水位波动和氮浓度变化对氮转化功能基因丰度的影响

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Abstract To explore the effects of nitrogen concentration in shallow groundwater and its water table fluctuations on the abundance of soil nitrogen-transforming functional genes, taking the undisturbed soil profile from cropland around Erhai as the object, changes in nitrogen concentrations and abundance of nitrogen-transforming functional genes in soil profiles under shallow groundwater table fluctuations (SND) and continuous flooding (SNF) with conventional nitrogen concentration, and shallow groundwater table fluctuations (0ND) without nitrogen were studied. The relationship between soil environmental factors and abundance of functional genes was discussed. The results indicated that, compared with the nitrogen concentrations in soil profile before the microcosmic experiment, the total dissolved nitrogen (TDN) concentrations in SNF, SND and 0ND decreased by 44%, 21% and 30%, and NO₃⁻-N concentrations decreased by 55%, 28% and 38%, respectively. Meanwhile, compared with the abundance of nitrogen-transforming functional genes in soil profile in SND, the denitrification function gene abundances in 0ND and SNF decreased by 20% and 1%, while the anammox function gene abundances increased by 68% and 7%, and the nitrification function gene abundances decreased by 34% and increased by 23%, respectively. Changes in functional gene abundances were mainly driven by soil moisture content (MC), NH₄⁺-N, NO₃⁻-N and TDN. In conclusion, continuous flooding in soil profiles would significantly reduce dissolved nitrogen concentrations, and changes in alternation of drying-flooding and nitrogen concentrations in soil profile caused by the nitrogen concentrations in shallow groundwater and its water table fluctuations were the main drivers for changes in the abundance of nitrogen-transforming functional genes.

Key words： nitrogen-transforming functional gene soil profile alternation of drying-flooding shallow groundwater table fluctuation

Received: 15 April 2022

PACS:	X172
	X523

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	Articles by authors
	CUI Rong-yang
	LIU Gang-cai
	HU Wan-li
	FU Bin
	CHEN An-qiang

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CUI Rong-yang,LIU Gang-cai,HU Wan-li等. Effects of water table fluctuations and nitrogen concentration variations on the abundances of nitrogen-transforming functional genes in soil profiles[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5378-5386.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I11/5378

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