两种生物除磷系统活性污泥中除磷菌的甄别——淀粉-缺氧/好氧交替与乙酸盐-厌氧/好氧交替系统

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Abstract In order to identify phosphorus accumulating organisms(PAOs) in situ from activated sludge. 4', 6-diamidino-2- phenylindole (DAPI) staining and flow cytometry fluorescence sorting (FACS) were used to sceen the PAOs from the two sludge at the anoxic/aerobic SBR system (R1) with starch as the only carbon source and the anaerobic/aerobic system (R2) with acetate as the only carbon source. The species of the sorted bacteria were identified by 16S rRNA high-throughput sequencing. The results showed that in the R1, biological phosphorus removal was carried out in both the anoxic periods and aerobic periods. The phosphorus uptake in the anoxic period was greater than that in the aerobic period. In the R2, the phosphorus release in the anaerobic period and a large amount of phosphorus absorption in the aerobic period occurs. Results of the in situ fluorescence staining showed that 10⁶ bacteria with a relative purity of 85% with phosphorus-accumlating cell were sorted from the R1 and the R2. The sequencing results showed that in the R1, the dominant genera of PAOs in the anoxic periods were Halomonas (37.75%), unclassified Brucellaceae (14.15%), Pseudomonas (6.49%), unclassified Chlamydiales (0.027%) and Sphingopyxis (0.007%). The dominant PAOs in the aerobic periods were Halomonas (19.72%), unclassified Brucellaceae (14.62%), Pseudomonas (14.28%), unclassified Comamonadaceae (0.046%), unclassified Acidobacteria Gp3 (0.036%) and Ferruginibacter (0.026%). In the R1, unclassified Chlamydiales and Sphingopyxis only had phosphorus accumulation function under anoxic periods, while unclassified Comamonadaceae, unclassified Acidobacteria Gp3 and Ferruginibacter only had phosphorus accumulation function under aerobic periods. In the R2, the dominant PAOs were Dechloromonas (11.06%), unclassified Anaerolineaceae (9.29%), unclassified Bacteroidetes (7.44%), unclassified Gammaproteobacteria (7.34%) and Acinetobacter (0.31%). This means that in the R1, the bacteria involved in the phosphorus removal process include aerobic, anoxic and both aerobic and anoxic phosphorate accumulating bacteria three types. In the R2, the bacteria involved in the phosphorus removal process are only aerobic phosphorus accumulating bacteria.

Key words： phosphorus accumulating bacteria SBR flow cytometry fluorescence sorting technology high-throughput sequencing

Received: 10 February 2022

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X703.5

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	ZHOU Xu-hong
	YUAN Lin-jiang
	CHEN Xi
	YANG Rui
	ZHU Miao
	NAN Ya-ping
	HE Xiang-feng
	CHEN Yong

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ZHOU Xu-hong,YUAN Lin-jiang,CHEN Xi等. Screening of phosphorus removing bacteria from activated sludge for biological phosphorus removal: starch-anoxic/aerobic alternation and acetate-anaerobic/aerobic alternation system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4166-4173.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I9/4166

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