内碳源短程反硝化启动及EPD-ANAMMOX耦合工艺性能

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Abstract This study was designed to evaluate performances of the start-up of endogenous partial denitrification and EPD-ANAMMOX process. The glycogen accumulation bacteria (GAO) were enriched in the anaerobic/aerobic sequence batch reactor (SBR) using the activated sludge as inoculum sludge and the acetate acted as carbon source by regulating the influent COD/P ratio as 150:1. Then, the GAOs were induced into denitrifying glycogen accumulation bacteria (DGAO) by means of gradually boosting the concentration of added nitrate at the end of anaerobic phase in SBR. Correspondingly, the level of chemical oxygen demand (COD) at the end of anoxic period was almost the same as that at the distal end of anaerobic stage, the total nitrogen removal efficiency and the average COD removal efficiency were over 98% and 86.74% at the end of anaerobic period of SBR, respectively. Furthermore, the endogenous partial denitrification system was successfully formed by shortening the anaerobic and anoxic time, the ratio of nitrite translated from nitrate (NTR) at the end of anoxic period was up to 65.96%. The average total nitrogen and COD removal load in the 30days operation of the coupled process were 0.222kgN/(m³×d) and 0.337kgCOD/(kgMLVSS.d) with an efficiency of 86.12% and 87.21%, respectively. Typically, the effluent NO₃^--N concentration was lower than 4.2mg/L and both NO₂^--N and NH₄⁺-N levels were near to 0mg/L in the same phase. Compared with inoculated sludge, the relative abundance of competibacter increased from 0.001% to 25.06% in the sludge of EPD-SBR during the stable operation period, and the total abundance of Defluviicoccus, Contendobacter, Sphingobium,and Amaricoccus increased from 0.14% to 0.431%, implying that the competibacter was the dominated functional bacteria for the endogenous partial denitrification system.

Key words： endogenous partial denitrification (EPD) the ratio of nitrite translated from nitrate denitrifying glycogen accumulation bacteria ANAMMOX

Received: 25 May 2021

PACS:

X703.5

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	ZHANG Xiao-ling
	ZHANG Meng
	CHEN Zi-wei
	ZHANG Zhuo

Cite this article:

ZHANG Xiao-ling,ZHANG Meng,CHEN Zi-wei等. Start-up of endogenous partial denitrification and performance of EPD-ANAMMOX coupling process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 601-611.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I2/601

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