地下水中两种典型抗生素复合对反硝化作用的影响

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Abstract Ciprofloxacin (CIP) and lomefloxacin (LOM) were selected as typical fluoroquinolone antibiotics, sodium acetate was used as the carbon source, and batch experiments were carried out to explore the effects of CIP or/and LOM on denitrification process. Then the influencing mechanisms were investigated by measuring the changes of indicators, such as the number and activity of denitrifying bacteria and the denitrifying enzyme activity. The results showed that compared with the blank system, LOM (100μg/L) group, CIP (100μg/L) group and LOM (50μg/L)+CIP(50μg/L) group had inhibitory effects on degradation both NO₃^--N and NO₂^--N. The inhibition rates followed an order of LOM + CIP > CIP > LOM. Antibiotic exposure could inhibit the quantity and activity of microorganisms and the activity of key denitrifying enzymes to varying degrees. The NO₃^--N degradation was affected by the activity of denitrifying bacteria and the activity of nitrate reductase (NaR), while the NO₂^--N degradation was mainly affected by the activity of nitrite reductase (NiR). The results of the combined effects of antibiotics showed that the inhibition rates of LOM + CIP group in NO₃^--N and NO₂^--N degradation stages were greater than those of single-antibiotic groups, indicating the synergistic inhibition of LOM and CIP; the synergistic inhibition of two typical antibiotics on the activities of key denitrifying enzymes was also observed; however, the inhibition of the combined antibiotics group on the quantity and activity of microorganisms indicated the antagonistic effects. The effects of different antibiotics on denitrification effects were different. LOM mainly affected on the NO₃^--N degradation stage, while the CIP or LOM+CIP showed similar inhibition effects on both stages of NO₃^--N degradation and NO₂^--N degradation.

Key words： groundwater nitrate denitrification lomefloxacin ciprofloxacin

Received: 05 May 2022

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X523

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	LI Wei
	HE Jiang-tao
	DENG Lu

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LI Wei,HE Jiang-tao,DENG Lu. Effects of the combination of two typical antibiotics on denitrification process in groundwater[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(12): 5703-5712.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I12/5703

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