澜沧江沉积物氨氧化及反硝化微生物丰度

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Abstract In order to explore the impact of cascade reservoir on nitrogen cycling microorganisms in the Lancang River , the abundance of ammonia oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and nirS type denitrifying microorganisms in the river sediments were studied. Sediment samples were collected from 19 sites from Yanjing to Ganlanba in Yunnan Province. The abundance of AOB-amoA, AOA-amoA and nirS genes were quantified through qPCR. The physical and chemical factors of sediments, pore water, and water bodies were determined at the mean time. The results showed that AOB-amoA gene abundance ranged from 0.82x10⁷ to 4.25x10⁷ copies/g, AOA-amoA gene abundance ranged from 0.83x10⁷ to 6.87x10⁷ copies/g, and the nirS gene abundance ranged from 0.72x10⁷ to 7.32x10⁷ copies/g; the ratio of AOA/AOB is between 0.35 and 2.17, and AOA is not superior to AOB in the sediment. The abundance of AOA-amoA, AOB-amoA and nirS showed no significant difference in the river, reservoir and tributaries sites, indicating that the dam construction has no significant impact on the spatial distribution of nitrogen cycling microorganism abundances. Ammonia, total phosphorus and total nitrogen concentration in pore water are the main environmental factors affecting AOA abundance. AOB abundance is mainly affected by water temperature, total phosphorus, total nitrogen and total organic carbon content in sediments. The abundance of nirS type denitrifying microorganisms is mainly affected by total nitrogen concentration in pore water, and organic carbon content in sediment.

Key words： Lancang River ammonia oxidizing archae ammonia oxidizing bacteria nirS type denitrifying microorganisms sediment

Received: 26 September 2022

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X171.5

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	WU Zi-xian
	CHEN Hao-dong
	LI Zhu
	CHEN Zhuo
	LIAO Ming-jun

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WU Zi-xian,CHEN Hao-dong,LI Zhu等. Abundance of aerobic ammonia-oxidizing and nirS type denitrifying microorganisms in sediments of Lancang River[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 3107-3117.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I6/3107

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