光质对小球藻(<i>Chlorella sorokiniana</i>)生长和代谢机制的调控

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Abstract In order to evaluate effects of light quality on the growth of Chlorella sorokiniana and metabolic mechanisms, this study was designed to monitor the growth of Chlorella sorokiniana under different light qualities with transcriptome sequencing. The results show that red and blue lights were effective for Chlorella sorokiniana growth, and the algal cell density was 52.96% and 61.11% higher than that of the white light control group after 7days of cultivation with red and blue light culture, respectively. Chlorella sorokiniana demonstrated unique physiological characteristics under different light qualities, and the blue light group had the highest concentration of Chl a, Chl b and Car with (17.84±0.26), (8.39±0.19) and (6.04±0.08)mg/L, respectively; the carbohydrate and lipid contents of the microalgae were the highest after 7 days of red light incubation, reaching (115.60±1.81)μg/mg and (18.64±0.54)%, respectively. Gene expression of Chlorella sorokiniana differed under different light qualities as indicated by transcriptome sequencing. The gene expression of carbonic anhydrase, acetyl coenzyme A carboxylase and fatty acid synthase were most significant under red light, and majority of the differentially expressed genes were involved in fatty acid synthesis and carbon fixation; the gene expression of RubisCO enzyme was the highest under blue light and the differentially expressed genes enriched in the photosynthetic system were up-regulated, and the photosynthetic rate and carbon fixation rate were the fastest; the gene expression of most of the genes in microalgae under green light was low with poor metabolic potential. The TCA cycle of Chlorella sorokiniana was active under white light, which was not conducive to the storage of carbohydrates and lipids.

Key words： light quality Chlorella sorokiniana growth metabolism gene high-throughput sequencing

Received: 14 February 2022

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	Articles by authors
	GAN Yu-hua
	WEI Qun
	MA Xiang-meng
	LI Zhong-tang
	JIN Yuan-rong
	LI Shu-yuan

Cite this article:

GAN Yu-hua,WEI Qun,MA Xiang-meng等. Regulation of light quality on Chlorella sorokiniana growth and metabolism[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4296-4303.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I9/4296

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