紫外灭活水中3种致病性曲霉的效能及其光复活控制

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Abstract Three kinds of Aspergillus spores(Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus) commonly found in drinking water were used as research objects, and UV was used as the disinfection method to study their inactivation efficiency and mechanism. Control of photoreactivation for three Aspergillus spores inactivated by UV was also evaluated. The different inactivation degree was due to the different size, pigment and hydrophobicity. The resistance of the three Aspergillus spores inactivated by UV was:Aspergillus flavus > Aspergillus niger> Aspergillus fumigatus. In addition, the inactivation rate constants k of the three Aspergillus spores were consistent with the Chick-Watson model, in which the k of Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus were 0.027, 0.026 and 0.031 cm2/m J, respectively. The order of the inactivation rate constant k of the three Aspergillus spores was the same as the difficulty of inactivation. UV penetrates cell wall and cell membrane, which blocks DNA replication and transcription, ultimately causing membrane damage and the increase of intracellular high ROS level. After inactivation, the spores showed obvious sinking and wrinkle on the surface. Photoreactivation rate constant and maximum survival ratio of the three Aspergillus spores: Aspergillus niger>Aspergillus flavus > Aspergillus fumigatus. The discrepancy of photoreactivation among the three Aspergillus species was caused by the difference of the number and activity of intracellular photolyase.

Key words： UV fungal spores Aspergillus disinfection mechanism photoreactivation

Received: 10 August 2021

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X523

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	Articles by authors
	WU Ge-hui
	ZHAO Hui
	WAN Qi-qi
	XU Xiang-qian
	CAO Rui-hua
	HUANG Ting-lin
	WEN Gang

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WU Ge-hui,ZHAO Hui,WAN Qi-qi等. Inhibit the photoreactivation of three pathogenic Aspergillus spores in water by UV: kinetics and mechanism[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1173-1181.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I3/1173

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