生物炭对筛选菌株去除水中硝酸盐和N<sub>2</sub>O排放的影响

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Abstract To investigate the effect of modified biochar on microbial removal of nitrate with low concentration and N₂O emission from water, FeCl₃-, H₂O₂- and NaOH-modified biochars (BC-Fe, BC-H₂O₂, and BC-NaOH) were prepared by utilizing the pristine rice husk-derived biochar (BC) and characterized further. Meanwhile, a strain of denitrifying bacteria, Raoultella ornithinolytica strain KK1, was screened from activated sludge. Then BC, BC-Fe, BC-H₂O₂, and BC-NaOH were incorporated into the incubation system containing the strain, respectively and a control treatment without any biochar addition was also setup. The indoor incubation experiment on the microbial removal of nitrate (about 10mg/L, in terms of N) from simulated wastewater was carried out and the dynamic changes of N₂O emission rate and NO₃^--N, NO₂^--N, NH₄⁺-N, TN, and dissolved organic carbon (DOC) concentrations during incubation were analyzed. Results showed that the average NO₃^--N degradation rate of the screened strain was 0.10mg/(L·h) within 120h. Compared with BC, the carboxyl and lactone contents of BC-Fe and BC-H₂O₂ significantly increased and their oxidizing ability was enhanced, while the phenolic hydroxyl content of BC-NaOH significantly increased and its reducing ability was enhanced. Meanwhile, the total acidic oxygen-containing functional group contents of BC-Fe and BC-H₂O₂ significantly increased by 57.17% and 22.86%, respectively compared with BC, while the total basic oxygen-containing functional group content of BC-NaOH significantly increased by 91.67%. Compared with BC, BC-Fe and BC-H₂O₂ promoted the reduction of NO₃^--N by denitrifier, and the cumulative N₂O emissions were 3.11 and 2.17 times higher than that of the treatment with BC addition, respectively; while BC-NaOH inhibited the reduction of NO₃^--N by denitrifier, and the cumulative N₂O emission was only 49.63% that of the treatment with BC addition. The impact of biochar on microbial denitrification and N₂O emission is attributed to the differences in pH value, DOC content, and redox property of biochar caused by modification.

Key words： modified biochar nitrate with low concentration advanced denitrogenation N₂O emission

Received: 19 March 2023

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	WANG Chao-xu
	LI Yuan-kun
	LIU Yong-chao

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WANG Chao-xu,LI Yuan-kun,LIU Yong-chao. Effect of biochar on the removal of nitrate and N₂O emission from water by a screened denitrifying bacterium[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(11): 5728-5736.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I11/5728

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