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| Effect of long-term low temperature on nitrogen removal and microbial community of flocculent-granular coupling CANON |
| LI Bo-lin, XIA Kai, JIANG Yu-qing, WANG Jing, ZHAO Wan-qing, HE Xiao-man |
| Hubei Key Laboratory of Mineral Resources Processing and Environment, School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China |
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Abstract The effects of long-term low temperature on nitrogen removal efficiency and microbial community structure of CANON system with equal sludge concentration ratio of flocculent-granular(1:1~1:1.5) in SBR reactor were studied in this study. The results showed that after long-term operation at low temperature(10℃) followed by recovery from low temperature(10→30℃), the removal efficiencies of NH4+-N、TN and NRR in equal proportion sludge CANON system were positively correlated with temperature, and reached 72.4%, 63.0% and 0.094kgN/(m3·d), respectively, after 30days of operation at 30℃. The activities of AOB and AAOB showed positive correlation with temperature, and reached 0.025 and 0.049gN/(gVSS·d) after recovery from low temperature, about 83.3% and 79.1% of those before cooling, respectively. NOB remained at a low level (0.001gN/(gVSS·d)). The results of high-throughput sequencing showed that long-term low-temperature had no effect on the dominant taxa at phylum level. The dominant phyla of granular and flocculent sludge after rewarming were Ignavibacterium and Proteobacteria respectively. After recovery, the relative abundances of functional bacteria Candidatus brocadia and Candidatus kuenenia (AAOB) in granular sludge were 6.017%, 2.623% respectively, and their relative abundances in flocculent sludge were 3.018% and 0.674% respectively. While Nitrosomonas (AOB) mainly distributed in flocculent sludge, and its relative abundance after recovery was 2.50%. The relative abundances of three functional bacteria were higher than the level before cooling. The equal proportion flocculent-granular sludge CANON system can effectively resist long-term low temperature.
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Received: 21 May 2022
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