基于DGAOs富集的内碳源短程硝化反硝化工艺特性

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Abstract The operation performance of short-cut nitrification and denitrification process driven by internal carbon source at low C/N ratio was studied using Sequencing Batch Reactor (SBR) and anaerobic-aerobic-anoxia operation mode. The results showed that denitrifying golyphosphate accumulating Organisms (DGAOs) and ammonia-oxidizing Bacteria (AOB) can be enriched simultaneously in the reactor. DGAOs can use poly-β-hydroxyalkanoate (PHA) as internal carbon source for denitrification, and PHB (poly-β-hydroxy butyrate) was the main part of PHA. After stable operation, intracellular substances stored in sludge at the end of anaerobic phase could be clearly seen under fluorescence microscope at the 39th day, and 2.34mmol C/L in PHA stored by internal carbon source was consumed, which was 29% higher than the value reported in literature. After 55 days of domestication, the SBR system achieved a relatively stable denitrification effect. The average ammonia nitrogen removal rate was 93.13%±4.91%, and the denitrification efficiency of internal carbon source was 49.62%±8.97%. After domestication, denitrifying phosphate accumulating organisms(DPAOs) and nitrite oxidizing bacteria(AOB) were eliminated, denitrifying glycan bacteria and ammonia-oxidizing bacteria were enriched, and their abundance increased from 0.13% and 0.20% at inoculation to 7.13% and 1.11%, respectively, realizing the short-cut nitrification and denitrification driven by internal carbon source at low C/N.

Key words： denitrifying golyphosphate accumulating orgnisms ammonia oxidizing bacteria SBR short-cut nitrification internal carbon source denitrification

Received: 08 April 2021

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X703.1

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	ZHOU Qian
	ZHANG Lin
	TANG Xi
	TANG Chong-jian

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ZHOU Qian,ZHANG Lin,TANG Xi等. Short-cut nitrification and denitrification process characteristics of internal carbon source based on DGAOs enrichment[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5673-5679.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I12/5673

[1]	Sun S P, Nàcher C P, Merkey B, et al. Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N ratios: a review[J]. Environmental Engineering Science, 2010,27(2): 111-126.
[2]	Wu C Y. Optimization of biological nitrogen and phosphorus removal in A2/O wastewater treatment processes[M]. Nova Science Publishers, Incorporated, 2021.
[3]	Claus G, Kutzner H J. Denitrification of nitrate and nitric acid with methanol as carbon source[J]. Applied Microbiology & Biotechnology, 1985,22(5):378-381.
[4]	Her J J, Huang J S. Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough[J]. Bioresource Technology, 1995,54(1):45-51.
[5]	Jiang L, Liu J Y, Zuo K, et al. Performance of layered double hydroxides intercalated with acetate as biodenitrification carbon source: The effects of metal ions and particle size[J]. Bioresource Technology, 2018,259:99-103.
[6]	Metcalf, Eddy, Burton F L, et al. Wastewater engineering: treatment and reuse[M]. McGraw Hill, 2003.
[7]	Wang X, Wang S, Xue T, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77: 191-200.
[8]	李方舟,张琼,彭永臻.低DO硝化耦合内碳源反硝化脱氮处理生活污水[J]. 中国环境科学, 2019,39(4):1525-1532. Li F Z, Zhang Q, Peng Y Z. Treatment of domestic wastewater by low DO nitrification coupled with endogenous denitrification system[J]. China Environmental Science, 2019,39(4):1525-1532.
[9]	Wang X X, Wang S Y, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR)[J]. Water Research, 2016,95:319-329.
[10]	罗珊,李新,钟铭,等.低碳氮比生活污水短程硝化反硝化的快速启动研究[J]. 环境科学与技术, 2020,43(7):28-34. Luo S, Li X, Zhong M, et al. Rapid start-up of short-cut nitrification and denitrification od domestic wastewater with low C/N ratio: an experimental study[J]. Environmental Science & Technology, 2020, 43(7):28-34.
[11]	Jubany I, Carrera J, Lafuente J, et al. Start-up of a nitrification system with automatic control to treat highly concentrated ammonium wastewater: Experimental results and modeling[J]. Chemical Engineering Journal, 2008,144(3):407-419.
[12]	呼晓明,陈英文,严伟峰,等.生物流化床短程硝化的快速启动及影响因素研究[J]. 环境科学与技术, 2012,35(7):16-20. Hu X M, Chen Y W, Yan W F, et al. Quick start-up and influencing factors of partial nitrification in biological fluidized bed reactors[J]. Environmental Science & Technology, 2012,35(7):16-20.
[13]	李柏林,杨丹丹,黄馨,等.基于DO和游离氨联合控制的短程硝化快速启动及稳定运行研究[J]. 环境污染与防治, 2018,40(11):1219- 1223. Li B L, Yang D D, Huang X, et al. Study on rapid start-up and stability of partial nitrification based on controlling DO and free ammonia[J]. Environmental Pollution & Control, 2018,40(11):1219-1223.
[14]	Cech J S, Hartman P. Glucose induced break down of enhanced biological phosphate removal[J]. Environmental Technology, 1990, 11(7):651-656.
[15]	马民,陈银广,顾国维.聚磷菌与聚糖菌竞争影响因素的研究进展[J]. 环境科学与技术, 2006,1:102-104. Ma M, Chen Y G, Gu G W. Advances on the factors influencing competition between phosphorus-accumulating organisms and glycogen-accumulating organisms[J]. Environmental Science & Technology, 2006,1:102-104.
[16]	Whang L M, Filipe C, Park J K. Model-based evaluation of competition between polyphosphate-and glycogen-accumulating organisms[J]. Water Research, 2007,41(6):1312-1324.
[17]	刘小芳,郭海燕,张胜男,等.聚糖菌反硝化影响因素及内碳源转化特性[J]. 化工学报, 2019,70(3):1127-1134. Liu X F, Guo H Y, Zhang S N, et al. Influencing factors of denitrification of glycans and transformation characteristics of internal carbon sources[J]. CIESC Journal, 2019,70(3):1127-1134.
[18]	Tayà C, Garlapati V K, Guisasola A, et al. The selective role of nitrite in the PAO/GAO competition[J]. Chemosphere, 2013,93(4):612-618.
[19]	Laanbroek H J, Bodelier P L E, Gerards S. Oxygen consumption kinetics of Nitrosomonas europaea and Nitrobacter hamburgensis in mixed continuous cultures at different oxygen concentrations[J]. Archives of Microbiology, 1994,161(2):156-162.
[20]	Garrido J M, Van Benthurn W A J, Van Loosdrecht MCM, et al. Influence of dissolved oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor[J]. Biotechnology and Bioengineering, 1997,53:168-178.
[21]	Eaton A D, Clesceri L S, Greenberg A E, et al. Standard methods for the examination of water and wastewater[J]. American Journal of Public Health &Nations Health, 1966,56(3):387-388.
[22]	HJ/T 399-2007 水质化学需氧量的测定快速消解分光光度法[S]. HJ/T 399-2007 Water quality-Determination of the chemical oxygen demand - Fast digestion-spectrophotometric method[S].
[23]	李素芹,张军,王俊凤,等.磷钼蓝光度法测定磷酸钙中的磷含量[J]. 无机盐工业, 2008,3:55-56. Li S Q, Zhang J, Wang J F, et al. Determination of phosphorus content in calcium phosphate by phosphomolybdate blue spectrophotometry[J]. Inorganic Chemicals Industry, 2008,3:55-56.
[24]	Wang D B, Li X M, Yang Q, et al. Biological phosphorus removal in sequencing batch reactor with single-stage oxic process[J]. Bioresource Technology, 2008,99(13):5466-5473.
[25]	谭冲,刘颖杰,王薇,等.碳氮比对聚氨酯生物膜反应器短程硝化反硝化的影响[J]. 环境科学, 2014,35(10):3807-3813. Tan C, Liu Y J, Wang W, et al. Effect of carbon/nitrogen ratio on short-cut nitrification and denitrification of polyurethane biofilm reactor[J]. Environmental Science, 2014,35(10):3807-3813.
[26]	荣宏伟,彭永臻,张朝升,等.硝酸盐氮对反硝化除磷的影响及过程控制[J]. 北京工业大学学报, 2009,35(3):385-390. Rong H W, Peng Y Z, Zhang C S, et al. Effect of nitrate on denitrifying phosphorus removing and the process control[J]. Journal of Beijing University of Technology, 2009,35(3):385-390.
[27]	曹长青,雷中方,胡志荣,等.反硝化除磷过程中的影响因素探讨[J]. 中国给水排水, 2005,21(7):22-25. Cao C Q, Lei Z F, Hu Z R, et al. Discussion on the affecting factors of denitrifying phosphorus removal[J]. China Water & Wastewater, 2005, 21(7):22-25.
[28]	李锋民,单时,李媛媛,等.好氧/厌氧潜流湿地结构工艺优化[J]. 环境科学, 2012,33(2):436-441. Li F M, Shan S, Li Y Y, et al. Optimization of aerobic/anaerobic subsurface flow constructed wetlands[J]. Environmental Science, 2012,33(2):436-441.
[29]	王敬国.尼罗红在测定细菌细胞中聚-β-羟基丁酸和其他脂类贮藏物质中的应用[J]. 微生物学报, 1994,34(1):71-75. Wang J G. Application of Nile red in determination of poly-β- hydroxybutyric acid and other lipid storage substances in bacterial cells[J]. Acta Microbiologica Sinica, 1994,34(1):71-75.
[30]	Miao L, Wang S Y, Li B K, et al. Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate[J]. Water Research, 2016,100:405-412.
[31]	Zhao J, Wang X X, Li X Y, et al. Advanced nutrient removal from ammonia and domestic wastewaters by a novel process based on simultaneous partial nitrification-anammox and modified denitrifying phosphorus removal[J]. Chemical Engineering Journal, 2018,354: 589-598.
[32]	He Q, Zhang W, Zhang S, et al. Enhanced nitrogen removal in an aerobic granular sequencing batch reactor performing simultaneous nitrification, endogenous denitrification and phosphorus removal with low superficial gas velocity[J]. Chemical Engineering Journal, 2017, 326:1223-1231.