鲴鲢鳙混养系统中微生物对氮素迁移转化的影响

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Abstract The polyculture system of Plagiogathops microlepis Bleeker (Xenocypris microlepis), Hypophthalmichthys molitrix (Silver carp) and Aristichthys nobilis (bighead carp) was set up in simulated eutrophic water. The dynamics of microbial communities and their effects on the migration and transformation of nitrogen in the sediments were investigated by applying isotopic labeling and microbial analysis to reveal the underlying mechanism of the polyculture system. The results showed that:The polyculture of Xenocypris microlepis, silver carp and bighead carp could increase the proportion of Pseudomonas and Bacillus involved in the ammonification process in the sediments. Comparing with the silver carp and bighead carp group, the total proportion of Bacillus and Pseudomonas in the polyculture group was the highest (31.2%) at the end of experiment, showing that it enhanced ammonification process and thus, reduced the content of organic nitrogen in the sediments. Consequently, the δ¹⁵N_{(substrate sediment)} of the polyculture group decreased to 726.8‰. The polyculture of Xenocypris microlepis, silver carp and bighead carp could increase the proportion of denitrifying bacteria (Zoogloea and Bacillus) in the sediments. The proportion of Zoogloea and Bacillus in the polyculture group was significantly higher than that in the silver carp and bighead carp group (P<0.05), reaching 8.17% and 33.7%, respectively. Therefore, the denitrification process was enhanced in the system, converting more nitrate into nitrogen gas in the water and finally releasing this gas from this water. At the end of the experiment, in the polyculture group, the δ¹⁵N_{(nitrate nitrogen)}decreased to 96.3‰ and the total content of ¹⁵N decreased to 6.94μmol.

Key words： Plagiogathops microlepis Bleeker, Hypophythalmichthys molitrix and Aristichthys nobilis polyculture stable isotope technique nitrogen microbial community

Received: 12 May 2021

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X524

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	ZHANG Zhe
	GAO Yue-xiang
	ZHANG Yi-min
	CHENG Ling-ling
	GUO Yan-min
	ZHU Yue-ming
	YU Jiang-hua

Cite this article:

ZHANG Zhe,GAO Yue-xiang,ZHANG Yi-min等. The effects of microorganisms on the migration and transformation of nitrogen in the polyculture system of Plagiogathops microlepis Bleeker, Hypophthalmichthys molitrix and Aristichthys nobilis[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 897-906.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I2/897

[1]	Xie P. Experimental studies on the role of planktivorous fishes in the elimination of Microcystis bloom from Donghu Lake using enclosure method[J]. Chinese Journal of Oceanology and Limnology, 1996, 14(3).
[2]	Starling FLRM. Control of eutrophication by silvercarp (Hypophthalmichthysmolitrix) in the tropical Paranoa Reservoir (Brazil):A mesocosm experiment[J]. Hydrobiologia, 1993,257:143-152.
[3]	Fuhrman J A. Microbial community structure and its functional implications[J]. Nature, 2009,459(7244):193-199.
[4]	韩菲尔.太湖水体浮游植物氮素吸收过程及其影响因素[D]. 苏州:苏州科技大学, 2019. Han F E. Phytoplankton nitrogen absorption process and its influencing factors in Taihu Lake[D]. University of Science and Technology Suzhou, 2019.
[5]	陈玲玲,高月香,张毅敏,等.鲢鱅鲴混养对水环境及氮素迁移转化的影响[J]. 中国环境科学, 2019,39(3):1181-1188. Chen L L, Gao Y X, Zhang Y M, et al. The effect of silver carp polyculture on water environment and nitrogen migration and transformation[J]. China Environmental Science, 2019,39(3):1181-1188.
[6]	陈军,王煜恒,王会聪,等.投放不同密度的细鳞斜颌鲴对河蟹养殖水质和经济效益的影响[J]. 江苏农业科学, 2017,45(23):174-177. Chen J, Wang Y H, Wang H C, et al. Effects of different densities on the water quality and economic benefits of Eriocheir sinensis[J]. Jiangsu Agricultural Sciences, 2017,45(23):174-177.
[7]	刘家辉.鲴鱼养殖效益分析[J]. 农村新技术, 2014(6):46-47. LIU J H. Benefit analysis of carp breeding[J]. New Rural Technology, 2014(6):46-47.
[8]	贡毅.池塘泥鳅、细鳞斜颌鲴、鳙鱼高效生态混养试验[J]. 渔业致富指南, 2016,(7):53-55. Gong Y. High-efficiency ecological polyculture experiment of pond loach, scaly carp, and bighead carp[J]. Fisheries Getting Rich Guide, 2016,(7):53-55.
[9]	Papaspyrou S, Gregersen T, Cox R P, et al. Sediment properties and bacterial community in burrows of the ghost shrimp Pestarella tyrrhena (Decapoda:Thalassinidea)[J]. Aquatic Microbial Ecology, 2005,38:181-190.
[10]	Kang C K, Choy E J, et al. Food web structure of a restored macroalgal bed in the eastern Korean peninsula determined by C and N stable isotope analyses[J]. Marine Biology:International Journal on Life in Oceans and Coastal Waters, 2008,153(6):1181-1198.
[11]	王银平,谷孝鸿,曾庆飞,等.食微囊藻干粉鱼类对水环境的影响及氮素迁移转化规律[J]. 湖泊科学, 2015,27(3):475-485. Wang Y P, Gu X H, Zeng Q F, et al. Effects of microcystis-eating dry meal fish on the water environment and the law of nitrogen migration and transformation[J]. Journal of Lake Sciences, 2015,27(3):475-485.
[12]	Lehmann M F, Bernasconi S M, Mckenzie J A. A method for the extraction of ammonium from freshwaters for nitrogen isotope analysis[J]. Analytical Chemistry, 2001,73(19):4717-4721.
[13]	吴俊森,王丽丽,贾瑞宝,等.同位素分析法测定硝酸盐氮预处理方法研究[J]. 山东建筑大学学报, 2012,27(1):8-10. Wu J S, Wang L L, Jia R B, et al. Study on pretreatment for measurement nitrate nitrogen in isotopic analysis method[J]. Journal of Shandong Jianzhu University, 2012,27(1):8-10.
[14]	李柯.蓝藻分解所释放营养盐在沉水植被区的归趋[D]. 武汉:华中农业大学, 2011. Li K. The fate of nutrients released by the decomposition of cyanobacteria in submerged vegetation areas[D]. Wuhan:Huazhong Agricultural University, 2011.
[15]	赖江山.生态学多元数据排序分析软件Canoco5介绍[J]. 生物多样性, 2013,21(6):765-768. LAI J S. Introduction of Canoco5, a software for sorting and analyzing ecological multivariate data[J]. Biodiversity Science, 2013,21(6):765-768.
[16]	李晓,李冰,董玉峰,等.精养团头鲂池塘沉积物微生物群落的结构特征及组成多样性分析[J]. 水产学报, 2014,38(2):218-227. Li X, Li B, Dong Y F, et al. Analysis of sediment microbial communities in Megalobrama amblycephala intensive rearing pond[J]. Journal of Fisheries of China, 2014,38(2):218-227.
[17]	Dörte A, Stief P, Amann R, et al. In situ distribution and activity of nitrifying bacteria in freshwater sediment[J]. Environmental Microbiology, 2010,5(9):798-803.
[18]	Yang C, Zhang W, Liu R, et al. Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants[J]. Environmental & Technology, 2011,45(17):7408-15.
[19]	杨柳燕,王楚楚,等.淡水湖泊微生物硝化反硝化过程与影响因素研究[J]. 水资源保护, 2016,32(1):12-22,50. Yang L Y, Wang C C, et al. Study on the process and influencing factors of microbial nitrification and denitrification in freshwater lakes[J]. Water Resources Protection, 2016,32(1):12-22,50.
[20]	Flynn K J, Hipkin C R, Fasham M J R. Modelling the interactions between ammonium and nitrate uptake in marine phytoplankton[J]. Philosophical Transactions of the Royal Society of London, Series B. Biological Sciences, 1997,352(1361):1625-1645.
[21]	杨四秀.鲴鱼的生物学特性及养殖技术[J]. 内陆水产, 2004,29(5):28-28. Yang S X. Biological characteristics and breeding technology of scorpionfish[J]. Current Fisheries, 2004,29(5):28-28.
[22]	陈少莲,刘肖芳.论鲢,鳙对微囊藻的消化利用[J]. 水生生物学报, 1990,14(1):49-59. Chen S L, Liu XF. On the digestion and utilization of Microcystis by silver carp and bighead carp[J]. Acta Hydrobiologica Sinica, 1990, 14(1):49-59.
[23]	罗专溪,魏群山,王振红,等.淡水水体溶解有机氮对有毒藻种的生物有效性[J]. 生态环境学报, 2010,19(1):45-45. Luo Z X, Wei Q S, Wang Z H, et al. Bioavailability of dissolved organic nitrogen in freshwater to poisonous algae species[J]. Ecology and Environmental Sciences, 2010,19(1):45-45.
[24]	Jones D L, Shannon D, Murphy D V, et al. Role of dissolved organic nitrogen (DON) in soil N cycling in grassland soils[J]. Soil Biology & Biochemistry, 2004,36(5):749-756.
[25]	Jones D L, Kielland K. Amino acid, peptide and protein mineralization dynamics in a taiga forest soil[J]. Soil Biology & Biochemistry, 2012,55:60-69.
[26]	Bach H J, Munch J C. Identification of bacterial sources of soil peptidases[J]. Biology and Fertility of Soils, 2000,31(3/4):219-224.
[27]	Watanabe K, Hayano K. Source of soil protease based on the splitting sites of a polypeptide[J]. Soil Science and Plant Nutrition, 1994,40(4):697-701.
[28]	赵彤,蒋跃利,闫浩,等.土壤氨化过程中微生物作用研究进展[J]. 应用与环境生物学报, 2014,20(2):315-21. Zhao T, Jiang Y L, Yan H, et al. Research progress of microbial action in the process of soil ammonification[J]. Chinese Journal of Applied and Environmental Biology, 2014,20(2):315-21.
[29]	Li Y, Miao Y, Zhang W, et al. Sertraline inhibits top-down forces (predation) in microbial food web and promotes nitrification in sediment[J]. Environmental Pollution, 2020,267:115580.
[30]	Kessel M V, Speth D R, Albertsen M, et al. Complete nitrification by a single microorganism[J]. Nature, 2015,528(7583).
[31]	Ma, Yuexin Y, Zichao D, et al. Characterization of Bacterial Community, Ammonia-Oxidizing Bacteria, and Nitrospira During the Operation of a Commercial-Scale Recirculating Aquaculture System for Culturing Pufferfish Takifugu rubripes[J]. Journal of Ocean University of China, 2020,19(6):183-192.
[32]	Sybil P. Seitzinger. Denitrification in freshwater and coastal marine ecosystems:Ecological and geochemical significance[J]. Limnology and Oceanography, 1988,33(4part2):702-724.
[33]	Yao L, Jiang X L, Chen C R, et al. Within-lake variability and environmental controls of sediment denitrification and associated N2O production in a shallow eutrophic lake[J]. Ecological engineering:The Journal of Ecotechnology, 2016,97251-97257.
[34]	高春娣,张娜,韩徽,等.低温下丝状菌膨胀污泥的微生物多样性[J]. 环境科学, 2020,41(7):3373-3383. Gao C D, Zhang N, Han H, et al. Microbial diversity of filamentous bacteria expanded sludge at low temperature[J]. Environmental Science, 2020,41(7):3373-3383.
[35]	李公伟.准好氧生物反应器填埋场微生物群落多样性及N2O产生途径研究[D]. 青岛:青岛理工大学, 2018. Li G W. Research on microbial community diversity and N2O generation pathway of semi-aerobic bioreactor landfill[D]. Qingdao:Qingdao Technological University, 2018.
[36]	Yang T, Xin Y, Zhang L, et al. Characterization on the aerobic denitrification process of Bacillus strains[J]. Biomass and Bioenergy, 2020,140:105677.
[37]	蒙海林,刘复荣,何敬愉,等.异养硝化细菌的分离鉴定及组合菌群硝化性能分析[J]. 华南理工大学学报(自然科学版), 2019,47(9):113-120. Meng H L, Liu F R, He J Y. Isolation and identification of heterotrophic nitrifying bacteria and analysis of nitrification performance of combined bacteria[J]. Journal of South China University of Technology (Natural Science Edition), 2019,47(9):113-120.
[38]	Stief P, Polerecky L, Poulsen M, et al. Control of nitrous oxide emission from Chironomus plumosus larvae by nitrate and temperature[J]. Limnology & Oceanography, 2010,55(2):872-884.
[39]	Gilbertson W W, Martin S, Prosser J I. Differential effects of microorganism-invertebrate interactions on benthic nitrogen cycling[J]. Fems Microbiology Ecology, 2012,(1):11-22.
[40]	Stief, P. Stimulation of microbial nitrogen cycling in aquatic ecosystems by benthic macrofauna:mechanisms and environmental implications[J]. Biogeosciences, 2013,10(12):7829-7846.
[41]	Marinelli R L, Lovell C R, Wakeham S G. Experimental investigation of the control of bacterial community composition in macrofaunal burrows[J]. Marine Ecology Progress, 2002,235:1-13.