褐菖鲉稚鱼对柴油污染的行为和生理响应

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1038 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract Oil pollution is one of the most dominant forms of pollution in China's coastal areas and has a strong toxic effect on the marine fish, whose changes in behavior and physiological features are one of the most apparent manifestations of toxic stress. In this study, the petroleum hydrocarbon concentration in the offshore marine area of Zhoushan was investigated to evaluate the effects of the water-soluble fraction of diesel oil (DWSF) on the activity, exploratory behavior, and predation ability of Sebastsicus marmoratus, a typical rockfish that inhabits the coastal waters of the East China Sea. Histological changes in gill tissue and enzyme activity related to neurotransmission were also investigated. The results show that the exploratory behavior and activity of post-larval fish first increased and then decreased with an increase in DWSF concentration. It was observed that the fish's behavioral activity was significantly inhibited, and the predation ability was impaired when the concentration of DWSF exceeded the standard of inferior Class-V water quality (DWSF>0.30mg/L). Once the concentration of DWSF reached 0.45mg/L, the gill tissue of the post-larval fish showed noticeable lesions and damage. Meanwhile, the activity of cholinesterase associated with neurotransmission was significantly inhibited, resulting in a high mortality rate. This suggests that marine diesel pollution can trigger apparent anomalous behavior, significantly reduce predation ability, and ultimately increase mortality in post-larval S. marmoratus. The impairment of the nervous and respiratory systems may be one critical reason for abnormal behaviors. Therefore, the concentration of DWSF in seawater should be controlled below 0.45mg/L in order to ensure the normal growth and development of the larvae of S. marmoratus and other near-shore resident rockfish.

Key words： Sebastiscus marmoratus behavior histology cholinesterase mortality rate

Received: 24 September 2022

PACS:

X174

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	TIAN Meng-jia
	GUO Hao-yu
	SHEN Yu-xin
	ZHANG Xiao-feng
	TIAN Kuo
	CHAI Xue-jun
	ZHANG Ding-yuan
	ZHANG Xiu-mei

Cite this article:

TIAN Meng-jia,GUO Hao-yu,SHEN Yu-xin等. Behavioral and physiological responses of post-larval Sebastiscus marmoratus to diesel pollution[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(5): 2622-2631.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I5/2622

[1]	Rodrigo A, Zoe W, Wang Z, et al. Interactions between zooplankton and crude oil:toxic effects and bioaccumulation of polycyclic aromatic hydrocarbons[J]. Plos One, 2013,8(6):e67212.
[2]	杨梦茹,徐雄,王东红,等.长江典型江段水体PAHs的分布特征、来源及其生态风险[J].中国环境科学, 2022,42(11):5308-5317. Yang M R, Xu X, Wang D H, et al. Distribution characteristics, source and ecological risks assessment of PAHs in water bodies of typical sections of the Yangtze River[J]. China Environmental Science, 2022, 42(11):5308-5317.
[3]	Olivares-Rubio H F, Espinosa-Aguirre J J. Acetylcholinesterase activity in fish species exposed to crude oil hydrocarbons:A review and new perspectives[J]. Chemosphere, 2021,264:128401.
[4]	Gercken J, Förlin L, Andersson J. Developmental disorders in larvae of eelpout (Zoarces viviparus) from German and Swedish Baltic coastal waters[J]. Marine Pollution Bulletin, 2006,53(8/9):497-507.
[5]	Halder M, Léonard M, Iguchi T, et al. Regulatory aspects on the use of fish embryos in environmental toxicology[J]. Integrated Environmental Assessment and Management, 2010,6(3):484-491.
[6]	Giacomin M, Gillis P L, Bianchini A, et al. Interactive effects of copper and dissolved organic matter on sodium uptake, copper bioaccumulation, and oxidative stress in juvenile freshwater mussels (Lampsilis siliquoidea)[J]. Aquatic Toxicology, 2013,144:105-115.
[7]	Hansen B H, Salaberria I, Read K E, et al. Developmental effects in fish embryos exposed to oil dispersions-The impact of crude oil micro-droplets[J]. Marine Environmental Research, 2019,150:104753.
[8]	Khursigara A J, Perrichon P, Bautista N M, et al. Cardiac function and survival are affected by crude oil in larval red drum, Sciaenops ocellatus[J]. Science of the Total Environment, 2017,579:797-804.
[9]	田丽娜,杨金生,周佑霖,等.原油对潮间带大弹涂鱼(Boleophthalmus pectinirostris)抗氧化酶活性影响的初步探究[J].海洋环境科学, 2022,41(1):135-141. Tian L N, Yang J S, Zhou Y L, et al. The primary study on antioxidase activities of Boleophthalmus pectinirostris exposed to crude oil in intertidal zone[J]. Marine Environmental Science, 2022,41(1):135-141.
[10]	董冉,蒋玫,李磊,等.0#柴油水溶性成分对黑鲷肝脏、鳃、肌肉组织中抗氧化酶活性的影响[J].海洋渔业, 2016,38(2):190-197. Dong R, Jiang M, Li L, et al. Effects of water-soluble fraction in No.0diesel oil exposure on the antioxidase activities in liver, gill and muscle of Sparus microcephalus [J]. Marine Fisheries, 2016,38(2):190-197.
[11]	夏斌,陈碧鹃,李传慧,等.胜利原油对半滑舌鳎肝脏超氧化物歧化酶和过氧化氢酶活性的影响[J].渔业科学进展, 2011,32(1):53-59. Xia B, Chen B J, Li C H, et al. Effect of Shengli crude oil on the activities of superoxide dismutase and catalase in the liver of tongue sole Cynoglossus semilaevis [J]. Progress in Fishery Sciences, 2011, 32(1):53-59.
[12]	Claudio L, Kwa W C, Russell A L, et al. Testing methods for developmental neurotoxicity of environmental chemicals[J]. Toxicology and Applied Pharmacology, 2000,164(1):1-14.
[13]	王麒杰,竺柏康.舟山港口石油类海洋污染的调查和防治[J].浙江海洋大学学报(自然科学版), 2013,32(2):173-177. Wang Q J, Zhu B K. Petroleum marine pollution investigation and prevention of Zhoushan port[J]. Journal of Zhejiang Ocean University (Natural Science), 2013,32(2):173-177.
[14]	Ng W C, Sadovy Y, Leung F C C. Mating system of the rockfish, Sebastiscus marmoratus as revealed by DNA fingerprinting[J]. Ichthyological Research, 2003,50(4):339-348.
[15]	曾旭,章守宇,汪振华,等.马鞍列岛褐菖鲉Sebasticus marmoratus栖息地适宜性评价[J].生态学报, 2016,36(12):3765-3774. Zeng X, Zhang S Y, Wang Z H, et al. Habitat suitability assessment of Sebasticus marmoratus in the rocky reef region of the Ma'an Archipelago[J]. Acta Ecologica Sinica, 2016,36(12):3765-3774.
[16]	孙文静,王晓艳,祁鹏志,等.苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响[J].海洋与湖沼, 2018,49(4):897-903. Sun W J, Wang X Y, Qi P Z, et al. Effects of benzo[a]pyrene on erod activity, mrna expression, and protein expression of CYP1A1 in the liver of Sebasticus marmoratus [J]. Oceanologia et Limnologia Sinica, 2018,49(4):897-903.
[17]	李瑞霞.苯并(a)芘芘菲三种多环芳烃对褐菖鲉仔鱼生长发育的影响研究[D].厦门:厦门大学, 2008. Li R X. Effects of three polycycilc aromatic hydrocarbons:benzo[a]pyrene, pyrene and phenanthrene on development of early life stages of Sebasticus marmoratus [D]. Xiamen:Xiamen University, 2008.
[18]	GB/17378.4-2007海洋监测规范[S]. GB/17378.4-2007 The specification for marine monitoring[S].
[19]	Singer M M, Aurand D, Bragin G E, et al. Standardization of the preparation and quantitation of water-accommodated fractions of petroleum for toxicity testing[J]. Marine Pollution Bulletin, 2000, 40(11):1007-1016.
[20]	帅异莹.舟山海域石油烃污染调查及相关石油烃降解微生物的应用研究[D].浙江:浙江大学, 2019. Shuai Y Y. Investigation of petroleum hydrocarbon pollution in Zhoushan sea area and the application of related oil-degrading microorganisms[D]. Zhejiang:Zhejiang University, 2019.
[21]	Shi Q P, Wang M, Shi F Q, et al. Developmental neurotoxicity of triphenyl phosphate in zebrafish larvae[J]. Aquatic Toxicology, 2018, 203:80-87.
[22]	曾令清,李梦露,夏茂芹,等.异育银鲫易钓性的表型基础和生态结果及饥饿响应[J].水生生物学报, 2018,42(4):751-761. Zeng L Q, Li M L, Xia M Q, et al. The phenotypic basis, ecological consequences and fasting responses to angling of juvenile gibel carp (carassius auratus gibelio)[J]. Acta Hydrobiologica Sinica, 2018, 42(4):751-761.
[23]	Wijayanti G E, Setyawan P, Kurniawati D I. A simple paraffin embedded protocol for fish egg, embryo, and larvae[J]. Scripta Biologica, 2017,4(2):85-89.
[24]	He W, Xia W, Cao Z D, et al. The effect of prolonged exercise training on swimming performance and the underlying biochemical mechanisms in juvenile common carp (Cyprinus carpio)[J]. Comparative Biochemistry and Physiology, Part A, 2013,166(2):308-315.
[25]	Mc A, Er A, Sb A, et al. Developmental exposure to a POPs mixture or PFOS increased body weight and reduced swimming ability but had no effect on reproduction or behavior in zebrafish adults[J]. Aquatic Toxicology, 2021,237:105882.
[26]	Oliveira M, Gravato C, Guilhermino L. Acute toxic effects of pyrene on Pomatoschistus microps(Teleostei, Gobiidae):mortality, biomarkers and swimming performance[J]. Ecological Indicators, 2012,19:206-214.
[27]	Hicken C E, Linbo T L, Baldwin D H, et al. Sublethal exposure to crude oil during embryonic development alters cardiac morphology and reduces aerobic capacity in adult fish[J]. Proceedings of the National Academy of Sciences of the United States of America, 2011,108(17):7086-7090.
[28]	娄宇栋,何娇娇,竺琰,等.3种常见养殖鱼类的游泳喜好研究[J].浙江海洋大学学报(自然科学版), 2018,37(3):228-232. Lou Y D, He J J, Zhu Y, et al. Study of three species of common fishes'swimming preferences[J]. Journal of Zhejiang Ocean University (Natural Science), 2018,37(3):228-232.
[29]	Härkönen L, Hyvärinen P, Paappanen J, et al. Explorative behavior increases vulnerability to angling in hatchery-reared brown trout (Salmo trutta)[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2014,71(12):1900-1909.
[30]	Vignet C, Menach K L, Lyphout L, et al. Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish-part II:behavior[J]. Environmental Science and Pollution Research International, 2014,21(24):13818.
[31]	Munnelly R T, Windecker C C, Reeves D B, et al. Effects of short-duration oil exposure on bay anchovy (Anchoa mitchilli) embryos and larvae:mortality, malformation, and foraging[J]. Aquatic Toxicology, 2021,237:105904.
[32]	Hunter J. Feeding ecobgy and predation of marine fish larvae[J]. Marine Fish Larvae Morphology Ecology and Relation to Fisheries, 1981,8122:34-77.
[33]	Johansen J L, Allan B, Rummer J L, et al. Oil exposure disrupts early life-history stages of coral reef fishes via behavioural impairments[J]. Nature Ecology & Evolution, 2017,1(8):1146-1152.
[34]	Snieszko S F. The effects of environmental stress on outbreaks of infectious diseases of fishes[J]. Journal of Fish Biology, 1974,6(2):197-208.
[35]	Negreiros L A, Silva B F, Paulino M G, et al. Effects of hypoxia and petroleum on the genotoxic and morphological parameters of Hippocampus reidi [J]. Comparative Biochemistry and Physiology, Part C, 2011,153:408-414.
[36]	Hassaninezhad L, Safahieh A, Salamat N, et al. Assessment of gill pathological responses in the tropical fish yellowfin seabream of Persian Gulf under mercury exposure[J]. Toxicology Reports, 2014,1:621-628.
[37]	贾晋璞,王薛平,毕春娟,等.滴水湖及其鲫鱼体内PAHs分布特征与影响因素分析[J].中国环境科学, 2015,35(11):3414-3421. Jia J P, Wang X P, Bi C J, et al. The distribution characteristics of PAHs and the influencing factors in Dishui lake and crucian carp[J]. China Environmental Science, 2015,35(11):3414-3421.
[38]	Au D. The application of histo-cytopathological biomarkers in marine pollution monitoring:a review[J]. Marine Pollution Bulletin, 2004, 48(9/10):817-834.
[39]	Brown-Petersona N J, Krasnec M, Takeshita R, et al. A multiple endpoint analysis of the effects of chronic exposure to sediment contaminated with Deepwater Horizon oil on juvenile Southern flounder and their associated microbiomes[J]. Aquatic Toxicology, 2015,165:197-209.
[40]	Majid A, Hesni A, Savari A D, et al. Gill histopathological changes in milkfish (Chanos chanos) exposed to acute toxicity of diesel oil[J]. World Applied Sciences Journal, 2011,14(10):1487-1492.
[41]	Facd A, Mgpb C, Lccm A, et al. Morphological and histopathological changes in seahorse (Hippocampus reidi) gills after exposure to the water-accommodated fraction of diesel oil[J]. Marine Pollution Bulletin, 2020,150:110769.
[42]	Agamy E. Impact of laboratory exposure to light Arabian crude oil, dispersed oil and dispersant on the gills of the juvenile brown spotted grouper (Epinephelus chlorostigma):A histopathological study[J]. Marine Environmental Research, 2013,86:46-55.
[43]	Letícia A, Dzul-Caamal R, Jaime R O, et al. Effects of polycyclic aromatic hy drocarbons in Gambusia yucatana, an endemic fish from Yucatán Peninsula, Mexico[J]. Polycyclic Aromatic Compounds, 2020,43(3):907-924.
[44]	Holth T F, Tollefsen K E. Acetylcholine esterase inhibitors in effluents from oil production platforms in the North Sea[J]. Aquatic Toxicology, 2012,112-113:92-98.
[45]	Palanikumar L, Kumaraguru A K, Ramakritinan C M, et al. Biochemical response of anthracene and benzo[a] pyrene in milkfish Chanos chanos[J]. Ecotoxicology and Environmental Safety, 2012, 75:187-197.
[46]	Kim W K, Lee S K, Choi K, et al. Integrative assessment of biomarker responses in pale chub (Zacco platypus) exposed to copper and benzo[a]pyrene[J]. Ecotoxicology and Environmental Safety, 2013, 92(1):71-78.
[47]	Drapeau P, Saint-Amant L, Buss R R, et al. Development of the locomotor network in zebrafish[J]. Progress in Neurobiology, 2002, 68(2):85-111.
[48]	Sultan D, David A H, Changiz G. Neurobiology of butyrylcholinesterase[J]. Nature Reviews Neuroscience, 2003,4(2):131-138.
[49]	Gold-Bouchot G, Zapata-Perez O, Rodriguez-Fuentes G, et al. Biomarkers and pollutants in the Nile Tilapia, Oreochromis niloticus, in four lakes from San Miguel, Chiapas, Mexico[J]. International Journal of Environment and Pollution, 2006,26(1/2/3):129-141.
[50]	Galgani F, Bocquené G, Cadiou Y. Evidence of variation in cholinesterase activity in fish along a pollution gradient in the North Sea[J]. Marine Ecology Progress Series, 1992,91(1/3):77-82.