泗顶矿区剖层土固氮微生物群落结构和丰度

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Abstract In the present study, twelve soil profile samples (4 soil layers in each area) were collected from upstream, downstream and mine tailing areas in the Siding mine located in Liuzhou, Guangxi Province. The community composition, abundance and diversity of diazotrophs from the soil profile were examined using Illumina MiSeq high-throughput sequencing technology and fluorogenic quantitative real-time PCR technology. The results indicated that the phylum Proteobacteria was the most dominant taxon, with an abundance higher than 70%. Alphaproteobacteria was the dominant class in the soil profile from the upstream and downstream areas. The nifH gene abundance in the soil profile ranged from 3.02×10⁶~1.17×10⁷, 2.55×10⁶~7.78×10⁶and 8.19×10⁵~3.14×10⁶ gene copies/g (DW) in upstream, downstream and mine tailing areas, respectively. Nitrogen-related soil properties (including total nitrogen, ammonia and nitrate) and phosphorus-related soil properties (including total phosphorus and available phosphorus) were the main factors influencing nifH gene abundance. Soil lead, zinc and cadmium concentrations were found to mainly influence diazotrophic community composition. The Shannon index and ACE index of the diazotrophic community in upstream area were higher than those in the downstream and mine tailing areas, which indicated that the diversity and richness of the diazotrophic community in the soil profile were relatively higher in the upstream area. In addition, the soil potassium, calcium and sodium contents contributed to the ACE index and NMDS1index of the diazotrophic community to different degrees. Hence, the results indicated that variation in soil environmental factors had an impact on the community composition, abundance and diversity of diazotrophs from the soil profile. Our research will help to provide a scientific basis for nitrogen regulation, ecological restoration and reconstruction in mining areas.

Key words： mine area diazotrophs soil profile community structure nifH gene abundance

Received: 16 September 2021

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X172

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	LI Yi
	ZHANG Hai-chun
	LIU Yuan
	WEI Jiao-teng
	WANG Cong
	LIANG Ying
	LIU Ke-hui
	YU Fang-ming

Cite this article:

LI Yi,ZHANG Hai-chun,LIU Yuan等. Characteristics on the community structure and abundance of diazotrophs from the soil profile in the Siding mine area[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1819-1828.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I4/1819

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