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Study on regulating anaerobic fermentation for producing short-chain fatty acids from primary sludge in WWTPs by different bio-enzymes |
LIU Guo-hua, WANG Jian, QI Lu, WANG Hong-chen |
Low-Carbon Water Environmental Technology Center, School of Environment, Renmin University of China, Beijing 100872, China |
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Abstract The objective of this study was to estimate the efficiency of SCFA production from primary sludge using bio- enzyme regulation including alkaline protease, neutral protease and α-amylase. The regulation mechanism was revealed by microbial community and SCFAs component analyses. The three kinds of bio-enzymes could enhance the hydrolysis and acid production during the fermentation of primary sludge. Compared with the blank group, the production and yield of SCFAs achieved 1508 mg COD/L and 0.174 g COD/g VSS on the fourth day of fermentation, respectively, increasing by 1129 mg COD/L and 0.13 g COD/g VSS. Microbial community structure analysis showed that the relative abundance of fermentation-relating bacteria such as Lentimicrobium, Proteiniphilum and Bacteroides were improved, and the growth of methanogenic archaea such as Methanosaeta and Methanosapirillum was inhibited when bio-enzymes were added into the fermentation system. At the same time, the production of acetic acid in the regulated fermentation process was also found to promote.
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Received: 14 September 2021
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