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| Microbial resistance and community changes during the adsorption of sulfadiazine through fixed bed |
| ZENG Xue-Yu1,2, DENG De-Qi1, LIU Kang-Kang1, HU Mei-Li1, CAO Hai-Lei1, Lü Jian1 |
1. Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Jinhuang Environmental Sci-Tech Co., Ltd, Fuzhou 350002, China |
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Abstract The fixed bed was filled with phosphoric acid modified coffee residue biochar (PABC-700) for the dynamic adsorption of sulfadiazine (SDZ) in water. The effects of influent flow rate, adsorbent filling height and initial concentration on the adsorption properties of sulfadiazine were systematically analyzed, and the microbial resistance and community change in the fixed bed under the long-term selective pressure of SDZ were then evaluated. The results showed that the dynamic adsorption behavior of PABC-700fixed bed for SDZ in water conformed to Yoon-Nelson and Adams-Bohart models. The resistance gene abundance changes of sul1 and sul2 according to the results obtained from qPCR showed that the long-term operation of the fixed bed would lead to the proliferation of sulfonamides resistance genes in the effluent. With the increase of SDZ concentration in the system, the abundance of sul1 and sul2 on biochar also showed an increasing trend. The expression of sul1 and sul2 in the group exposed to 1.0mg/L sulfadiazine was 1.97 and 1.53times that of the control group. The microbial community structure on the surface of biochar, as well as the inlet and outlet of the water after the long-term operation were analyzed using Miseq PE300sequencing technology. The results showed that the microbial community diversity on the fixed bed was significantly attenuated compared with the control group, and there was a significant correlation between the purified effluent and the bacterial community structure on the fixed bed biochar. More attention should be paid to the proliferation of conditional pathogenic bacteria Mycobacterium in filtered effluent.
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Received: 02 November 2022
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