粪肥铜锌残留对土壤N<sub>2</sub>O排放及其关联因子的影响

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Abstract To explore the responses of different pH soils to Cu and Zn residues in the swine manure from intensive animal husbandry, three vegetable-planted soils with the acidic (pH 5.81), neutral (pH 7.18) and alkaline (pH 8.00) were used to treat with an addition of zero manure (CK), organic manure (M) and polluted manure (MP), respectively. All treatments were incubated for half year under the alternation of dry and wet conditions to exhaust the available carbon and nitrogen from the manure. After that, urea was added to each treatment to examine effects of residual Cu and Zn on N₂O emissions and its related factors. The results show that N₂O emissions from different pH soils had different responses to MP addition. MP significantly reduced N₂O emissions from acidic and alkaline soils, while significantly increased N₂O emissions from neutral soil (P<0.05). There were some differences in N₂O-producing substrates and microbial factors significantly related to available Cu and Zn in different soils. Further stepwise regression analysis showed that the main drivers of N₂O emissions from acidic soil were NH₄⁺-N conversion amount, available Zn content and 18S rRNA abundance. N₂O emissions from alkaline soil were decided by NO₃^--N production level only, while the main driver of N₂O emissions from neutral soil was the available Cu content. Relative to M treatment, residual available Cu in MP was significantly negatively correlated to the nirS abundance (P<0.05), while positively associated with AOB amoA abundance (n=3, r>0.95); the residual Cu had the greatest role in acidic soil compared to other soils. The residual Zn was positively correlated with nosZ(P<0.05), 18S rRNA(P<0.1), AOA amoA (P<0.1), and 16S rRNA abundance (n=3, r>0.95), and had the largest promoting effect in neutral soil. Evidently, the effective Cu and Zn residues in MP have an overall inhibitory effect on N₂O emission-related microbial genes in alkaline soil. Therefore, alkaline soil should be given priority to reduce the negative environmental and ecological effects of manure in intensive aquaculture if the manure is required to return to the field.

Key words： vegetable soil copper zinc swine manure N₂O emission

Received: 23 September 2022

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	ZHANG Ting
	JIANG Jing-jing
	FENG Lian-jie
	JIANG Jing-yan

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ZHANG Ting,JIANG Jing-jing,FENG Lian-jie等. Effects of Copper and Zinc residues in swine manure on soil N₂O emissions and related factors[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 3013-3022.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I6/3013

[1]	IPCC. Climate Change 2013:The physical science basis. Contribution of working group I to the Fifth assessment report of the Intergovernmental Panel on Climate Change[C]. UK:Cambridge University Press, 2014.
[2]	Hallin S, Philippot L, Löffler F E, et al. Genomics and ecology of novel N2O-Reducing microorganisms[J]. Trends in Microbiology, 2018,26(1):43-55.
[3]	Sasada Y, Win K T, Nonaka R, et al. Methane and N2O emissions, nitrate concentrations of drainage water, and zinc and copper uptake by rice fertilized with anaerobically digested cattle or pig slurry[J]. Biology and Fertility of Soils, 2011,47:949-956.
[4]	朱永官,王晓辉,杨小茹,等.农田土壤N2O产生的关键微生物过程及减排措施[J]. 环境科学, 2014,35(2):792-800. Zhu Y G, Wang X H, Yang X R, et al. Key microbial processes in nitrous oxide emissions of agricultural soil and mitigation strategies[J]. Environmental Science, 2014,35(2):792-800.
[5]	Hu H W, Chen D, He J Z. Microbial regulation of terrestrial nitrous oxide formation:Understanding the biological pathways for prediction of emission rates[J]. FEMS Microbiology Reviews, 2015,39(5):729-749.
[6]	Yang Y D, Nie J W, Wang S, et al. Differentiated responses of nirS-and nirK-type denitrifiers to 30years of combined inorganic and organic fertilization in a paddy soil[J]. Archives of Agronomy and Soil Science, 2021,67(1):79-92.
[7]	朱志平,董红敏,魏莎,等.中国畜禽粪便管理变化对温室气体排放的影响[J]. 农业环境科学学报, 2020,39(4):743-748. Zhu Z P, Dong H M, Wei S, et al. Impact of changes in livestock manure management on greenhouse gas emissions in China[J]. Journal of Agro-Environment Science, 2020,39(4):743-748.
[8]	Philippot L, Hallin S, Schloter M. Ecology of denitrifying prokaryotes in agricultural soil[J]. Advances in Agronomy, 2007,96(7):249-305.
[9]	王方浩,马文奇,窦争霞,等.中国畜禽粪便产生量估算及环境效应[J]. 中国环境科学, 2006,26(5):614-617. Wang F H, Ma W Q, Dou Z X, et al. The estimation of the production amount of animal manure and its environmental effect in China[J]. China Environmental Science, 2006,26(5):614-617.
[10]	庞妍,唐希望,吉普辉,等.关中平原畜禽粪便重金属农用风险估算[J]. 中国环境科学, 2015,35(12):3824-3832. Pang Y, Tang X W, Ji P H, et al. The agricultural pollution risk estimation of livestock manures on heavy metals in Guanzhong plain[J]. China Environmental Science, 2015,35(12):3824-3832.
[11]	Wang H, Dong Y H, Yang Y Y, et al. Changes in heavy metal contents in animal feeds and manures in an intensive animal production region of China[J]. Journal of Environmental Sciences, 2013,25(12):2435- 2442.
[12]	Peng H, Chen Y L, Weng L P, et al. Comparisons of heavy metal input inventory in agricultural soils in north and south China:a review[J]. Science of the Total Environment, 2019,660:776-786.
[13]	Liu W R, Zeng D, She L, et al. Comparisons of pollution characteristics, emission situations, and mass loads for heavy metals in the manures of different livestock and poultry in China[J]. Science of the Total Environment, 2020,734:139023.
[14]	Hu B F, Shao S, Ni H, et al. Current status, spatial features, health risks, and potential driving factors of soil heavy metal pollution in China at province level[J]. Environmental Pollution, 2020,266:114961.
[15]	Fermoso F G, Bartacek J, Jansen S, et al. Metal supplementation to UASB bioreactors:from cell-metal interactions to full-scale application[J]. Science of the Total Environment, 2009,407:3652-3667.
[16]	Walker C B, De La Torre J R, Klotz M G, et al. Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010,107(19):8818-8823.
[17]	Montoya M, Castellano-Hinojosa A, Vallejo A, et al. Zinc fertilizers influence greenhouse gas emissions and nitrifying and denitrifying communities in a non-irrigated arable cropland[J]. Geoderma, 2018, 325:208-217.
[18]	Yin S X, Yang L Z, Yin B, et al. Nitrification and denitrification activities of zinc-treated soils worked by the earthworm Pheretima sp[J]. Biology and Fertility of Soils, 2003,38(3):176-180.
[19]	陈中云,闵航,张夫道,等.4种重金属污染对稻田土反硝化细菌的影响[J]. 中国环境科学, 2003,23(3):305-310. Chen Z Y, Min H, Zhang F D, et al. Effect of four kinds of heavy metal contamination on denitrifying bacteria in paddy soils[J]. China Environmental Science, 2003,23(3):305-310.
[20]	李平,郎漫,李煜姗,等.施用猪粪条件下重金属对土壤氮素净转化的影响[J]. 土壤通报, 2017,48(2):467-473. Li P, Lang M, Li Y S, et al. Effects of heavy metals on net nitrogen transformation in soil with pig manure applied[J]. Chinese Journal of Soil Science, 2017,48(2):467-473.
[21]	Ma Y B, Lombi E, Oliver I W, et al. Long-term aging of copper added to soils[J]. Environmental Science & Technology, 2006,40(20):6310-6317.
[22]	Guo G C, Yuan T, Wang W W, et al. Effect of aging on bioavailability of copper on the fluvo aquic soil[J]. International Journal of Environmental Science and Technology, 2011,8(4):715-722.
[23]	Ovreås L, Forney L, Daae F L, et al. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA[J]. Applied and Environmental Microbiology, 1997,63 (9):3367-3373.
[24]	Knerr A, Tripepi R R. Changes in bacterial communities in dairy manure during nine months of composting as determined by denaturing gradient gel electrophoresis[C]. International Symposium on Growing Media and Soilless Cultivation, 2014,1034:399-407.
[25]	Tourna M, Freitag T E, Nicol G W, et al. Growth, activity and temperature responses of ammonia-oxidizing archaea and bacteria in soil microcosms[J]. Environmental Microbiology, 2008,10(5):1357-1364.
[26]	Stephen J R, Chang Y J, Macnaughton S J, et al. Effect of toxic metals on indigenous soil β-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria[J]. Applied and Environmental Microbiology, 1999,65(1):95-101.
[27]	Henry S, Baudoin E, López-Gutiérrez J C, et al. Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR[J]. Journal of Microbiological Methods, 2004,59(3):327-335.
[28]	Braker G, Fesefelda A, Witzel K P. Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples[J]. Applied and Environmental Microbiology, 1998,64(10):3769-3775.
[29]	Scala D J, Kerkhof L J. Nitrous oxide reductase (nosZ) gene-specific PCR primers for detection of denitrifiers and three nosZ genes from marine sediments[J]. FEMS Microbiology Letters, 1998,162(1):61-68.
[30]	Li Z L, Zeng Z Q, Tian D S, et al. Global patterns and controlling factors of soil nitrification rate[J]. Global Change Biology, 2020, 26(7):4147-4157.
[31]	杨云,黄耀,姜纪峰.土壤理化特性对冬季菜地N2O排放的影响[J]. 农村生态环境, 2005,21(2):7-12. Yang Y, Huang Y, Jiang J F. Influence of soil properties on N2O emission from vegetable soils[J]. Rural Eco-Environment, 2005,21(2):7-12.
[32]	Qin F, Shan X Q, Wei B. Effects of low-molecular-weight organic acids and residence time on desorption of Cu, Cd, and Pb from soils[J]. Chemosphere, 2004,57(4):253-263.
[33]	常春英,曹浩轩,陶亮,等.固化/稳定化修复后土壤重金属稳定性及再活化研究进展[J]. 土壤, 2021,53(4):682-691. Chang C Y, Cao H X, Tao L, et al. Advances on heavy metal stability and reactivation for soil after solidification/stabilization remediation[J]. Soils, 2021,53(4):682-691.
[34]	Shcherbak I, Millar N, Robertson G P. Global meta-analysis of the nonlinear response of soil nitrous oxide (N2O) emissions to fertilizer nitrogen[J]. Proceedings of the National Academy of Sciences, 2014, 111(25):9199-9204.
[35]	Glass J B, Orphan V J. Trace metal requirements for microbial enzymes involved in the production and consumption of methane and nitrous oxide[J]. Frontiers in Microbiology, 2012,3:1-20.
[36]	周国华.土壤重金属生物有效性研究进展[J]. 物探与化探, 2014, 38(6):1097-1106. Zhou G H. Recent progress in the study of heavy metal bioavailability in soil[J]. Geophysical and Geochemical Exploration, 2014,38(6):1097-1106.
[37]	滕应,黄昌勇.重金属污染土壤的微生物生态效应及其修复研究进展[J]. 土壤与环境, 2002,11(1):85-89. Teng Y, Huang C Y. Research progress on microbial ecological effects and remediation of heavy metal contaminated soil[J]. Soil and Environmental Sciences, 2002,11(1):85-89.
[38]	Tripathi B M, Stegen J C, Kim M, et al. Soil pH mediates the balance between stochastic and deterministic assembly of bacteria[J]. ISME Journal, 2018,12:1072-1083.
[39]	Lammel D R, Barth G, Ovaskainen O, et al. Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures[J]. Microbiome, 2018,106(6):2-13.
[40]	周金蓉.畜禽粪肥中典型重金属(Cu和Zn)对农田土壤N2O排放的影响及其机制[D]. 南京:南京农业大学, 2022. Zhou J R. Effects of typical heavy metals (Copper and Zinc) in livestock and poultry manure on nitrous oxide emissions from farmland soils and the mechanisms[D]. Nanjing:Nanjing Agricultural University, 2022.