包埋硫铁生物填料的制备及自养反硝化性能

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Abstract The embedded sulfur/iron biological filler (ESI Filler) was prepared by embedding and immobilization technology, and dynamic experimental research was carried out based on the upflow autotrophic denitrification reactor. By changing hydraulic retention time (HRT), pH, dissolved oxygen (DO) and other operating conditions, the denitrification performance and microbial community structure composition of ESI Filler reactor were explored. When the influent nitrate nitrogen (NO₃^--N) concentration was 30mg/L and the HRT was 10h, the NO₃^--N removal rate increased continuously to 99.80%. When the HRT was shortened to 2.5h, the removal rate of NO₃^--N decreased to about 61.35%. ESI Filler reactor had high stability to the changes of pH and DO, and the average removal rate of NO₃^--N could be maintained above 82.5%. However, the tolerance to low temperature was poor. When the temperature was reduced from 35℃ to 15℃, the average removal rate of NO₃^--N decreased from 90.12% to 68.80%. After running for 164days, the spheres did not break and scatter, showing a long service life. It was found by scanning electron microscopy that the surface of the filler was loose and porous, and a large number of rod-shaped bacteria were attached, which had become a good carrier of microorganisms. The results of high-throughput sequencing showed that the dominant bacterial genus in the embedded granules was the typical autotrophic denitrifying bacteria Thiobacillus, with an abundance of 80.79%.

Key words： sulfur autotrophic denitrification iron autotrophic denitrification embedding filler denitrification stability microbial properties

Received: 06 April 2022

PACS:

X703.1

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	Articles by authors
	LIU Yan-fang
	LIU Xiao-shuai
	YIN Si-jie
	GAO Wei
	ZHANG Miao-yu
	HAN Yan-he
	LI Zai-xing

Cite this article:

LIU Yan-fang,LIU Xiao-shuai,YIN Si-jie等. Preparation and autotrophic denitrification properties of embedded sulfur/iron biological filler[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5136-5143.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I11/5136

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