好氧颗粒污泥耐受PFOS的结构稳定性及微生物响应

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Abstract The aerobic granular sludge that can tolerate perfluorooctane sulfonate (PFOS) was successfully cultivated in a lab-scale sequence batch reactor system. The tolerance mechanism of aerobic granular sludge to PFOS was discovered by examining the sludge characteristics, microbial communities, microbial phenotypes, and functional pathways in various domestication periods. In the presence of PFOS, the granular sludge maintained good stability through the disintegration, formation and maturation phases. PFOS reduced the filamentous bacteria, but promoted EPS secretion. The aforementioned phenomena were beneficial for the formation of compact structure of granules. During the whole domestication stage, a significant number of resistant bacteria and bacteria associated with the maintenance of granule stability emerged in granules, mainly including unclassified_f__ Comamonadaceae, Defluviicoccus, Dongia, Rhodoplanes, Flavobacterium, Thauera, Azospira, Azoarcus, Candidatus_ Competibacter, and norank_f__A4b, and there were significant positive correlations among some genera. In addition, the abundance of genes related to the quorum sensing pathway and the bacterial chemotaxis pathway up-regulated in the disintegration period, but returned to the initial level in the formation and maturation periods. It suggested that the bacterial quorum sensing system and chemotactic ability could play important roles in the process of granular stress. Therefore, aerobic granular sludge could tolerate PFOS in various ways such as positive response of specific bacterial communities, promotion of bacterial chemotaxis and quorum sensing, enhancement of EPS secretion, and enhancement of antioxidant stress capacity of the system. The application of EPS-based aerobic granular sludge, microbial agents, and signal molecules was conducive to the treatment of wastewater containing PFOS.

Key words： perfluorooctane sulfonate aerobic granular sludge domestication microbial community tolerance

Received: 07 April 2022

PACS:

X703.1

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	Articles by authors
	XU Jia-jie
	ZHANG Ni
	XIE Zhou-yun
	XU Yi-yi
	TANG Li
	XIA Jing-fen
	YANG Guo-jing

Cite this article:

XU Jia-jie,ZHANG Ni,XIE Zhou-yun等. Structural stability and microbial response mechanism of aerobic granular sludge exposed to perfluorooctane sulfonate[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5117-5127.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I11/5117

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