determination methodologies of the marker γ-H2AX[J]. Chinese Journal of Analysis Laboratory, 2020,39(10):1131-1136.
[40]	Plappert-Helbig U, Libertini S, Frieauff W, et al. Gamma-H2AX immunofluorescence for the detection of tissue-specific genotoxicity in vivo[J]. Environmental and Molecular Mutagenesis, 2019,60(1):4-16.
[41]	Durrani K, El Din S A, Sun Y, et al. Ethyl maltol enhances copper mediated cytotoxicity in lung epithelial cells[J]. Toxicology and Applied Pharmacology, 2021,410:115354.
[42]	Villatoro-Pulido M, Font R, De Haro-Bravo M I, et al. Modulation of genotoxicity and cytotoxicity by radish grown in metal-contaminated soils[J]. Mutagenesis, 2009,24(1):51-57.