|
|
Enrichment of antibiotics in cherry radish and its potential risk |
LI Yang-yang, MEI Qin-yuan, LIU Zi-yin, YIN Li-chun, WANG Xing-shuo, CHEN Ze-you |
Department of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
|
|
Abstract To explore the potential ecological and health risks of soil antibiotic pollution, a greenhouse pot experiment on cherry radishes was conducted from June to September 2021. Oxytetracycline (OTC) or streptomycin (STR) contaminated water was applied to soil every 6days, and the biomass, plant uptake of antibiotics, and the antibiotic resistance characteristics of the microbiome of cherry radish were analyzed. In addition, the potential human health risks of residual antibiotic and antibiotic-resistant bacteria (ARB) in radish plants were evaluated by risk quotient, calculation of intake index, and the mouse feeding tests. The results showed that continuous application of OTC significantly promoted the growth of radish plants and increased the total biomass of radish plants by 23.1% on 74 days compared with the non-antibiotic applied control group, while continuous application of STR had no significant effect on the growth of radish plants. Radish plants accumulated a certain amount of the applied two antibiotics, and the residual amount of STR in tissues was higher than OTC by 1~2 orders of magnitude. Antibiotics were mainly enriched in leaves at the early growth stage, and antibiotic residue in the neck of fleshy roots at the later growth stage was significantly increased (P < 0.05). The proportion of culturable OTC- and STR-resistant bacteria in total culturable bacteria increased by 2.48×10-6%~5.05 ×10-4% and 0.19%~3.32%, respectively. Consumption of the neck of fleshy roots exposed to antibiotics could increase the body's intake of antibiotics and ARB, but the associated health risk was relatively low, as the assessed risk index HQ was less than 0.1 and the antibiotic residue in feces of mice eating radish plants was below the detection limit.
|
Received: 06 June 2022
|
|
|
|
|
[1] |
刘鹏霄,王旭,冯玲.自然水环境中抗生素的污染现状、来源及危害研究进展[J]. 环境工程, 2020,38(5):36-42. Liu P X, Wang X, Feng L. Occurrences, resources and risk of antibiotics in aquatic environment:A review[J]. Environmental Engineering, 2020,38(5):36-42.
|
[2] |
Qian M R, Wu H Z, Wang J M, et al. Occurrence of trace elements and antibiotics in manure- based fertilizers from the Zhejiang Province of China[J]. Science of the Total Environment, 2016,559:174-181.
|
[3] |
张慧敏,章明奎,顾国平.浙北地区畜禽粪便和农田土壤中四环素类抗生素残留[J]. 生态与农村环境学报, 2008,24(3):69-73. Zhang H M, Zhang M K, Gu G P. Residues of tetracyclines in livestock and poultry manures and agricultural soils from North Zhejiang Province[J]. Journal of Ecology and Rural Environment, 2008,24(3):69-73.
|
[4] |
萨仁其其格,赵文岩,马静静.HPLC法检测内蒙古牧区土壤中链霉素残留[J]. 分析化学, 2009,37(A2):217. Sarenqiqige, Zhao W Y, Ma J J. Determination of streptomycin residues in soil of pastoral area of Inner Mongolia by HPLC[J]. Chinese Journal of Analytical Chemistry, 2009,37(A2):217.
|
[5] |
王冲,罗义,毛大庆.土壤环境中抗生素的来源、转归、生态风险以及消减对策[J]. 环境化学, 2014,33(1):19-29. Wang C, Luo Y, Mao D Q. Sources, fate, ecological risks and mitigation strategies of antibiotics in the soil environment[J]. Environmental Chemistry, 2014,33(1):19-29.
|
[6] |
Pan M, Chu L M. Phytotoxicity of veterinary antibiotics to seed germination and root elongation of crops[J]. Ecotoxicology and Environmental Safety, 2016,126:228-237.
|
[7] |
秦俊梅,熊华烨,李兆君.施用含四环素类抗生素鸡粪对玉米生长的影响及其残留特征[J]. 灌溉排水学报, 2018,37(9):22-28. Qin J M, Xiong H Y, Li Z J. Decay of tetracycline antibiotics and the growth of maize in soil amended with chicken manure[J]. Journal of Irrigation and Drainage, 2018,37(9):22-28.
|
[8] |
黄佳,杨甜,李泽林,等.不同浓度四环素和土霉素对生菜生长的影响及生态毒性[J]. 应用与环境生物学报, 2022,28(4):995-1001. Huang J, Yang T, Li Z L, et al. Effect of different concentrations of tetracycline and oxytetracycline on the growth and ecotoxicity in lettuce[J]. Chinese Journal of Applied and Environmental, 2022,28(4):995-1001.
|
[9] |
张继旭,申国明,孔凡玉,等.四环素对烤烟生长发育及光合作用的影响研究[J]. 农业环境科学学报, 2017,36(1):48-56. Zhang J X, Shen G M, Kong F Y, et al. Effect of tetracycline on the growth and photosynthesis of flue-cured tobacco[J]. Journal of Agro-Environment Science, 2017,36(1):48-56.
|
[10] |
黄丹,叶茂,朱国繁,等.抗生素/抗性细菌/抗性基因在土壤-植物系统中迁移转化及阻控消减的研究进展[J]. 土壤, 2020,52(5):891-900. Huang D, Ye M, Zhu G F, et al. Migration and risk control of antibiotic and antibiotic resistance bacteria/genes in soil-plant system:a review[J]. Soils, 2020,52(5):891-900.
|
[11] |
王盼亮,张昊,王瑞飞,等.抗生素暴露对小白菜幼苗生长及内生细菌的影响[J]. 农业环境科学学报, 2017,36(9):1734-1740. Wang P L, Zhang H, Wang R F, et al. Effects of antibiotic exposure on the growth and endophytic bacterial community of Chinese cabbage seedlings[J]. Journal of Agro-Environment Science, 2017,36(9):1734-1740.
|
[12] |
于晓雯,索全义,史李萍.外源四环素类抗生素对土壤微生物多样性的影响[J]. 北方园艺, 2021,(2):91-96. Yu X W, Suo Q Y, Shi L P. Effects of exogenous tetracycline antibiotics on soil microbial diversity[J]. Northern Horticulture, 2021,(2):91-96.
|
[13] |
肖磊,王海芳.四环素类抗生素在土壤环境中的残留及环境行为研究进展[J]. 应用化工, 2020,49(12):3178-3184. Xiao L, Wang H F. Tetracycline residues and environmental behavior of tetracycline antibiotics in soil:A review[J]. Applied Chemical Industry, 2020,49(12):3178-3184.
|
[14] |
Chuang Y H, Zhang Y J, Zhang W, et al. Comparison of accelerated solvent extraction and quick, easy, cheap, effective, rugged and safe method for extraction and determination of pharmaceuticals in vegetables[J]. Journal of Chromatography A, 2015,1404:1-9.
|
[15] |
叶倩,黄健祥,孙玲,等.QuEChERS-超高效液相色谱-串联质谱法测定叶类蔬菜中5种植物生长调节剂的残留[J]. 农药学学报, 2017,19(5):589-596. Ye Q, Huang J X, Sun L, et al. Determination of five plant growth regulator residues in leafy vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2017,19(5):589-596.
|
[16] |
Spoerri A S, Jan P, Cognard E, et al. Comprehensive screening of veterinary drugs in honey by ultra-high-performance liquid chromatography coupled to mass spectrometry[J]. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment, 2014,31(5):806-816.
|
[17] |
Hanna N, Sun P, Sun Q, et al. Presence of antibiotic residues in various environmental compartments of Shandong province in eastern China:Its potential for resistance development and ecological and human risk[J]. Environment International, 2018,114:131-142.
|
[18] |
Prosser R S, Sibley P K. Human health risk assessment of pharmaceuticals and personal care products in plant tissue due to biosolids and manure amendments, and wastewater irrigation[J]. Environment International, 2015,75:223-233.
|
[19] |
Liu X J, Liang C Z, Liu X H, et al. Occurrence and human health risk assessment of pharmaceuticals and personal care products in real agricultural systems with long-term reclaimed wastewater irrigation in Beijing, China[J]. Ecotoxicology and Environmental Safety, 2020, 190:110022.
|
[20] |
国家体育总局.2014年国民体质监测公报[EB/OL]. http://www.sport.gov.cn/n16/n1077/n1227/7328132.html,2015-11-25/2022-06-06. General Administration of Sport of China. Bulletin of National Physical Fitness Monitoring in 2014[EB/OL]. http://www.sport.gov.cn/n16/n1077/n1227/7328132.html,2015-11-25/2022-06-06.
|
[21] |
迟荪琳,王卫中,徐卫红,等.四环素类抗生素对不同蔬菜生长的影响及其富集转运特征[J]. 环境科学, 2018,39(2):935-943. Chi S L, Wang W Z, Xu W H, et al. Effects of tetracycline antibiotics on growth and characteristics of enrichment and transformation in two vegetables[J]. Environmental Science, 2018,39(2):935-943.
|
[22] |
鲍陈燕,顾国平,章明奎.兽用抗生素胁迫对水芹生长及其抗生素积累的影响[J]. 土壤通报, 2016,47(1):164-172. Bao C Y, Gu G P, Zhang M K. Effects of veterinary antibiotic stress on growth and antibiotic accumulation of oenanthe javanica DC[J]. Chinese Journal of Soil Science, 2016,47(1):164-172.
|
[23] |
Khan K Y, Ali B, Zhang S, et al. Phytotoxic effects on chloroplast and UHPLC-HRMS based untargeted metabolomic responses in Allium tuberosum Rottler ex Sprengel (Chinese leek) exposed to antibiotics[J]. Ecotoxicology and environmental safety, 2022,234:113418-113418.
|
[24] |
王晓洁,赵蔚,张志超,等.兽用抗生素在土壤中的环境行为、生态毒性及危害调控[J]. 中国科学:技术科学, 2021,51(6):615-636. Wang X J, Zhao W, Zhang Z C, et al. Veterinary antibiotics in soil:environmental processes, ecotoxicity and risk mitigation[J]. Scientia Sinica(Technologica), 2021,51(6):615-636.
|
[25] |
李伟明,鲍艳宇,周启星.四环素类抗生素降解途径及其主要降解产物研究进展[J]. 应用生态学报, 2012,23(8):2300-2308. Li W M, Bao Y Y, Zhou Q X. Degradation pathways and main degradation products of tetracycline antibiotics:Research progress[J]. Chinese Journal of Applied Ecology, 2012,23(8):2300-2308.
|
[26] |
Aks A, Mtm B, Ab C. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment-ScienceDirect[J]. Chemosphere, 2006,65(5):725-759.
|
[27] |
饶怡璇,汪斌,何玉洁,等.抗生素在土壤中的归趋及不可提取态残留[J]. 环境化学, 2022,41(2):549-560. Rao Y X, Wang B, He Y J, et al. Fate and non-extractable residues of antibiotics in soil[J]. Environmental Chemistry, 2022,41(2):549-560.
|
[28] |
章程.典型抗生素在土壤-植物中的迁移及其机制[D]. 北京:中国农业科学院, 2018. Zhang C. The fate of veterinary antibiotics in system of soil and plants and their mechanism[D]. Beijing:Chinese Academy of Agricultural Sciences, 2018.
|
[29] |
贺德春,吴根义,许振成,等.小白菜和白萝卜对四环素类抗生素的吸收累积特征[J]. 农业环境科学学报, 2014,33(6):1095-1099. He D C, Wu G Y, Xu Z C, et al. Uptake of selected tetracycline antibiotics by pakchoi and radish from manure-amended soils[J]. Journal of Agro-Environment Science, 2014,33(6):1095-1099.
|
[30] |
李蓝青.肥料中四环素类抗生素的检测方法及其在土壤中的降解与作物吸收效应[D]. 上海:上海交通大学, 2016. Li L Q. Determination of tetracyclines in fertilizer and their degradation in soil and accumulation by crop[D]. Shanghai:Shanghai Jiao Tong University, 2016.
|
[31] |
朱昶.四环素和土霉素的光降解产物与土壤酶相互作用的研究[D]. 青岛:山东大学, 2020. Zhu X. Study on the interaction of photolysis products of tetracycline and oxytetracycline with soil enzymes[D]. Qingdao:Shandong University, 2020.
|
[32] |
陈龙,梁子宁,朱华.植物内生菌研究进展[J]. 生物技术通报, 2015,31(8):30-34. Chen L, Liang Z N, Zhu H. Research advances in the atudies of plant entophytic[J]. Biotechnology Bulletin, 2015,31(8):30-34.
|
[33] |
Zhang H, Li X N, Yang Q X, et al. Plant growth, antibiotic uptake, and prevalence of antibiotic resistance in an endophytic system of pakchoi under antibiotic exposure[J]. International Journal of Environmental Research and Public Health, 2017,14(11):1136.
|
[34] |
Yashiro E, Mcmanus P S. Effect of Streptomycin Treatment on Bacterial Community Structure in the Apple Phyllosphere[J]. Plos One, 2012,7(5):e37131.
|
[35] |
Wang Y, Lu J, Mao L K, et al. Antiepileptic drug carbamazepine promotes horizontal transfer of plasmid-borne multi-antibiotic resistance genes within and across bacterial genera[J]. Isme Journal, 2019,13(2):509-522.
|
[36] |
Stockwell V O, Duffy B. Use of antibiotics in plant agriculture[J]. Revue Scientifique Et Technique-Office International Des Epizooties, 2012,31(1):199-210.
|
[37] |
明德松,潘艳萍,朱炎.鲁氏不动杆菌的临床分布及耐药性分析[J]. 中华医院感染学杂志, 2014,24(10):2351-2352,2355. Ming D X, Pan Y P, Zhu Y. Clinical distribution and drug resistance of Acinetobacter lwoffii[J]. Chinese Journal of Nosocomiology, 2014, 24(10):2351-2352,2355.
|
[38] |
Chen Z Y, Zhang Y J, Gao Y Z, et al. Influence of dissolved organic matter on tetracycline bioavailability to an antibiotic-resistant bacterium[J]. Environmental Science & Technology, 2015,49(18):10903-10910.
|
[39] |
Zhang Y J, Hu H W, Chen Q L, et al. Transfer of antibiotic resistance from manure-amended soils to vegetable microbiomes[J]. Environment International, 2019,130:104912.
|
[40] |
Mahmoud M a M, Abdel-Mohsein H S. Hysterical tetracycline in intensive poultry farms accountable for substantial gene resistance, health and ecological risk in Egypt-manure and fish[J]. Environmental Pollution, 2019,255:113039.
|
[41] |
Ahmed M B M, Rajapaksha A U, Lim J E, et al. Distribution and accumulative pattern of tetracyclines and sulfonamides in edible vegetables of cucumber, tomato, and lettuce[J]. Journal of Agricultural and Food Chemistry, 2015,63(2):398-405.
|
[42] |
王卫中,迟荪琳,徐卫红.四环素类抗生素对土壤-生菜系统的生物效应及其迁移降解特性[J]. 环境科学, 2021,42(3):1545-1558. Wang W Z, Chi S L, Xu W H. Biological effect of tetracycline antibiotics on a soil-lettuce system and its migration degradation characteristics[J]. Environmental Science, 2021,42(3):1545-1558.
|
|
|
|