反硝化除磷工艺实现亚硝酸盐积累的参数优化

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Abstract To promote the coupling of denitrification phosphorus removal and anaerobic ammonia oxidation process, and achieve simultaneous efficient removal of nitrogen and phosphorus from wastewater, a sequencing batch reactor (SBR) was constructed to optimized the operational parameters for the nitrite accumulation by denitrifying phosphorus removal process. Under anaerobic-anoxic-microaerobic operational conditions, SBR gradually realized nitrite accumulation during denitrifying phosphorus removal process by feeding synthetic nitrate industrial wastewater in the anoxic section. The results showed that after 142 days of cultivation and domestication, when the influent C/P ratio of 55 and the nitrate concentration in the anoxic section was 23mg/L, the nitrite accumulation rate achieved 51.01%, the NO₃^--N→NO₂^--N transformation rate was 40.22%, nitrate removal rate was 72.14% and the highest removal rate of PO₄^3--P was 88.17%. The effluent COD concentration was lower than 25mg/L, and COD removal rate was maintained above 90%. Analysis of the microbial community structure showed that Bacteroidetes, Proteobacteria, Chloroflexi and Acidobacteria were the dominant phylum in the system. Through parameter optimization, the abundance of denitrifying phosphorus accumulating organisms represented by Candidatus Accumulibacter increased (the cumulative abundance increased from 1.49% to 5.08%), and a more remarkable increase in the abundance of glycogen accumulating organisms represented by Candidatus Competibacter (from 1.02% to 15.49%). The combined effects of phosphorus accumulating organisms and glycogen accumulating organisms contributed to the accumulation of nitrite in the phosphorus removal process.

Key words： denitrifying phosphorus removal partial denitrification nitrite accumulation microbial community

Received: 10 November 2021

PACS:

X703.5

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	Articles by authors
	DI Fei
	SUI Qian-wen
	GAO Chao-long
	ZHONG Hui
	XU Dong-yao
	WEI Yuan-song

Cite this article:

DI Fei,SUI Qian-wen,GAO Chao-long等. Parameter optimization for the achievement of nitrite accumulation by denitrifying phosphorus removal process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2647-2655.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I6/2647

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