硫自养反硝化颗粒表面与间隙微生物群落特征和基因分布

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Abstract Bioinformation including microbial community structure, functional genes and metabolic pathways of surface and interstitial biofilms in the particle elemental sulfur autotrophic denitrification column were investigated in the present study. Bacterial diversity of microorganisms in the surface biofilm of sulfur particles was lower than that of interstitial biofilm. Differences in the abundance of functional genes for nitrogen metabolism were significant. Abundance of extracellular nitrate and nitrite transportation genes in the interstitial biofilm was much higher than that in the surface biofilm, with the abundance of 0.0792%, 0.109% and 0.0157%, 0.0314%. For reductive denitrification metabolism, the total gene abundance in surface biofilm was significantly lower than that in interstitial biofilm, with their abundance of 0.367%、0.406% respectively. Moreover, abundances of genes participated in denitrification process were remarkably different, especially the genes in the process of reducing nitrate to nitrite and nitrous oxide to nitrogen gas. Regarding sulfur metabolism, no obvious difference was observed. APS (adenosyl phosphosulfate) oxidation was the major pathway for oxidation of sulfite to sulfate, with its gene abundance was much higher than the direct oxidation pathway, and their abundances were 0.137% and 0.0005% (surface), 0.138% and 0.0007% (interstitial). Results indicated that microorganisms in interstitial biofilms cooperated with that in the surface biofilms, contributing to sulfur autotrophic denitrification synergistically.

Key words： sulfur autotrophic denitrification biofilm community structure functional gene

Received: 23 November 2021

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X172

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	Articles by authors
	Lü Xiao-mei
	WU Yi-cong
	CHEN Gui-lian
	XU Jian-hui
	LIU Peng
	HU jun-jie

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Lü Xiao-mei,WU Yi-cong,CHEN Gui-lian等. Community structure and gene distribution of the surface and interstitial biofilm in the particle sulfur autotrophic denitrification[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2764-2770.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I6/2764

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