典型抗生素对湿地反硝化甲烷厌氧氧化的影响

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Abstract The Baiyangdian wetland, with a high methane emission flux and heavily contaminated with quinolone antibiotics (QNs), was selected as the study area. The short-term effects of typical QNs including Ofloxacin (OFL), Norfloxacin (NOR), and Flumequine (FLU) on denitrifying anaerobic methane oxidation (DAMO) processes in Baiyangdian wetland was investigated by conducting ¹³C-labeled microcosm experiments. The results showed that: All three QNs promoted the microbial DAMO process, and the promotion effect on DAMO was OFL > NOR > FLU; Except for 2ng/g OFL, which had a negligible effect on the DAMO reaction rate, additional concentrations (20, 200, 2000ng/g) all promoted the DAMO processes in Baiyangdian wetlands, and the promotion showed a dose effect in general; Further analysis of the effects of QNs on the nitrite-DAMO driven by NO₂^- and the nitrite-DAMO driven by NO₃^- respectively, it could be found that 200ng/g NOR inhibited the bacterial-driven DAMO process with NO₂^- as electron acceptor (nitrite-DAMO), but promoted to a greater extent the archaea-driven DAMO process with NO₃^- as electron acceptor (nitrate-DAMO), ultimately showing a facilitation effect of QNs on the overall DAMO process driven by both NO₃^- and NO₂^-.

Key words： Baiyangdian wetland quinolone antibiotics methane denitrifying anaerobic methane oxidation ¹³C-labeled microcosm experiment

Received: 21 June 2022

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X172

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	Articles by authors
	YANG Yu-qi
	ZHAO Yue-wen
	ZHANG Qiu-lan
	LIU Chang-li
	WEN Meng-tuo

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YANG Yu-qi,ZHAO Yue-wen,ZHANG Qiu-lan等. Effects of typical antibiotics on denitrifying anaerobic methane oxidation in the wetland[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 631-637.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/631

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