氨氮浓度对生物膜磷富集效果的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (674 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In this paper, the effects of ammonia concentration in synthetic sewage on the phosphorus (P) enrichment performance, P uptake and release rate of biofilm were investigated in an anaerobic/aerobic polyphosphate accumulating organisms biofilm reactor. The results showed that an increase of ammonia from 15mg-N/L to 30mg-N/L stimulated the P uptake rate of denitrifying polyphosphate accumulating organisms while weaken the PAOs, which resulted in the phosphate uptake rates (PUR) of 11.25mg-P/(L·h),and phosphate release rate (PRR) of 12.02mg-P/(L·h) for the biofilm system. The P concentration in enriched stream reached 52mg-P/L. The uptake and release of P mainly depended on DPAOs when further increase of ammonia to 40mg-N/L, whereas the PAOs metabolism was almost suppressed. As a consequence, the P concentration in enrichment stream was only 40mg-P/L with 8.65mg-P/(L·h) of PUR and 8.81mg-P/(L·h) of PRR. Stoichiometric analysis showed the P_rel/HAc_upt(the P released per organics uptake) of the biofilm system witnessed a rising and decent trend, with an increase of ammonia from 15mg-N/L to 40mg-N/L. The average values of P_rel/HAc_uptunder different ammonia concentrations were 0.06, 0.11and 0.07P-mmol/C-mmol, respectively. The adverse effect of ammonia (40mg-N/L) can be eliminat by decrease the volume ratio of sewage stream and enrichment stream (from 3:1 to 1.5:1). Specifically, the PUR and PRR of biofilm reached 16.25mg-P/(L·h) and 15.60mg-P/(L·h) respectively, resulting in a enrichment stream of 70mg-P/L. Meanwhile, the P_rel/HAc_upt rised up to 0.29P-mmol/C-mmol. The resuls of present study showed that the P enrichment performance in PAOs-biofilm was not affected by ammonia (< 40mg-N/L), also, the nitrogen removal efficiency reached 95%. Therefore, PAOs-biofilm-based technology is of great potential in simultaneous P enrichment and nitrogen removal from sewage.

Key words： ammonia concentration biofilm sequencing batch reactor phosphorus uptake and release rates P_rel/HAc_upt

Received: 06 December 2022

PACS:

X703.1

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	BI Zhen
	ZHANG Sheng
	FU Hao
	DING Ruo Ling
	HUANG Yong

Cite this article:

BI Zhen,ZHANG Sheng,FU Hao等. Effect of ammonia nitrogen concentration on phosphorus enrichment from biofilm[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3447-3453.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I7/3447

[1]	Kodera H, Hatamoto M, Abe K, et al. Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor [J]. Water Research, 2013,47(6):2025-2032.
[2]	Wong P Y, Cheng K Y, Kaksonen A H, et al. A novel post denitrification configuration for phosphorus recovery using polyphosphate accumulating organisms [J]. Water Research, 2013,47 (17):6488-6495.
[3]	Marti N, Pastor L, Bouzas A, et al. Phosphorus recovery by struvite crystallization in WWTPs: Influence of the sludge treatment line operation [J]. Water Research, 2010,44(7):2371-2379.
[4]	Melia P M, Cundy A B, Sohi S P, et al. Trends in the recovery of phosphorus in bioavailable forms from wastewater [J]. Chemosphere, 2017,186:381-395.
[5]	Tian Q, Ong S K, Xie X, et al. Enhanced phosphorus recovery and biofilm microbial community changes in an alternating anaerobic/ aerobic biofilter [J]. Chemosphere, 2016,144:1797-1806.
[6]	Zhang H, Bi Z, Pan Y, et al. Enhanced phosphorus storage in suspended biofilm by increasing dissolved oxygen [J]. Science of the Total Environment, 2020,722:137876.
[7]	杨婉静,潘杨,陈越,等.胞外聚合物在生物膜同步去除/富集磷酸盐系统中的作用 [J]. 环境科学学报, 2021,41(9):3437-3445. Yang W J, Pan Y, Chen Y, et al. The role of extracellular polymer substances in simultaneous removal/enrichment of phosphate in biofilms system [J]. Acta Scientiae Circumstantiae, 2021,41(9):3437-3445.
[8]	毕贞,黄勇,潘杨,等.一种利用城市污水有机碳源的磷去除与回收方法 [P]. 江苏省:CN111646573B,2021-11-23. Bi Z, Huang Y, Pan Y, et al. A method for phosphorus removal and recovery using organic carbon sources in urban sewage [P]. Jiangsu Province: CN111646573B,2021-11-23.
[9]	Zou J, Li Y, Zhang L, et al. Understanding the impact of influent nitrogen concentration on granule size and microbial community in a granule-based enhanced biological phosphorus removal system [J]. Bioresource Technology, 2015,177:209-216.
[10]	李冬,曹美钟,郭跃洲,等.进水氨氮浓度对生物除磷颗粒系统的影响 [J]. 环境科学, 2019,40(3):1360-1366. Li D, Cao Meizhong, Guo Yuezhou, et al. The effect of influent ammonia nitrogen concentration on biological phosphorus removal particle systems [J]. Environmental Science, 2019,40(3):1360-1366.
[11]	Xu D, Chen H, Li X, et al. Enhanced biological nutrient removal in sequencing batch reactors operated as static/oxic/anoxic (SOA) process [J]. Bioresource technology, 2013,143:204-211.
[12]	Daims H, Lebedeva E V, Pjevac P, et al. Complete nitrification by Nitrospira bacteria [J]. Nature, 2015,528(7583):504-509.
[13]	Camejo P Y, Owen B R, Martirano J, et al. Candidatus Accumulibacter phosphatis clades enriched under cyclic anaerobic and microaerobic conditions simultaneously use different electron acceptors [J]. Water Research, 2016,102:125-137.
[14]	Rubio-Rincón F J, Lopez-Vazquez C M, Welles L, et al. Cooperation between Candidatus Competibacter and Candidatus Accumulibacter clade I, in denitrification and phosphate removal processes [J]. Water Research, 2017,120:156-164.
[15]	Rubio-Rincón F J, Weissbrodt D G, Lopez-Vazquez C M, et al. “Candidatus Accumulibacter delftensis”: A clade IC novel polyphosphate-accumulating organism without denitrifying activity on nitrate [J]. Water Research, 2019,161:136-151.
[16]	Tian Q, Zhuang L, Ong S K, et al. Phosphorus (P) recovery coupled with increasing influent ammonium facilitated intracellular carbon source storage and simultaneous aerobic phosphorus & nitrogen removal [J]. Water Research, 2017,119:267-275.
[17]	Yang W, Shan J, Pan Y, et al. A new strategy for obtaining highly concentrated phosphorus recovery solution in biofilm phosphorus recovery process [J]. Journal of Environmental Sciences, 2022,112: 366-375.
[18]	Ni M, Pan Y, Chen Y, et al. Effects of seasonal temperature variations on phosphorus removal, recovery, and key metabolic pathways in the suspended biofilm [J]. Biochemical Engineering Journal, 2021,176: 108187.
[19]	丁鑫.利用城市污水有机碳的生物膜磷富集工艺研究 [D]. 苏州:苏州科技大学, 2021. Ding X. Research on biofilm phosphorus recovery process using organic carbon from municipal wastewater [D]. Suzhou: Suzhou University of Science and Technology, 2021.
[20]	曲久辉,赵进才,任南琪,等.城市污水再生与循环利用的关键基础科学问题 [J]. 中国基础科学, 2017,19(1):6-12. Qu Jiuhui, Zhao Jincai, Ren Nanqi, et al. Critical fundamental scientific problems in reclamation and reuse of municipal wastewater [J]. China Basic Science, 2017,19(1):6-12.
[21]	李冬,崔少明,梁瑜海,等.溶解氧对序批式全程自养脱氮工艺运行的影响 [J]. 中国环境科学, 2014,34(5):1131-1138. Li D, Cui Shaoming, Liang Yuhai, et al. The effect of dissolved oxygen on the operation of sequencing batch full autotrophic nitrogen removal process [J]. Chinese Environmental Science, 2014,34(5): 1131-1138.
[22]	Oehmen A, Saunders A M, Vives M T, et al. Competition between polyphosphate and glycogen accumulating organisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources [J]. Journal of Biotechnology, 2006,123(1):22-32.
[23]	Kerrn-Jespersen J P, Henze M. Biological phosphorus uptake under anoxic and aerobic conditions [J]. Water Research, 1993,27(4):617-624.
[24]	Coma M, Puig S, Balaguer M D, et al. The role of nitrate and nitrite in a granular sludge process treating low-strength wastewater [J]. Chemical Engineering Journal, 2010,164(1):208-213.
[25]	吕小梅,李继,李朝林,等.不同电子受体除磷污泥相似性与菌群结构研究 [J]. 中国环境科学, 2014,34(4):935-941. Lv Xiaomei, Li Ji, Li Chaolin, et al. Microbial similarity and community structure of phosphorus removal sludge with different electron acceptors [J]. China Environmental Science, 2014,34(4):935-941.
[26]	赵伟华,李健伟,王梅香,等.前置A2NSBR系统硝化和反硝化除磷的特性 [J]. 中国环境科学, 2019,39(11):4660-4665. Zhao Weihua, Li Jianwei, Wang Meixiang, et al. Nitrification and denitrifying phosphorus removal performance in the pre-A2NSBR system [J]. China Environmental Science, 2019,39(11):4660-4665.
[27]	Li H, Zhong Y, Huang H, et al. Simultaneous nitrogen and phosphorus removal by interactions between phosphate accumulating organisms (PAOs) and denitrifying phosphate accumulating organisms (DPAOs) in a sequencing batch reactor [J]. Science of the Total Environment, 2020,744:140852.
[28]	Wang X, Wang S, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR) [J]. Water Research, 2016,95:319-329.
[29]	潘婷,张淼,范亚骏,等.基于碳源优化的反硝化除磷及微生物特性 [J]. 中国环境科学, 2020,40(7):2901-2908. Pan Ting, Zhang Miao, Fan Yajun, et al. Denitrifying phosphorus removal and microbial characteristics based on the optimization of carbon sources [J]. China Environmental Science, 2020,40(7):2901-2908.
[30]	Kodera H, Hatamoto M, Abe K, et al. Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor [J]. Water Research, 2013,47(6):2025-2032.
[31]	Long X Y, Tang R, Wang T, et al. Characteristics of enhanced biological phosphorus removal (EBPR) process under the combined actions of intracellular and extracellular polyphosphate [J]. Chemosphere, 2021,279:130912.
[32]	Wong P Y, Cheng K Y, Krishna K C B, et al. Improvement of carbon usage for phosphorus recovery in EBPR-r and the shift in microbial community [J]. Journal of Environmental Management, 2018,218: 569-578.
[33]	单捷,潘杨,章豪,等.基于生物膜法磷回收工艺厌氧释磷研究 [J]. 环境科学学报, 2020,40(8):2749-2757. Shan Jie, Pan Yang, Zhang Hao, et al. The anaerobic phosphorus release of biofilm-based phosphorus recovery process [J]. Journal of Environmental Science, 2020,40(8):2749-2757.
[34]	张建华,王淑莹,张淼,等.不同反应时间内碳源转化对反硝化除磷的影响 [J]. 中国环境科学, 2017,37(3):989-997. Zhang Jianhua, Wang Shuying, Zhang Miao, et al. Effect of conversion of internal carbon source on denitrifying phosphorus removal under different reaction time [J]. China Environmental Science, 2017,37(3):989-997.
[35]	王琪,李冬,李鹏垚,等.厌/缺氧时间对好氧颗粒污泥同步硝化内源反硝化和除磷的影响 [J]. 中国环境科学, 2022,42(9):4199-4206. Wang Qi, Li Dong, Li Pengyao, et al. Effects of anaerobic/anoxic time on simultaneous nitrification-endogenous denitrification and phosphorous removal from aerobic granular sludge [J]. China Environmental Science, 2022,42(9):4199-4206.
[36]	许德超,陈洪波,李小明,等.进水氨氮浓度对静置/好氧/缺氧SBR脱氮除磷性能的影响 [J]. 中国环境科学, 2013,33(11):1984-1992. Xu Dechao, Chen Hongbo, Li Xiaoming, et al. The effect of influent ammonia nitrogen concentration on the nitrogen and phosphorus removal performance of static/aerobic/anoxic SBR [J]. Chinese Environmental Science, 2013,33(11):1984-1992.