InP/ZnS QDs对稀有鮈鲫子代软骨发育的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2361 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Quantum Dots (QDs) are a class of nanomaterials. With the wide application of QDs, its toxic affects on organisms are also concerned. In this experiment, InP/ZnS quantum dots were used, and female rare minnows (Gobiocypris rarus) was used as the experimental animal. Three experimental groups (200, 400, 800nmol/L) were designed. Eggs were taken and fertilized at 4 and 7 days of quantum dots exposure. Using embryo fertilization rate, survival rate, body length and full length of larvae as indicators, the larvae were observed by Albion blue dye and Hematoxylin Eosin (HE) staining. The transcript expression levels of bone developmental related genes (bmp2b, sox9, runx2b) were detected to study the effect of quantum dots on the offspring cartilage development of rare minnows. The results showed that the body length and full length of larvae in high concentration group decreased by 6.2% and 5.9%. The PQ-Meckel’s angle increased by 24.8%, mandibular length decreased by 14.6% and 15.2%, and mandibular width decreased by 10.0% and 10.7%. Craniofacial chondrocytes were swelled and reduced in number. It was found that QDs had different affects on the transcript expression levels of related genes at different development time. In conclusion, InP/ZnS quantum dots can affect the skeletal development of offspring of rare minnows.

Key words： InP/ZnS quantum dots Gobiocypris rarus toxicity skeletal development

Received: 14 April 2023

PACS:

X503.2

About author:: X503.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WU Ying-yi
	CHEN Hang
	XIE Wei-wei
	JIN Li

Cite this article:

WU Ying-yi,CHEN Hang,XIE Wei-wei等. Effects of InP/ZnS QDs on cartilage development in rare minnow (Gobiocypris rarus) offspring[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(12): 6732-6739.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I12/6732

[1] Han C Y, Kim H S, Yang H. Quantum dots and applications[J]. Materials (Basel), 2020,13(4):897.
[2] Jha S, Mathur P, Ramteke S, et al. Pharmaceutical potential of quantum dots[J]. Artificial Cells, Nanomedicine, and Biotechnology, 2018,46(S1):57-65.
[3] Aedel-Salam M, Omran B, Whitehead K, et al. Superior properties and biomedical applications of microorganism-derived fluorescent quantum dots[J]. Molecules (Basel, Switzerland), 2020,25(19):4486.
[4] Liang Y, Zhang T, Tang M. Toxicity of quantum dots on target organs and immune system[J]. Journal of Applied Toxicology: JAT, 2022, 42(1):17-40.
[5] Li L, Lin X, Chen T, et al. Systematic evaluation of CdSe/ZnS quantum dots toxicity on the reproduction and offspring health in male BALB/c mice[J]. Ecotoxicology and Environmental Safety, 2021,211:111946.
[6] ChenN B, Li D, Wang F. InP quantum dots: synthesis and lighting applications[J]. Small (Weinheim an der Bergstrasse, Germany), 2020, 16(32):e2002454.
[7] Lin G, Ouyang Q, Hu R, et al. In vivo toxicity assessment of non-cadmium quantum dots in BALB/c mice[J]. Nanomedicine, 2015,11(2):341-350.
[8] Liu J, Hu R, Liu J, et al. Cytotoxicity assessment of functionalized CdSe, CdTe and InP Quantum Dots in two human cancer cell models[J]. Materials Science & Engineering C, Materials for Biological Applications, 2015,57:222-231.
[9] Chen Y, Yang Y, Ou F, et al. InP/ZnS QDs exposure induces developmental toxicity in rare minnow (Gobiocypris rarus) embryos[J]. Environ Toxicol Pharmacol, 2018,60:28-36.
[10] Chen S, Chen Y, Chen Y, et al. InP/ZnS quantum dots cause inflammatory response in macrophages through endoplasmic reticulum stress and oxidative stress[J]. Int J Nanomedicine, 2019,14:9577-9586.
[11] Chen T, Li L, Xu G, et al. Cytotoxicity of InP/ZnS quantum dots with different surface functional groups toward two lung-derived cell lines[J]. Front Pharmacol, 2018,9:763.
[12] Halloran D, Durbano H W, Nohe A. Bone Morphogenetic Protein-2 in development and bone homeostasis[J]. Journal of Developmental Biology, 2020,8(3):19.
[13] Yue B, Lu B, Dai K R, et al. Bmp2 gene therapy on the repair of bone defects of aged rats[J]. Calcified Tissue International, 2005,77(6): 395-403.
[14] Chen D, Kim D J, Shen J, et al. Runx2 plays a central role in osteoarthritis development[J]. Journal of Orthopaedic Translation, 2020,23:132-139.
[15] Ding M, Lu Y, Abbassi S, et al. Targeting runx2 expression in hypertrophic chondrocytes impairs endochondral ossification during early skeletal development[J]. Journal of Cellular Physiology, 2012,227(10):3446-3456.
[16] Walker M B, Kimmel C B. A two-color acid-free cartilage and bone stain for zebrafish larvae[J]. Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission, 2007,82(1):23-28.
[17] Fan X, Wu L, Hou T, et al. Maternal bisphenol A exposure impaired endochondral ossification in craniofacial cartilage of rare minnow (Gobiocypris rarus) offspring[J]. Ecotoxicology and Environmental Safety, 2018,163:514-520.
[18] Chen J, Chen H, Wu Y, et al. Parental exposure to CdSe/ZnS QDs affects cartilage development in rare minnow (Gobiocypris rarus) offspring[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2022,256:109304.
[19] Chen H, Wu Y, XIE W, et al. InP/ZnS quantum dots cause liver damage in rare minnow (Gobiocypris rarus) larvae[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2023,266:109546.
[20] Liu L, Xiao Y Y, Ji Y H, et al. CuInS(2)/ZnS QDs exposure induces developmental toxicity, oxidative stress and DNA damage in rare minnow (Gobiocypris rarus) embryos and larvae[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2017,198:19-27.
[21] Mork L, Crump G. Zebrafish craniofacial development: A window into early patterning[J]. Current Topics in Developmental Biology, 2015, 115:235-269.
[22] Truong B T, Artinger K B. The power of zebrafish models for understanding the co-occurrence of craniofacial and limb disorders[J]. Genesis (New York, NY: 2000), 2021,59(1/2):e23407.
[23] Liu S, Narumi R, Ikeda N,et al. Chemical-induced craniofacial anomalies caused by disruption of neural crest cell development in a zebrafish model[J]. Developmental Dynamics: an Official Publication of the American Association of Anatomists, 2020,249(7):794-815.
[24] Xue Y, Zheng X, Huang L, et al. Organizer-derived bmp2 is required for the formation of a correct bmp activity gradient during embryonic development[J]. Nature Communications, 2014,5:3766.
[25] Sun J, Li J, Li C, et al. Role of Bone Morphogenetic Protein-2 in Osteogenic differentiation of mesenchymal stem cells[J]. Molecular Medicine Reports, 2015,12(3):4230-4237.
[26] Zhou N, Li Q, Lin X, et al. Bmp2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells[J]. Cell and Tissue Research, 2016,366(1):101-111.
[27] Ma X, Yang J, Liu T, et al. Gukang capsule promotes fracture healing by activating BMP/SMAD and Wnt/β-Catenin signaling pathways[J]. Evidence-based Complementary and Alternative Medicine, 2020, 2020:7184502.
[28] Lefebvre V, Dvir-Ginzberg M. Sox9 and the many facets of its regulation in the chondrocyte lineage[J]. Connective Tissue Research, 2017,58(1):2-14.
[29] Hollander J M, Zeng L. The emerging role of glucose metabolism in cartilage development[J]. Current Osteoporosis Reports, 2019,17(2): 59-69.
[30] Akiyama H, Chaboissier M C, Martin J F, et al. The transcription factor sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of sox5 and sox6[J]. Genes & Development, 2002,16(21):2813-2828.
[31] Rashid H, Chen H, Javed A. Runx2 is required for hypertrophic chondrocyte mediated degradation of cartilage matrix during endochondral ossification[J]. Matrix Biology Plus, 2021,12:100088.
[32] Ueta C, Iwamoto M, Kanatani N, et al. Skeletal malformations caused by overexpression of Cbfa1 or its dominant negative form in chondrocytes[J]. J Cell Biol, 2001,153(1):87-100.
[33] Nishimura R, Hata K, Matsubra T, et al. Regulation of bone and cartilage development by network between BMP signalling and transcription factors[J]. J Biochem, 2012,151(3):247-254.
[34] 曾宣,施志仪,陈晓武,等.褐牙鲆变态期间骨骼发育及其相关基因sox9,bmp4和bmp2的表达分析[J]. 海洋渔业, 2009,31(4):337-346. Zeng X, Shi Z Y, Chen X W, et al. Skeleton development of Japanese flounder (Paralichthys olivaceus) and expression analysis of related genes (sox9, bmp4 and Bmp2) during. Metamorphosis[J]. Marine Fisheries, 2009,31(4):337-346.
[35] Ding Y, Yang Y, Chen J, et al. Toxic effects of ZnSe/ZnS quantum dots on the reproduction and genotoxiticy of rare minnow (Gobiocypris rarus)[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2021,247: 109065.
[36] Chen J, Ding Y, Chen H, et al. Reproductive toxicity of InP/ZnS QDs in male rare minnow (Gobiocypris rarus)[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2022,259:109392.
[37] Xu G, Lin G, Lin S, et al. The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization[J]. Sci Rep, 2016,6:37677.
[38] 黄小铭,江星,王川等.量子点在尼罗罗非鱼体内的毒物动力学及组织分布研究[C]//四川省动物学会,重庆市动物学会,贵州省动物学会,云南省动物学会,陕西省动物学会.中国西部动物学学术研讨会论文摘要汇编, 2012:1. Huang X M, Jiang X, Wang C et al. Toxicological dynamics and tissue distribution of quantum dots in Nile Tilapia[C]//Sichuan Zoological Society, Chongqing Zoological Society, Guizhou Zoological Society, Yunnan Zoological Society, Shaanxi Zoological Society. A compilation of abstracts from the Zoological Symposium in Western China, 2012:1.
[39] Yw X, Li L, Wu J, et al. Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice[J]. ACS Appl Bio Mater, 2019,2(10):4193-4201.
[40] Yao Y, Chen Z, Zhang T, et al. Adverse reproductive and developmental consequences of quantum dots[J]. Environ Res, 2022,213:113666.
[41] Gottschalk F, Lassen C, KjoelholtJ J, et al. Modeling flows and concentrations of nine engineered nanomaterials in the Danish environment[J]. Int J Environ Res Public Health, 2015,12(5):5581-5602.
[42] Wang Y, Nowack B. Dynamic probabilistic material flow analysis of nano-SiO₍₂₎, nano iron oxides, nano-CeO₍₂₎, nano-Al₍₂₎O₍₃₎, and quantum dots in seven European regions[J]. Environ Pollut, 2018, 235:589-601.
[43] Giroux M, Zahra Z, Salawu O A, et al. Assessing the environmental effects related to quantum dot structure, function, synthesis and exposure[J]. Environ Sci Nano, 2022,9(3):867-910.
[44] Lewinski N A, Zhu H, Ouyang C R, et al. Trophic transfer of amphiphilic polymer coated CdSe/ZnS quantum dots to Danio rerio[J]. Nanoscale, 2011,3(8):3080-3083.
[45] Ankireddy S R, Kim J. Selective detection of dopamine in the presence of ascorbic acid via fluorescence quenching of InP/ZnS quantum dots[J]. Int J Nanomedicine, 2015,10(Special Issue on diverse applications in Nano-Theranostics):113-119.
[46] Ankireddy S R, Kim J. Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip[J]. Int J Nanomedicine, 2015,10(Special Issue on diverse applications in Nano-Theranostics):121-128.
[47] Yang E, Yao J, Wang L, et al. InP/ZnS quantum dot-based fluorescent probe for directly sensitive and selective detection of horseradish peroxidase[J]. Methods Appl Fluoresc, 2019,7(3):035008.