芘对土壤微生物氮转化功能菌群的影响特征

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Abstract An aerobic degradation microenvironment was constructed to analyze the effects of pyrene (12.09mg/kg) at ambient concentration on soil enzyme activities, the whole process of nitrogen transformation and related functional microorganisms. The results showed that pyrene only significantly increased urease activity on the first day of degradation, but promoted the dehydrogenase activity at both the early and late phases of degradation. The analysis of the bacterial community structure revealed that the variation of the relative abundance of ammonia-oxidizing bacteria (Nitrososphaeraceae) promoted pyrene-mediated aerobic ammonia oxidation and nitrification in the early stages of treatment and inhibited that in the late stages. In contrast, the effects of nitrogen-fixing bacteria (Bradyrhizobium, Mesorhizobium and Ensifer), urea-degrading bacteria (Roseomonas), and nitrate-reducing bacteria (Opitutus) were opposite. The quantitative analysis of functional genes showed that, despite pyrene's repressive effect on the nitrogen-fixing gene nifH at the start of the culture, the abundance of nifH showed an increasing trend, which was not consistent to the anticipated changes in microbial community structure and associated functions. Compared with changes in key enzyme activities and genes encoding the processes of ammonia oxidation and denitrification, pyrene did not significantly boost ammonia oxidation in the early stages of incubation, t severely hampered ammonia oxidation and denitrification after 15days, significantly inhibited the ammonia oxidation process. In this study, how pyrene influenced the microbial nitrogen transformation process in soil was reported, fundamental data on understanding the environmental hazard of pyrene were provided.

Key words： pyrene soil enzyme activity nitrogen cycling bacterial community nitrogen cycling processes

Received: 01 March 2023

PACS:	X172
	X171.5

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	Articles by authors
	HU Qin
	ZHANG Li-lan
	YI Mei-ling
	YANG Rui

Cite this article:

HU Qin,ZHANG Li-lan,YI Mei-ling等. Characterization of pyrene's impact on the soil functional microorganisms for nitrogen transformation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5574-5582.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I10/5574

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