间歇曝气-内循环生物滤池效能及生物膜特性

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Abstract The mechanism of enhanced denitrification by intermittent aeration-coupled internal circulation biofiltration is still not clear. We therefore investigated the pollutant removal in a biofiltration reactor. Several methods were used to analyze the biomass, biological activity, and nitrification and denitrification rates of the system, and the microbial population in the reactor was analyzed. The intensity of protein-like fluorescence gradually decreased throughout the system, and no protein-like peak was detected in the effluent. The decrease of NH₄⁺-N along the 10-50cm section of the reactor did not cause a significant increase in NO₃^--N, and the NO₃^--N content decreased at 50cm. This section showed obvious denitrification. The dissolved oxygen and biomass levels throughout the system indicated an anoxic/anaerobic environment and significant denitrification. In addition, the low oxygen uptake rate (OUR) but high TTC-dehydrogenase activity at 50cm and the significantly higher denitrification rate compared to the nitrification rate indicated enhanced denitrification in this region. Based on 16S rRNA high-throughput sequencing analysis, the system had a higher abundance of microorganisms involved in denitrification at the phylum level, mainly Firmicutes (10.64%) and Bacteroidetes (22.29%). Denitrification was also evident at the genus level in Comamonas (3.11%) and Hydrogenophaga (2.43%). Our results suggest that the BAF system with intermittent aeration coupled with internal circulation enhanced denitrification in the bottom zone and thus improved the denitrification efficiency.

Key words： biofilter intermittent aeration internal circulation pollutants microbial response

Received: 03 June 2021

PACS:

X703.5

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	Articles by authors
	REN Wu-ang
	CAO Feng-feng
	JU Kai
	JIN Peng-kang
	LI Si-min
	CHAI Bei-bei
	LEI Xiao-hui

Cite this article:

REN Wu-ang,CAO Feng-feng,JU Kai等. Intermittent aeration-internal circulation biological filter performance and biofilm characteristics[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 629-636.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I2/629

[1]	Dong J X, Wang Y H, Wang L J, et al. The performance of porous ceramsites in a biological aerated filter for organic wastewater treatment and simulation analysis[J]. Journal of Water Process Engineering, 2020,34:101134.
[2]	Zhang L L, Yue Q Y, Yang K L, et al. Enhanced phosphorus and ciprofloxacin removal in a modified BAF system by configuring Fe-C micro electrolysis:Investigation on pollutants removal and degradation mechanisms[J]. Journal of Hazardous Materials, 2018, 342(15):705-714.
[3]	杨永愿,汪晓军,赵爽,等.沸石曝气生物滤池短程硝化特性及其机制[J]. 中国环境科学, 2017,37(12):4518-4525. Yang Y Y, Wang X J, Zhao S, et al. Partial nitrification performance and mechanism of zeolite biological aerated filter for ammonium wastewater treatment[J]. China Environmental Science, 2017,37(12):4518-4525.
[4]	Feng Y, Li X, Song T, et al. Stimulation effect of electric current density (ECD) on microbial community of a three dimensional particle electrode coupled with biological aerated filter reactor (TDE-BAF)[J]. Bioresource Technology, 2017,243:667-675.
[5]	Wang H J, Dong W Y, Li T, et al. A modified BAF system configuring synergistic denitrification and chemical phosphorus precipitation:Examination on pollutants removal and clogging development[J]. Bioresource Technology, 2015,189:44-52.
[6]	江肖良,李孟,张少辉,等.4种不同工况生物滤池净化效能与微生物特性分析[J]. 环境科学, 2018,39(12):5503-5513. Jiang X L, Li M, Zhang S H, et al. Purification efficiency and microbial characteristics of four biofilters operated under different conditions[J]. Environmental Science, 2018,39(12):5503-5513.
[7]	Wang X J, Han J J, Chen Z W, et al. Combined processes of two-stage Fenton-biological anaerobic filter-biological aerated filter for advanced treatment of landfill leachate[J]. Waste Management, 2012,32(12):2401-2405.
[8]	Zhang Q, Wang C R, Jiang L X, et al. Impact of dissolved oxygen on the microbial community structure of an intermittent biological aerated filter (IBAF) and the removal efficiency of gasification wastewater[J]. Bioresource Technology, 2018,255:198-204.
[9]	田兆龙,汪晓军,黄志聪.间歇式曝气生物滤池对焚烧垃圾渗滤液深度脱氮的研究[J]. 环境科学学报, 2013,33(5):1244-1248. Tian Z L, Wang X J, Huang Z C. Nitrogen removal for waste incineration leachate by intermittent aerated biological filter[J]. Acta Scientiae Circumstantiae, 2020,33(5):1244-1248.
[10]	柴蓓蓓,曹锋锋,任武昂.间歇曝气耦合内循环曝气生物滤池脱氮除磷性能研究[J]. 环境污染与防治, 2020,42(12):1443-1448. Chai B B, Cao F F, Ren W A. Research on nitrogen and phosphorus removal performance of intermittent aeration coupled with internal circulation biological aerated filter[J]. Environmental Pollution & Control, 2020,42(12):1443-1448.
[11]	靖青秀,游威,黄晓东,等.钴渣基陶粒脱除曝气生物滤池废水中氮磷研究[J]. 水处理技术, 2020,46(10):28-32. Jing Q X, You W, Huang X D, et al. Study on removal of nitrogen and phosphorus in wastewater by a BAF with cobalt slag based ceramsite[J]. Technology of Water Treatment, 2020,46(10):28-32.
[12]	Bao T, Chen T H, Ezzatahmadi N, et al. A performance evaluation of a new iron oxide-based porous ceramsite (IPC) in biological aerated filters[J]. Environmental Technology, 2017,38(7):827-834.
[13]	Zhou H, Xu G. Biofilm characteristics, microbial community structure and function of an up-flow anaerobic filter-biological aerated filter (UAF-BAF) driven by COD/N ratio[J]. The Science of the Total Environment, 2020,708(15):1-10.
[14]	叶星,马凯迪,黄俊生,等.反硝化生物滤池中生物膜量与脱氮效果和脱氢酶活性的关系[J]. 环境工程学报, 2020,14(5):1210-1215. Ye X, Ma K D, Huang J S, et al. Relationship between biofilm mass and nitrogen removal efficiency dehydrogenase activity in the denitrification biological filter[J]. Chinese Journal of Environmental Engineering, 2020,14(5):1210-1215.
[15]	向红,刘武平,李璇,等.生物滤池中生物量与生物活性分析及其净水效果[J]. 中国给水排水, 2011,27(3):48-51. Xiang H, Liu W P, Li X, et al. Analysis of biological filter biomass and biological activity and its purification effect[J]. China Water & Wastewater, 2011,27(3):48-51.
[16]	国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2004. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2004.
[17]	王悦,王海,石烁辉,等.反硝化滤池反冲洗效能综合影响因素及微生物种群[J]. 中国给水排水, 2021,37(1):76-80. Wang R, Wang H, Shi S H, et al. Comprehensive influencing factors of backwashing efficiency of denitrifying biofilter and its microbial community[J]. China Water & Wastewater, 2011,37(1):76-80.
[18]	李川,郭鸣,王让,等.改性生物砂滤池对污染物的去除效果及微生物特性[J]. 中国给水排水, 2019,35(5):94-98. Li C, Guo M, Wang R, et al. Analysis of pollutants removal and microbial characteristics of modified biological sand filter[J]. China Water & Wastewater, 2019,35(5):94-98.
[19]	金幼平,杨雪英,陈罡,等.活性污泥INT-脱氢酶活性检测方法的改进[J]. 中国给水排水, 2016,32(22):153-156. Jin Y P, Yang X Y, Chen G, et al. Improvement of INT-dehydrogenase activity detection method of activated sludge[J]. China Water & Wastewater, 2016,32(22):153-156.
[20]	周圆,支丽玲,郑凯凯,等.城镇污水处理厂活性污泥反硝化速率的影响因素及优化运行研究[J]. 环境工程, 2020,38(7):100-108. Zhou Y, Zhi L L, Zheng K K, et al. Influencing factors and optimization analysis of denitrification rate in urban wastewater treatment plants[J]. Environmental Engineering, 2020,38(7):100-108.
[21]	Zhang X J, Zhou Y, Yu B Y, et al. Effect of copper oxide nanoparticles on the ammonia removal and microbial community of partial nitrification process[J]. Chemical Engineering Journal, 2017,328(15):152-158.
[22]	李燕飞,孙迎雪,田媛,等.曝气生物滤池处理生活污水研究[J]. 环境工程学报, 2011,5(3):575-578. Li Y F, Sun Y X, Tian Y, et al. Study on domestic wastewater treatment with biological aerated filter[J]. Chinese Journal of Environmental Engineering, 2011,5(3):575-578.
[23]	安莹,李云辉,李震,等.改良型AO法组合工艺中有机物的三维荧光分析[J]. 环境工程学报, 2013,7(1):159-163. An Y, Li Y H, Li Z, et al. Analysis of EEM fluorescence spectra of dissolved organic matter in improved AO combined process[J]. Chinese Journal of Environmental Engineering, 2013,7(1):159-163.
[24]	张华,全桂军,黄健,等.废水DOM荧光强度与COD总氮的相关分析[J]. 环境科学与技术, 2017,40(10):157-162. Zhang H, Quan G J, Huang J, et al. Analysis of the correlation between the fluorescence intensity of the dissolved organic matter and COD and total nitrogen in wastewater[J]. Environmental Science & Technology, 2017,40(10):157-162.
[25]	刘俊峰,范举红,刘锐,等.2种填料BAF深度处理印染废水沿程污染物变化规律研究[J]. 环境科学, 2014,35(12):4596-4601. Liu J F, Fan J H, Liu R, et al. Variation of pollutants along the height of two media BAF during advanced treatment of dyeing wastewater[J]. Environmental Science, 2014,35(12):4596-4601.
[26]	王曦曦,伦琳,张继彪,等.改进型曝气生物滤池的生物量和生物活性[J]. 环境工程学报, 2012,6(6):1829-1833. Wang X X, Lun L, Zhang J B, et al. Biomass and microbial activity in modified biological aerated filter[J]. Chinese Journal of Environmental Engineering, 2012,6(6):1829-1833.
[27]	Urfer D, Huck P M. Measurement of biomass activity in drinking water biofilters using a respirometric method[J]. Water Research, 2001,35(6):1469-1477.
[28]	王琳,肖娇玲,窦娜莎.Biostyr曝气生物滤池的沿程微生物多样性[J]. 环境工程学报, 2016,10(11):6283-6289. Wang L, Xiao J L, Dou N S. Microorganism characteristics along Biostyr biological aerated filter[J]. Chinese Journal of Environmental Engineering, 2016,10(11):6283-6289.
[29]	Gao P, Xu W, Sontag P, et al. Correlating microbial community compositions with environmental factors in activated sludge from four full-scale municipal wastewater treatment plants in Shanghai, China[J]. Applied Microbiology & Biotechnology, 2016,100(10):4663-4673.
[30]	Wang J F, Song X S, Wang Y H, et al. Nitrate removal and bioenergy production in constructed wetland coupled with microbial fuel cell:Establishment of electrochemically active bacteria community on anode-ScienceDirect[J]. Bioresource Technology, 2016,221:358-365.
[31]	Pereira A D, Leal C D, Dias M F, et al. Effect of phenol on the nitrogen removal performance and microbial community structure and composition of an anammox reactor[J]. Bioresource Technology, 2014,166:103-111.
[32]	Ma J, Wang Z, Yang Y, et al. Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454high-throughput pyrosequencing[J]. Water Research, 2013,47(2):859-869.
[33]	Rodriguez-Sanchez A, Margareto A, Robledo-Mahon T, et al. Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations[J]. The Chemical Engineering Journal, 2017,325:257-269.
[34]	刘亚妮,朱宏伟,黄荣新,等.曝气生态滤池中微生物群落组成及物种多样性[J]. 中国环境科学, 2020,40(3):1075-1080. Liu Y N, Zhu H W, Huang R X, et al. Microbial community composition and species diversity in ecological aerated filter[J]. China Environmental Science, 2020,40(3):1075-1080.
[35]	Na S, Ge C, Ahmad H A, et al. Realization of microbial community stratification for single-stage nitrogen removal in a sequencing batch biofilter granular reactor[J]. Bioresource Technology, 2017,241:681-691.
[36]	Chakravarthy S S, Pande S, Kapoor A, et al. Comparison of denitrification between Paracoccus sp. and Diaphorobacter sp[J]. Applied Biochemistry & Biotechnology, 2011,165(1):260-269.