底物对厌氧消化系统EPS产生及起泡的影响

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Abstract To clarify the influence of substrates composition on the production characteristics and foaming performance of extracellular polymer (EPS) in anaerobic digestion system, three types of food waste, namely high-carbohydrate, high-protein and high-fat, were tested in batch experiments. The EPS production characteristics (concentration, composition, structure, functional groups, etc.), and parameters characterizing the foaming properties (viscosity, surface tension and relative hydrophobicity) and potential (foaming tendency, foam stability, foam diameter and liquid film thickness) of various types of EPS and sludge, were examined in different test groups. The correlation between EPS characteristics and sludge characteristics was also analyzed. The test groups with different substrates produced EPS with different properties. Specifically, the more balanced the substrate composition, the more it tended to be used for gas production, while the high carbohydrate and control groups had significantly higher total EPS concentrations than the rest experimental groups due to the excess carbohydrate content. Carbohydrates, proteins and oils influenced EPS morphology and composition by altering intermediate metabolites, affecting enzyme production and proliferation of microorganisms, respectively. In terms of EPS structure, more humic-like substances were produced under high carbohydrate conditions in the high-carbohydrate and control groups. In terms of functional groups, the alkyl group was reduced in the high-carbohydrate group, the high-protein group produced more hydrophilic nitrogenous functional groups, and the carboxylic acid content increased in the high-fat group. In addition, the relative hydrophobicity of EPS was reduced to varying degrees in all three experimental groups compared to the control group. The correlation analysis revealed that the sludge foaming capacity was significantly more correlated with EPS-related parameters than with substrate categories. Sludge foaming tendency (FP) was strongly correlated with loosely bound EPS (P < 0.01, r=0.979) and soluble EPS (P < 0.05, r=0.678). EPS viscosity also had a positive effect on both FP and foam stability (FS) (P < 0.05), and increased EPS surface tension favored the FS (P < 0.05). In terms of the role of substrates on foaming, carbohydrates may be responsible for the production of larger diameter, more stable foams from the sludge by increasing EPS viscosity and hydrophilicity. Proteins use enzymes to reduce the surface tension of EPS, resulting in smaller diameter and less stable foams, and thus alleviated the foaming phenomenon. Fat reduced the loosely bound EPS (P < 0.05, r =-0.649) and its intermediate metabolite LCFA altered the viscosity of tightly bound EPS (P < 0.01, r=0.788), both contributing to increase the FP.

Key words： anaerobic digestion food waste foaming EPS

Received: 21 December 2022

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X705

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	ZHOU Ying-yue
	ZHANG Zhi
	LI Lei
	YANG Ping-jin
	WANG Xiao-ming
	PENG Xu-ya

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ZHOU Ying-yue,ZHANG Zhi,LI Lei等. Influence of substrate composition on the production characteristics of extracellular polymers and foaming performance in anaerobic digestion systems[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 4046-4056.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I8/4046

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