<i>Martelella</i> sp. AD-3强化活性污泥耐盐降解菲效能

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Abstract A halotolerant bioreactor with high efficiency degrading phenanthrene was built based on inoculating halophilic Martelella sp. AD-3 with municipal activated sludge. At the salinity of 3.0%, phenanthrene removal ratio reached 97% with influent concentration of 20mg/L. Batch experiments showed that salinity of 3.0%, pH of 7.5~8.5 and initial concentrations of 20~200mg/L are the optimal conditions for phenanthrene degradation, under which the specific activity of the sludge exceeded 1.0mg/(gVSS×h). Yeast extract and phenol can promote the degradation of phenanthrene while cadmium and cyanide would inhibit the process. Sodium acetate, copper and chromium had no obvious effect on phenanthrene degradation. 16S rRNA gene high-throughput sequencing showed that the relative abundance of strain AD-3 had long-term stability in the reactor. Shinella, Microbacterium and Brevundimonas were the dominant bacteria with their relative abundances of 20.7%, 15.1% and 11.9% respectively. RT-qPCR results showed that after inoculation with strain AD-3, the fold change of PAHs dioxygenase functional gene encoding RHDa increased from 2.1 to 11.7.

Key words： halotolerant activated sludge phenanthrene degradation environmental conditions microbial community functional gene high-throughput sequencing

Received: 18 February 2022

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X703

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	WANG Yu
	LI Wei
	CUI Chang-zheng
	LIU Yong-di

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WANG Yu,LI Wei,CUI Chang-zheng等. Martelella sp. AD - 3 enhanced the degradation of phenanthrene in a halotolerant activated sludge system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4226-4231.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I9/4226

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