强污泥流失对anammox工艺脱氮性能及菌群的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (0 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to study the effects of strong sludge loss on the operation performance, characteristics of granular sludge and nitrogen removal bacteria and functional genes in anaerobic ammonia oxidation (anammox) reactor, a upflow anaerobic sludge blanket (UASB) system was constructed and operated to treat synthetic wastewater. Experimental results showed that the nitrogen removal performance of anammox process was not obviously impaired by high sludge loss, and the total nitrogen (TN) removal efficiency (RE) reached 89.18% after 4days’ recovery. A higher EPS content and a lower PN/PS value of 0.12were beneficial to the formation and agglomeration of anammox granular sludge. The anammox granular sludge with sludge size of > 2mm accounted for 44.9% of the total sludge in this system, and corresponding value was 84.3% for the sludge with size of > 0.5mm, which was advantage to the sludge retention in the reactor. The dominant anammox phyla were Proteobacteria (28.03%), Planctomycetes (15.57%) and Chloroflexi (8.63%), and the domimant genera were anammox bacteria Candidatus Jettenia (9.63%) and Candidatus Brocadia (3.54%). The functional genes involved in anammox reaction included nirS (1.27%), hzs (1.28%), hzo (1.29%), hao (7.04%) and hdh (0.81%), but the presence of denitrification bacteria and related functional genes decreased the stoichiometric ratios Rs (ΔNO₂^--N/ΔNH₄⁺-N) and Rp (ΔNO₃^--N/ΔNH₄⁺-N) of anammox reaction.

Key words： anaerobic ammonia oxidation sludge loss sludge characteristics bacterial community functional genes

Received: 25 October 2021

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHAO Yi-chun
	LIU Ya-lei
	LI Yi-shu
	FANG Yuan-yuan
	WANG Bo-han
	LI Jia-rui
	WU Dong
	CHANG Ben-ze
	YANG Guang-feng

Cite this article:

ZHAO Yi-chun,LIU Ya-lei,LI Yi-shu等. Effects of high sludge loss on the nitrogen removal performance and microbial community of anammox process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2161-2168.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I5/2161

[1]	You Q G, Wang J H, Qi G X, et al. Anammox and partial denitrification coupling: a review [J]. RSC Advances, 2020,10: 12554-12572.
[2]	Liu Y X, Liu W, Li Y Y, et al. Layered inoculation of anaerobic digestion and anammox granular sludges for fast start-up of an anammox reactor [J]. Bioresource Technology, 2021,339(11):125573.
[3]	Li Y, Fan T, Xu D, et al. Advances in biological nitrogen removal of landfill leachate [J]. Sustainability, 2021,13(11):6236.
[4]	Ren Y, Ngo H H, Guo W S, et al. New perspectives on microbial communities and biological nitrogen removal processes in wastewater treatment systems [J]. Bioresource Technology, 2019,297(8):122491.
[5]	Wei Y, Jin Y, Zhang W J. Domestic Sewage Treatment Using a one-stage ANAMMOX process [J]. International Journal of Environmental Research and Public Health, 2020,17(9):3284.
[6]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms [J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[7]	陈重军,王瑶琪,姜滢,等.有机物对连续流Anammox脱氮及微生物群落影响 [J]. 中国环境科学, 2019,39(12):5049-5055. Cheng C J, Wang Y Q, Jiang Y, et al. Effects of organic matter on nitrogen removal and microbial community in anammox reactor [J]. China Environmental Science, 2019,39(12):5049-5055.
[8]	Wang Y, Ji X M, Jin R C. How anammox responds to the emerging contaminants: Status and mechanisms [J]. Journal of Environmental Management, 2021,293:112906.
[9]	Kocamemi B A, Dityapak D, Semerci N, et al. Anammox start-up strategies: the use of local mixed activated sludge seed versus anammox seed [J]. Water Science & Technology, 2018,78(9):1901– 1915.
[10]	Zheng B Y, Zhang L, Guo J H, et al. Suspended sludge and biofilm shaped different anammox communities in two pilot-scale one-stage anammox reactors [J]. Bioresource Technology, 2016,211:273-279.
[11]	Niederdorfer R, Hausherr D, Palomo A, et al. Temperature modulates stress response in mainstream anammox reactors.[J]. Communications Biology, 2021,4(1):23.
[12]	Adams M, Xie J X, Kabore A W J, et al. Research advances in anammox granular sludge:a review [J]. Critical Reviews in Environmental Science and Technology, 2020,(4):1-44.
[13]	Lin Q J, Kang D, Zhang M, et al. The performance of anammox reactor during start-up: enzymes tell the story [J]. Process Safety and Environmental Protection, 2019,121:247-253.
[14]	Zhu G B, Wang S Y, Ma B, et al. Anammox granular sludge in low-ammonium sewage treatment: Not bigger size driving better performance [J]. Water Research, 2018,142:147-158.
[15]	国家环境保护总局.水和废水监测分析方法 [M]. 4版.北京:中国环境科学出版社, 2002:227-285. The State Environmental Protection Administration. Water and wastewater monitoring and analysis method [M]. 4th Edition. Beijing: China Environmental Science Press, 2002:227-285.
[16]	顾澄伟.厌氧氨氧化颗粒污泥培养及其颗粒特性研究 [D]. 苏州:苏州科技大学, 2019. Gu C W. The Cultivation and features of anammox granular sludge [D]. Suzhou: Suzhou University of Science and Technology, 2019.
[17]	Adav S S, Lee D J. Extraction of extracellular polymeric substances from aerobic granule with compact interior structure [J]. Journal of Hazardous Materials, 2008,154(1-3):1120-1126.
[18]	Luo J H, Hao T W, Wei L, et al. Impact of influent COD /N ratio on disintegration of aerobic granular sludge [J]. Water Research, 2014, 62:127-135.
[19]	朱晓桐,于冰洁,林久淑,等.ANAMMOX-UASB反应器启动特性 [J]. 环境科学与技术, 2020,43(12):143-150. Zhu X T, Yu B J, Lin J S, et al. Startup characteristics of ANAMMOX-UASB reactor [J]. Environmental Science & Technology [J]. Environmental Science & Technology, 2020,43(12): 143-150.
[20]	Wang D P, He Y, Zhang X X. A comprehensive insight into the functional bacteria and genes and their roles in simultaneous denitrification and anammox system at varying substrate loadings [J]. Applied Microbiology and Biotechnology, 2019,103(3):1523-1533.
[21]	唐崇俭,郑平,陈建伟,等.中试厌氧氨氧化反应器的启动与调控 [J]. 生物工程学报, 2009,25(3):406-412. Tang C J, Zheng P, Chen J W, et al. Start-up and process control of a pilot-scale Anammox bioreactor at ambient temperature [J]. Chinese Journal of Biotechnology, 2009,25(3):406-412.
[22]	Lotti T, Carretti E, Berti D, et al. Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge [J]. Journal of Environmental Management, 2019,236(15):649-656.
[23]	Wang W G, Yan Y, Zhao Y H, et al. Characterization of stratified EPS and their role in the initial adhesion of anammox consortia [J]. Water Research, 2020,169:115223.
[24]	McSwain B S, Irvine R L, Hausner M, et al. Composition and distribution of extracellular polymeric substances in aerobic flocs and granular sludge [J]. Applied and Environmental Microbiology, 2005, 71(2):1051-1057.
[25]	Wang S, Huang X X, Liu L J, et al. Understanding the mechanism in aggregation ability between aerobic and anammox granular sludge from the perspective of exopolysaccharides [J]. Journal of Water Process Engineering, 2020,38:101629.
[26]	唐崇俭,郑平,汪彩华,等.高负荷厌氧氨氧化EGSB反应器的运行及其颗粒污泥的ECP特性 [J]. 化工学报, 2010,61(3):732-739. Tang C J, Zheng P, Wang C H, et al. Granulation and characteristics of extracellular polymers of anammox sludge in high-load EGSB reactor [J]. CIESC Journal, 2010,61(3):732-739.
[27]	陈方敏,顾澄伟,胡羽婷,等.厌氧氨氧化污泥恢复过程中的颗粒特性 [J]. 环境科学, 2018,39(12):319-325. Chen F M, Gu C W, Hu Y T, et al. Granular characteristics of anaerobic ammonia oxidation sludge during the recovery process [J]. Environmental Science, 2018,39(12):319-325.
[28]	Liu S, Lin C, Diao X X, et al. Interactions between tetracycline and extracellular polymeric substances in anammox granular sludge [J]. Bioresource Technology, 2019,293:122069.
[29]	Liu L J, Ji M, Wang F, et al. Microbial community shift and functional genes in response to nitrogen loading variations in an anammox biofilm reactor [J]. International Biodeterioration & Biodegradation, 2020,153:105023.
[30]	Yu M, Liao R H, Zhang X X, et al. Metagenomic insights into Cr (VI) effect on microbial communities and functional genes of an expanded granular sludge bed reactor treating high-nitrate wastewater [J]. Water Research, 2015,76:43-52.
[31]	Ismail S, Elreedy A, Elsamadony M, et al. Response of anammox bacteria to short-term exposure of 1,4-dioxane: Bacterial activity and community dynamics [J]. Separation and Purification Technology, 2021,266:118539.
[32]	Zhang Z, Liu S. Insight into the overconsumption of ammonium by ANAMMOX consortia under anaerobic conditions [J]. Journal of Applied Microbiology, 2014,117(6):1830-1838.
[33]	黄郑郑,曹刚,李紫惠,等.XH02菌强化反应器脱氮过程中菌群结构的高通量分析 [J]. 中国环境科学, 2017,37(5):1922-1929. Huang Z Z, Cao G, Li Z H, et al. High-throughput sequencing analysis of community structure in reactor enhanced by heterotrophic nitrification-aerobic denitrification bacteria XH02 [J]. China Environmental Science, 2017,37(5):1922-1929.
[34]	Alyne D P, Angela C, Claudia E, et al. Microbial communities in anammox reactors: a review [J]. Environmental Technology Reviews, 2017,6(1):74-93.
[35]	Li Q Y, Chen J H, Liu G H, et al. Effects of biotin on promoting anammox bacterial activity [J]. Scientific Reports, 2021,11(1):2038.
[36]	Zhao Y P, Jiang B, Tang X, et al. Metagenomic insights into functional traits variation and coupling effects on the anammox community during reactor start-up [J]. Science of the Total Environment, 2019, 687:50-60.
[37]	王海月,彭玲,毛念佳,等.三价铁对有机物存在下厌氧氨氧化脱氮的影响 [J]. 中国环境科学, 2021,41(4):1672-1680. Wang H Y, Peng L, MaoN J, et al. Effect of Fe3+ on nitrogen removal of Anammox in the presence of organic matter [J]. China Environmental Science, 2021,41(4):1672-1680.
[38]	Peeters S H, Niftrik L V. Trending topics and open questions in anaerobic ammonium oxidation [J]. Current Opinion in Chemical Biology, 2019,49:45-52.
[39]	赵弋戈,郑平.厌氧氨氧化体的组成、结构与功能 [J]. 微生物学报, 2016,56(1):8-18. Zhao Y G, Zheng P. Composition, structure and function of anammox [J]. Acta Microbiologica Sinica, 2016,56(1):8-18.
[40]	周英杰,王淑梅,张兆基,等.厌氧氨氧化菌的代谢途径及其关键酶的研究进展 [J]. 生态学杂志, 2012,31(3):738-744. Zhou Y J, Wang S M, Zhang Z J, et al. Metabolic pathways of anammox bacteria and related key enzymes: a review [J]. Chinese Journal of Ecology, 2012,31(3):738-744.
[41]	常尧枫,郭萌蕾,谢军祥,等.厌氧氨氧化脱氮除碳功能菌群结构及代谢途径 [J]. 中国环境科学, 2022,42(3):1138-1145. Chang Y F, Guo M L, Xie J X, et al. The structure and metabolic pathway of functional bacteria for nitrogen and carbon removal in Anammox [J]. China Environmental Science, 2022,42(3):1138-1145.
[42]	Wang Z B, Ni S Q, Zhang J, et al. Gene expression and biomarker discovery of anammox bacteria in different reactors [J]. Biochemical Engineering Journal, 2016,115:108-114.