干湿交替循环频率对生物膜特征及其微生物群落的影响

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Abstract The periphytic biofilm was sampled in natural environment and cultivated under indoor controlled conditions to clarify the biofilm resistance mechanisms under the influence of alternate drying and wetting cycles. The morphology, microbial community structure, diversity and function of natural biofilms were examined using 16S rRNA gene high throughput sequencing and confocal laser scanning microscopy. The results showed that biofilm thickness was reduced from 9.10 to 6.46 μm with the increase of alternate drying-wetting cycles from one (C1) to five (C5). The extracellular polymer content decreased from 2.64 μm³/μm³ (control) to 1.9 μm³/μm³(C1), and then increased from 4.41 μm³/μm³(C3) to 1.54 μm³/μm³(C5, P=0.004). The ratio of live/dead bacteria increased from 2.52 (C1) to 3.60 (C3), and then decreased to 0.72(C5, P=0.009). However, the changes in surface area to volume ration and roughness coefficient with the alternate drying and wetting cycles were not significant compared to the control. In contrast to the control, the richness and diversity of the microbial community decreased and the indicator species changed when biofilm exposed to alternate drying and wetting cycles. The dominant bacteria in biofilm shifted from Proteobacteria to Cyanobacteria-related flora when the alternate drying and wetting cycles reached to five (C5). Moreover, functional groups associated with photoautotrophy became the most abundant. Therefore, biofilm morphology and microbial community structure were associated with the alternate drying and wetting cycles. Microbial communities exhibited high degrees of resistance under lower alternate drying and wetting cycles, whereas showed high degrees of resilience to increased alternate drying and wetting cycles.

Key words： alternate drying-wetting cycles biofilm microbial community resistance resilience

Received: 11 July 2022

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X172

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	LI Chun-yan
	XIE Shan-shan
	WANG Wen-wen
	ZHU Shi-jun
	WEN Chen
	LI Ni-hong
	LUO Xia

Cite this article:

LI Chun-yan,XIE Shan-shan,WANG Wen-wen等. Effects of alternate drying-wetting cycles on biofilm characteristics and their microbial communities[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 854-862.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/854

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