交流电场与<i>Fusarium</i> sp.A-2耦合对博落回修复铀污染土壤的强化作用

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Abstract Pot experiments were conducted to study the effects of the coupling between alternating current electric field (AC) and Fusarium sp. A-2 (A-2) fungi on the biomass, uranium (U) enrichment and enzyme activity of Macleaya cordata (P), as well as the organic acid content, bioavailable uranium and microbial community structure in the rhizosphere soil. The results showed that compared with AC+P+U, A-2+P+U, and P+U groups, the fresh weight, plant height and total dry weight of Macleaya cordata in AC+A-2+P+U group were significantly increased by 10.67%~45.76%, 26.87%~92.02% and 61.99%~159.98%, respectively, and the contents of the total superoxide dismutase (T-SOD) and catalase (CAT) were increased by 41.24%~133% and 15.35%~63.63%, respectively, and the oxalic acid was increased by 13.03%~157.09%. Compared with the A-2+P+U and P+U groups, the bioavailable uranium in soil in AC+A-2+P+U group was increased by 12.5% and 28.57%, respectively. In the AC+A-2+P+U groups, there were a large number of Fusarium fungi and Acidobacteria in rhizosphere soil, and the proportion of Fusarium fungi was the highest. Compared with AC+P+U, A-2+P+U, P+U, and A-2+U groups, the proportion of Fusarium fungi in AC+A-2+P+U group was increased by 16.67%, 81.03%, 299.23% and 374.47%, respectively. The mechanisms of AC and A-2 coupling enhanced the remediation of uranium contaminated soil by Macleaya cordata may be include that AC improved the mobility of uranium in soil, increased the contact between plant roots and uranium, and promoted the enrichment of uranium by Macleaya cordata; that AC stimulated the physiological activity and increased the biomass of Macleaya cordata, improved the activity of antioxidant enzymes, and enhanced the tolerance of Macleaya cordata to uranium; and that AC stimulated the growth of A-2 fungi and Acidobacteria, increased their proportion, and produced a large number of organic acids which can form chelates with uranium, reduced the stress of uranium on plants, increased the proportion of bioavailable speciation of uranium, and promoted the enrichment of uranium by Macleaya cordata. AC and A-2 coupling exhibited a significant enhancement effect on the remediation of uranium contaminated soil by Macleaya cordata, and there coupling was a promising enhancement method.

Key words： AC electric field Fusarium sp.A-2 fungi Macleaya cordata phytoremediation uranium contaminated soil

Received: 12 April 2021

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X53

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	DING De-Xin
	CHENG Hao
	LIU Jing-Jing
	DONG Xue
	LI Ai-Shu
	TAN Guo-Chi
	MA Jian-Hong
	ZHANG Hui
	WANG Yong-Dong
	HU Nan

Cite this article:

DING De-Xin,CHENG Hao,LIU Jing-Jing等. Enhancement effects of the coupling between AC electric field and Fusarium sp. A-2 on the remediation of uranium contaminated soil by Macleaya Cordata[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 258-266.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I1/258

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