赤泥基生物炭载体对厌氧氨氧化污泥富集的影响研究

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2192 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To achieve rapid enrichment of anaerobic ammonia oxidation (Anammox) sludge, the red mud-based biochar carrier was prepared using red mud as the iron source and Erianthus arundinaceus straw as the biochar precursor, and then mixed it with the natural pond sludge treated for aquaculture digestate and dosed into an up-flow anaerobic sludge bed (UASB) reactor. The changes in the nitrogen removal performance, sludge performance and microbial community structure of reactors during the domestication of Anammox sludge were investigated, and the effect mechanism of the red mud-based biochar carriers on Anammox sludge enrichment was evaluated. We found that the Anammox sludge was successfully enriched in R2reactor containing red mud-based biochar carrier after 59days incubation, and the removal rates of NH₄⁺-N and NO₂^--N were 1.47kg/(m³·d) and 1.91kg/(m³·d), respectively. Whereas the Anammox sludge enrichment was happened in R1reactor without carrier at day 92. Compared with R1 reactor, R2 reactor showed significantly effective in the process of Anammox sludge domestication, including the extracellular polymeric substances (EPS) secretion, sludge particle size and granulation. At the end of domestication, high population of anaerobic ammonia oxidizing bacteria (AnAOB) attached to the pores of red mud-based biochar carrier and accumulated iron surrounding AnAOB were observed in R2reactor, while the AnAOB from R1reactor were relatively small and dispersed. The specific anaerobic ammonia oxidation activity (SAA) of R2 was 2.3 times higher than that of R1, and the Anammox gene copy number from R2 was increased by 3.8times comparison from R1 at the end of domestication. These results suggest that the red mud-based biochar carrier could markedly accelerate the enrichment of Anammox sludge. This carrier not only provides a "house" for AnAOB to resist the unfavorable environment, also plays a role in the metabolism and agglomeration growth of AnAOB which depends on the active components of red mud, iron. Importantly, the red mud-based biochar carrier was made of solid waste, which is economically effective in practical applications.

Key words： anaerobic ammonia oxidation sludge enrichment red mud biochar iron

Received: 06 February 2023

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	QIN Yong-li
	WEI Qiao-yan
	JIANG Yong-rong
	CHEN Rui-hong
	LIAO Ji-qing
	YAN Jia-qi
	WU You

Cite this article:

QIN Yong-li,WEI Qiao-yan,JIANG Yong-rong等. Effect of red mud-based biochar carrier on the enrichment of anaerobic ammonia oxidation sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4728-4739.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4728

[1]	Ge S, Wang S, Yang X, et al. Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment:A review[J]. Chemosphere, 2015,140:85-98.
[2]	张涵,张肖静,马冰冰,等.以城市废弃污泥为种泥启动厌氧氨氧化工艺的可行性[J/OL]. 化工进展, 2022,https://doi.org/10.16085/j.issn.1000-6613.2022-0742. Zhang H, Zhang X J, Ma B B, et al. Feasibility of starting anammox process with municipal waste sludge as seed sludge[J]. Chemical Industry and Engineering Progress, 2022. https://doi.org/10.16085/j.issn.1000-6613.2022-0742.
[3]	Kartal B, Kuenen J G, Loosdrecht M C M. Sewage treatment with anammox[J]. Science, 2010,328(5979):702-703.
[4]	Kumwimba M N, Lotti T, Şenel E, et al. Anammox-based processes:how far have we come and what work remains? A review by bibliometric analysis[J]. Chemosphere, 2020,238:124627.
[5]	Liang Z, Liu J. Landfill leachate treatment with a novel process:Anaerobic ammonium oxidation (Anammox) combined with soil infiltration system[J]. Journal of Hazardous Materials, 2008,151:202-212.
[6]	Joss A, Salzgeber D, Eugster J, et al. Full-scale nitrogen removal from digester liquid with partial nitritation and anammox in one SBR[J]. Environmental Science & Technology, 2009,43(14):5301-5306.
[7]	Liu W, Wang Q, Shen Y, et al. Enhancing the in-situ enrichment of anammox bacteria in aerobic granules to achieve high-rate CANON at low temperatures[J]. Chemosphere, 2021,278:130395.
[8]	Yan Y, Wang Y, Wang W, et al. Comparison of short-term dosing ferrous ion and nanoscale zero-valent iron for rapid recovery of anammox activity from dissolved oxygen inhibition[J]. Water Research, 2019,153:284-294.
[9]	Li Z, Peng Y, Gao H. A novel strategy for accelerating the recovery of a Fe(II)-inhibited anammox reactor by intermittent addition of betaine:Performance, kinetics and statistical analysis[J]. Chemosphere, 2020,251:126362.
[10]	Zang L, Narita Y, Gao L, et al. Maximum specific growth rate of anammox bacteria revisited[J]. Water Research, 2017,116:296-303.
[11]	Strous M, Kuenen J G, Jetten M S. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology, 1999,65(7):3248-3250.
[12]	Huang Q, Du W L, Miao L L, et al. Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance[J]. AMB Express, 2018,8(1):156.
[13]	黄方玉,邓良伟,杨红男,等.温度对自养型同步脱氮工艺处理猪场废水厌氧消化液性能及微生物群落的影响[J]. 环境科学, 2019,40(5):2357-2367. Huang F Y, Deng L W, Yang H N, et al. Effect of different temperatures on the performance of autotrophic nitrogen removal and microbial community from swine wastewater[J]. Environmental Science, 2019,40(5):2357-2367.
[14]	Yu J J, Chen H, Zhang J, et al. Enhancement of Anammox activity by low-intensity ultrasound irradiation at ambient temperature[J]. Bioresource Technology, 2013,2:693-696.
[15]	张亚超,张晶,侯爱月,等.胞外聚合物和信号分子对厌氧氨氧化污泥活性的影响[J]. 中国环境科学, 2019,39(10):4133-4140. Zhang Y C, Zhang J, Hou A Y, et al. Effect of extracellular polymers and signal molecules on the activity of ANAMMOX sludge[J]. China Environmental Science, 2019,39(10):4133-4140.
[16]	Liu Y, Ni B J. Appropriate Fe (II) Addition significantly enhances anaerobic ammonium oxidation (Anammox) activity through improving the bacterial growth rate[J]. Scientific Reports, 2015,5:8204.
[17]	Liu W R, Li T H, Wang J F, et al. A new concept of waste iron recycling for the enhancement of the anammox process[J]. Chemosphere, 2022,307:136151.
[18]	Wang J X, Liang J D, Ning D Y, et al. A review of biomass immobilization in anammox and partial nitrification/anammox systems:Advances, issues, and future perspectives[J]. Science of the Total Environment, 2022,821:152792.
[19]	Kumwimba M N, Lotti T, Şenel E, et al. Anammox-based processes:how far have we come and what work remains?A review by bibliometric analysis[J]. Chemosphere, 2020,238:124627.
[20]	Wang J, Liang J, Sun L, et al. Granule-based immobilization and activity enhancement of anammox biomass via PVA/CS and PVA/CS/Fe gel beads[J]. Bioresource Technology, 2020,309:123448.
[21]	Gao D W, Li Y Q, Liang H. Biofilm carriers for anaerobic ammonium oxidation:Mechanisms, applications, and roles in mainstream systems[J]. Bioresource Technology, 2022,353:127115.
[22]	Xu J J, Li C, Shen Y W, et al. Anaerobic ammonium oxidation (anammox) promoted by pyrogenic biochar:Deciphering the interaction with extracellular polymeric substances (EPS)[J]. Science of the Total Environment, 2022,802:149884.
[23]	梁文俊,杨岚,张艳,等.改性赤泥吸附剂吸附低浓度CO2研究[J/OL]. 中国环境科学, 2023.https://doi.org/10.19674/j.cnki.issn1000-6923.20230103.003. Liang W J, Yang L, Zhang Y, et al. Adsorption of low concentration CO2 with modified red mud[J/OL]. China Environmental Science, 2023.https://doi.org/10.19674/j.cnki.issn1000-6923.20230103.003.
[24]	Qin Y L, Wei Q Y, Zhang Y Y, et al. Nitrogen removal from ammonium-and sulfate-rich wastewater in an upflow anaerobic sludge bed reactor:performance and microbial community structure[J]. Ecotoxicology, 2021,30:1719-1730.
[25]	国家环境保护总局.水和废水监测分析方法(第四版)[M]. 北京:中国环境科学出版社, 2002. State Environmental Protection Administration. Monitoring and analysis methods of water and wastewater (Fourth Edition)[M]. Beijing:China Environmental Press, 2002.
[26]	蒋永荣,罗娜,黄秀娟,等.固定化季铵盐对硫酸盐有机废水厌氧处理颗粒污泥特性的影响[J]. 中国环境科学, 2019,39(8):3347-3357. Jiang Y R, Luo N, Huang X J, et al. Effect of immobilized quaternary ammonium salt on properties of anaerobic granular sludge for treating sulfate organic wastewater[J]. China Environmental Science, 2019, 39(8):3347-3357.
[27]	Zhang Z Z, Cheng Y F, Zhou Y H, et al. Roles of EDTA washing and Ca2+ regulation on the restoration of anammox granules inhibited by copper(II)[J]. Journal of Hazardous Materials, 2016,301(15):92-99.
[28]	Schmid M C, Maas B, Dapena A, et al. Biomarkers for in situ detection of anaerobic ammonium-oxidizing (Anammox) bacteria[J]. Applied and Environmental Microbiology, 2005,71:1677-1684.
[29]	姚芳,刘波,王德朋,等.不同接种污泥的厌氧氨氧化反应器启动特性及菌群结构演替规律分析[J]. 环境科学学报, 2017,37(7):2543-2551. Yao F, Liu B, Wang D P, et al. Start-up of ANAMMOX enrichment with different inoculated sludge and analysis of microbial community structure shift[J]. Acta Scientiae Circumstantiae, 2017,37(7):2543-2551.
[30]	唐崇俭,郑平,陈婷婷.厌氧氨氧化工艺的菌种、启动与效能[J]. 化工学报, 2010,61(10):2510-2516. Tang C J, Zheng P, Chen T T. Inoculum, start-up and performance of Anammox process[J]. CIESC Journal, 2010,61(10):2510-2516.
[31]	Chen L, Liu F, Jia F, et al. Anaerobic ammonium oxidation in sediments of surface flow constructed wetlands treating swine wastewater[J]. Applied Microbiology and Biotechnology, 2017,101(3):1301-1311.
[32]	王泓,吴莎,刘珂,等.氮负荷提升方式强化ANAMMOX反应器的性能研究[J]. 中国给水排水, 2021,37(9):90-96. Wang H, Wu S, Liu K, et al. Performance of ANAMMOX reactor enhanced by nitrogen load lifting method[J]. China Water & Wastewater, 2021,37(9):90-96.
[33]	Pan J, Huo T, Yang H, et al. Metabolic patterns reveal enhanced anammox activity at low nitrogen conditions in the integrated I-ABR[J]. Water Environment Research, 2021,93(8):1455-1465.
[34]	Wang H, Peng L, Mao N, et al. Effects of Fe3+ on microbial communities shifts, functional genes expression and nitrogen transformation during the start-up of Anammox process[J]. Bioresource Technology, 2021,320:124326.
[35]	秦永丽,蒋永荣,刘成良,等.Anammox启动过程中脱氮性能及污泥特性研究[J]. 环境科学与技术, 2016,39(2):122-127. Qin Y L, Jiang Y R, Liu C L, et al. Study of nitrogen removal performance and sludge characteristics during start-up of Anammox[J]. Environmental Science & Technology, 2016,39(2):122-127.
[36]	Xu L Z J, Wu J, Xia W J, et al. Adaption and restoration of anammox biomass to Cd(II) stress:Performance, extracellular polymeric substance and microbial community[J]. Bioresource Technology, 2019, 290:121766.
[37]	Tang S, Xu Z, Liu Y, et al. Performance, kinetics characteristics and enhancement mechanisms in anammox process under Fe(II) enhanced conditions[J]. Biodegradation, 2020,31:223-234.
[38]	李晓光,赵颖,卓锦德,等.赤泥基Na型分子筛的制备及其去除氨氮性能研究[J]. 硅酸盐通报, 2018,37(11):3700-3706. Li X G, Zhao Y, Zhuo J D, et al. Study on synthesized zeolite na with red mud and treatment of the ammonium in wastewater[J]. Bulletin of the Chinese Ceramic Society, 2018,37(11):3700-3706.
[39]	李帅,董发勤,孙仕勇,等.纳米二氧化硅自由基对人体常驻大肠杆菌的界膜氧化作用[J]. 环境与健康杂志, 2015,32(5):392-396. Li S, Dong F Q, Sun S Y, et al. Free-radical oxidation stressing on common microbial-Escherichia coli interface induced by nano-SiO2[J]. J Environ Health, 2015,32(5):392-396.
[40]	Liu Z, Zhou L, Liu F, et al. Impact of Al-based coagulants on the formation of aerobic granules:Comparison between poly aluminum chloride (PAC) and aluminum sulfate (AS)[J]. Science of the Total Environment, 2019,685:74-84.
[41]	Kim D G, Lee K Y, Lee S J, et al. Structural and functional studies of SAV1707from Staphylococcus aureus elucidate its distinct metal-dependent activity and a crucial residue for catalysis[J]. Acta Crystallographica Section D, 2021,77(5):587-598.
[42]	Mak C Y, Lin J G, Chen W H, et al. The short-and long-term inhibitory effects of Fe (II) on anaerobic ammonium oxidizing (anammox) process[J]. Water Science and Technology, 2019,79(10):1860-1867.
[43]	Xu J, Li C, Zhu N, et al. Particle size-dependent behavior of redoxactive biochar to promote anaerobic ammonium oxidation (anammox)[J]. Chemical Engineering Journal, 2021,410:127925.
[44]	Sindhu L, Niu K, Liu X, et al. Effect of Fe2+ addition on anammox consortia, nitrogen removal performance and functional genes analysis during start-up of anammox process[J]. Journal of Water Process Engineering, 2021,43:102251.
[45]	Adams M, Xie J, Chang Y, et al. Start-up of anammox systems with different biochar amendment:Process characteristics and microbial community[J]. Science of The Total Environment, 2021,790:148242.
[46]	Wang W G, Liu Q H, Xue H, et al. The feasibility and mechanism of redox-active biochar for promoting anammox performance[J]. Science of the Total Environment, 2022,814:152813.
[47]	Gao F, Zhang H, Yang F, et al. The effects of zero-valent iron (ZVI) and ferroferric oxide (Fe3O4) on anammox activity and granulation in anaerobic continuously stirred tank reactors (CSTR)[J]. Process Biochemistry, 2014,49(11):1970-1978.