铜对蚯蚓转化牛粪过程抗生素抗性基因的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1117 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In this study, Eisenia fetida was used as the experimental earthworm, different Cu concentration gradients were set to study the effect of Cu on ARGs growth during earthworm conversion of cow dung. Results revealed that, under different Cu concentrations, the total abundance of ARGs showed a trend of CF (without Cu addition) -1 to 4.33×10^-1 copies/16S copies. The addition of low and medium concentrations of Cu significantly increased the total abundance of ARGs in the samples (P<0.05). By studying the changes of different ARG subtypes in different time stages, we found that the low and high concentration of Cu treatment groups (T1, T3) increased the abundances of tet-ARGs, erm-ARGs and str-ARGs during earthworm conversion, while the abundances of tet-ARGs and str-ARGs decreased in the medium-concentration Cu treatment group (T2). Redundancy analysis (RDA) showed that pH significantly affected the relative abundance of ARGs during earthworm conversion (P<0.05), and TOC, TN, and Cu also played an important role in ARGs variation. Earthworm conversion can accelerate the reduction of ARGs in cow dung and reduce its environmental risk to a certain extent, but the residual Cu in cow dung would lead to the enrichment of some ARGs during earthworm conversion of cow dung, thus it was urgent to further optimize the treatment process of earthworm conversion.

Key words： copper earthworm cow dung conversion process antibiotic resistance genes

Received: 23 March 2022

PACS:

X750

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	TIAN Xue-li
	LI Zhong-han
	YANG Feng-xia
	HAN Bing-jun
	ZHANG Ke-qiang

Cite this article:

TIAN Xue-li,LI Zhong-han,YANG Feng-xia等. Effects of copper on antibiotic resistance genes during earthworm conversion of cow dung[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(10): 4688-4695.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I10/4688

[1]	Tian X L, Han B J, Liang J F, et al.Tracking antibiotic resistance genes (ARGs) during earthworm conversion of cow dung in northern China[J].Ecotoxicology and Environmental Safety, 2021,222:112538.
[2]	吴浩玮,孙小淇,梁博文,等.我国畜禽粪便污染现状及处理与资源化利用分析[J].农业环境科学学报, 2020,39(6):1168-1176.WU H W, Sun X Q, Liang B W, et al.Analysis of livestock and poultry manure pollution in China and its treatment and resource utilization[J].Journal of Agro-Environment Science, 2020,39(6):1168-1176.
[3]	Gong X, Li S, Carson M A, et al.Spent mushroom substrate and cattle manure amendments enhance the transformation of garden waste into vermicomposts using the earthworm Eisenia fetida [J].Journal of Environmental Management, 2019,248:109263.
[4]	Shi Y P, Huang J F, Ni X W, et al.Sources and accumulation risk of heavy metal in major animal manure[J].Animal Husbandry& Feed Science, 2016,8(6):318-322,326.
[5]	Cang L, Wang Y J, Zhou D M, et al.Heavy metals pollution in poultry and livestock feeds and manures under intensive farming in Jiangsu Province, China[J].Journal of Environmental Sciences, 2004,16(3):371-374.
[6]	Ji X, Shen Q, Liu F, et al.Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai; China[J].Journal of hazardous materials, 2012,235:178-185.
[7]	唐伟欣,孙兴滨,高浩泽,等.规模化畜禽养殖场粪便中多重耐药菌分离鉴定及其耐药特征[J].农业环境科学学报, 2020,39(1):207-216.Tang W X, Sun X B, Gao H Z, et al.Isolation and identification of multidrug-resistant bacteria in feces from large-scale livestock and poultry farms and their antibiotic-resistance characteristics[J].Journal of Agro-Environment Science, 2020,39(1):207-216.
[8]	谷艳茹,韩秉君,黄继元,等.天津市家庭农场养殖粪污耐药基因赋存特征及风险评估[J].农业环境科学学报, 2020,39(2):394-402.Gu Y R, Han B J, Huang J Y, et al.Occurrence characteristics and risk assessment of resistance genes in livestock waste from family farms in Tianjin City, China[J].Journal of Agro-Environment Science, 2020, 39(2):394-402.
[9]	De la Iglesia R, Valenzuela-Heredia D, Pavissich J P, et al.Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA[J].Letters in Applied Microbiology, 2010,50(6):552-562.
[10]	Wang R, Chen M, Feng F, et al.Effects of chlortetracycline and copper on tetracyclines and copper resistance genes and microbial community during swine manure anaerobic digestion[J].Bioresource Technology, 2017,238:57-69.
[11]	Stepanauskas R, Glenn T C, Jagoe C H, et al.Coselection for microbial resistance to metals and antibiotics in freshwater microcosms[J].Environmental Microbiology, 2006,8(9):1510-1514.
[12]	田雪力,翟中葳,丁飞飞,等.奶牛场粪污制备卧床垫料过程中物料性质及污染物含量的周年变化规律[J].农业环境科学学报, 2018, 37(3):552-558.Tian XL, Zhai Z W, Ding F F, et al.Annual variation of material properties and pollutant content in the process of dairy manure making bedding in dairy farm[J].Journal of Agro-Environment Science, 2018, 37(3):552-558.
[13]	Yang F X, Zhang K Q, Zhi S L, et al.High prevalence and dissemination of β-lactamase genes in swine farms in northern China[J].Science of The Total Environment, 2019,651:2507-2513.
[14]	张俊华,陈睿华,刘吉利,等.宁夏养牛场粪污和周边土壤中抗生素及抗生素抗性基因分布特征[J].环境科学, 2021,42(6):2981-2991.Zhang J H, Chen R H, Liu J L, et al.Distribution characteristics of antibiotics and antibiotic resistance genes in manure and surrounding soil of cattle farms in Ningxia[J].Environmental Science, 2021,42(6):2981-2991.
[15]	Jia L, Lya C, Lan Z B, et al.Antibiotics in soil and water in China-a systematic review and source analysis[J].Environmental Pollution, 2020,266:115-147.
[16]	Pu C J, Yu Y, Diao J X, et al.Exploring the persistence and spreading of antibiotic resistance from manure to bio compost, soils and vegetables[J].Science of the Total Environment, 2019,688:262-269.
[17]	Ho Y B, Zakaria M P, Latif P A, et al.Degradation of veterinary antibiotics and hormone during broiler manure composting[J].Bioresource Technology, 2013,131:476-484.
[18]	Warburton P J, Amodeo N, Roberts A P.Mosaic Tetracycline Resistance Genes Encoding Ribosomal Protection Proteins[J].The Journal of antimicrobial chemotherapy, 2016,71:3333-3339.
[19]	Ding J, Zhu D, Hong B, et al.Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota[J].Environment International, 2019,124:145-152.
[20]	Thomas J C T, Oladeinde A, Kieran T J, et al.Co-occurrence of antibiotic, biocide, and heavy metal resistance genes in bacteria from metal and radionuclide contaminated soils at the Savannah River Site[J].Microbial Biotechnology, 2020,13:1179-1200.
[21]	Song W, Wang X, Gu J, et al.Effects of different swine manure to wheat straw ratios on antibiotic resistance genes and the microbial community structure during anaerobic digestion[J].Bioresource Technology, 2017,231:1-8.
[22]	钱勋.好氧堆肥对畜禽粪便中抗生素抗性基因的削减条件探索及影响机理研究[D].杨凌:西北农林科技大学, 2016.Qian X.Mechanism and conditions for reducing antibiotic resistance genes during aerobic composting of livestock manure[D].Yangling:Northwest A & F University, 2016.
[23]	Li Y, Liu B, Zhang X, et al.Effects of Cu exposure on enzyme activities and selection for microbial tolerances during swine-manure composting[J].Journal of Hazardous Material, 2015,283:512-518.
[24]	Peng S, Li H, Song D, et al.Influence of zeolite and superphosphate as additives on antibiotic resistance genes and bacterial communities during factory-scale chicken manure composting[J].Bioresource Technology, 2018,263:393-401.
[25]	张佳奇,徐艳,罗义,等.重金属协同选择环境细菌抗生素抗性及其机制研究进展[J].农业环境科学学报, 2016,35(3):409-418.Zhang J Q, Xu Y, Luo Y, et al.Co-selection mechanisms of bacterial resistance to heavy metals and antibiotics[J].Journal of Agro-Environment Science, 2016,35(3):409-418.
[26]	He L Y, Liu Y S, Su H C, et al.Dissemination of antibiotic resistance genes in representative broiler feedlots environments:identification of indicator ARGs and correlations with environmental variables[J].Environmental Science and Technology, 2014,48:13120-13129.
[27]	Hardoim P R, Overbeek L S, Elsas J D V, et al.Properties of bacterial endophytes and their proposed role in plant growth[J].Trends in Microbiology, 2008,16(10):463-471.
[28]	Bulgarelli D, Rott M, Schlaeppi K, et al.Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J].Nature, 2012,488:91-95.
[29]	张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J].中国环境科学, 2018,38(7):2609-2617.Zhang N, Li M, Liu X.Distribution and transformation of antibiotic resistance genes in Soil[J].China Environmental Science, 2018,38(7):2609-2617.
[30]	Qian X, Gu J, Sun W, et al.Diversity, abundance, and persistence of antibiotic resistance genes in various types of animal manure following industrial composting[J].Journal of Hazardous Material, 2018,344:716-722.
[31]	支苏丽,周婧,赵润,等.畜禽粪便厌氧发酵过程抗生素抗性基因归趋及驱动因子分析[J].农业工程学报, 2019,35(1):195-205.Zhi S L, Zhou J, Zhao R, et al.Analysis of antibiotic resistance genes fate and its drivers during anaerobic digestion of animal manure[J].Transactions of the Chinese Society of Agricultural Engineering, 2019, 35(1):195-205.