交替饥饿下PN1/PN2系统抑制NOB研究

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (0 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to inhibit the growth and reproduction of nitrite oxidizing bacteria (NOB), a sequence batch reactor (SBR) (consisting of four groups of reactors R1~R4) was used to run the PN1/PN2 system, in which an alternating starvation/recovery strategy with an alternating 3 day period was used for 70 day operation. The dissolved oxygen concentrations in the starvation phase were set to (1±0.5), (2±0.5), (3±0.5) and (4±0.5) mg/L, respectively, and the selection of alternating cycles and effects of dissolved oxygen conditions on the activity, sludge concentration, particle size and extracellular polymer (EPS) of functional bacteria during the starvation period were evaluated to define the stable operation of the nitrification. The results show that, compared with ammonia oxidizing bacteria (AOB), NOB was more sensitive to starvation, the activity decay rate was higher, and the activity recovery rate of AOB was higher than that of NOB in the first days of the recovery period, so the 3 day alternating cycle could effectively inhibit NOB and retain AOB activity. The nitrous accumulation rate (NAR) of the four groups of reactors reached 73.36%, 84.43%, 91.21% and 95.97%, respectively, followed by sludge reduction, but the sludge concentration of R1~R3 could remain stable after a period of adaptation, while R4 showed a downward trend. The alternating starvation/recovery strategy allowed the system to gradually eliminate flocs with poor sedimentation performance, while the sludge with good sedimentation performance remained in the reactor, and the sludge particle sizes in the four reactors on the 70th day reached 190.69, 197.56, 207.69 and 153.56μm, respectively. Environmental changes stimulated microorganisms to secrete more EPS, so the EPS content of the sludge in the four groups of reactors increased at varying amplitudes. Evidently, the alternating starvation/recovery strategy can stimulate beneficial changes in sludge to achieve effective NOB inhibition and stable NO₂-N accumulation.

Key words： partial nitrification starvation AOB NOB activity inhibition anammox

Received: 05 June 2023

PACS:

X703.5

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Dong
	REN Ji-yuan
	ZHANG Jie

Cite this article:

LI Dong,REN Ji-yuan,ZHANG Jie. Inhibiting NOB under alternating starvation by operating the PN1/PN2 system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 202-210.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I1/202

[1]	Wett B.Development and implementation of a robust deammonification process [J].Water Science & Technology, 2007, 56(7):81-88.
[2]	Wang H, Dan Q, Du R, et al.Enhanced nitrogen removal in partial nitrification-anammox (PNA) suspended sludge system for real municipal wastewater treatment at extremely low carbon to nitrogen ratio [J].Chemical Engineering Journal, 2023,452:139256.
[3]	Bollmann A, Schmidt I, Saunders A M, et al.Influence of starvation on potential ammonia-oxidizing activity and amoA mRNA levels of Nitrosospira briensis [J].Applied and Environmental Microbiology, 2005,71(3):1276-1282.
[4]	Elawwad A, Sandner H, Kappelmeyer U, et al.Long-term starvation and subsequent recovery of nitrifiers in aerated submerged fixed-bed biofilm reactors [J].Environmental Technology, 2013,34(5-8):945-959.
[5]	Liu W L, Yang Q, Ma B, et al.Rapid achievement of nitritation using aerobic starvation [J].Environmental Science & Technology, 2017, 51(7):4001-4008.
[6]	李冬,刘志诚,徐贵达,等.基于间歇饥饿的SNAD工艺运行[J].环境科学, 2020,41(1):337-344.LI D, Liu Z, Xu G, et al.SNAD process operation based on intermittent starvation [J].Environmental Science, 2020,41(1):337-344.
[7]	Kornabos M, Dokianakis S N, Lyberatos G.Partial nitrification/denitrification can be attributed to the slow response of nitrite oxidizing bacteria to periodic anoxic disturbances [J].Environmental Science & Technology, 2010,44(19):7245.
[8]	Salem S, Moussa M S, et al.Determination of the decay rate of nitrifying bacteria [J].Biotechnology and Bioengineering, 2006,94(2):252-262.
[9]	Morgenroth E, Obermayer A, Arnold E, et al.Effect of long-term idle periods on the performance of sequencing batch reactors [J].Water Science & Technology, 2000,41(1):105-113.
[10]	陈佼,陆一新,张建强,等.饥饿对人工快速渗滤系统硝化性能的影响[J].环境科学学报, 2018,38(12):4703-4712.Chen L, Lu Y, Zhang J, et al.Effect of starvation on nitrification performance of artificial rapid diafiltration system [J].Journal of Environmental Science, 2018,38(12):4703-4712.
[11]	李冬,刘名扬,张杰,等.好氧/缺氧交替间歇饥饿CANON工艺处理生活污水[J].哈尔滨工业大学学报, 2022,54(8):11-18.Li D, Liu M, Zhang J, et al.Treatment of domestic sewage by alternating intermittent starvation CANON process of aerobic/anoxic [J].Journal of Harbin Institute of Technology, 2022,54(8):11-18.
[12]	黄勇,黄文慧,顾晓丹,等.城市污水中部分亚硝化-厌氧氨氧化工艺NOB抑制策略的研究进展[J].环境工程学报, 2023,17(4):1075-1083.Huang Y, Huang W, Gu X, et al.Research progress on NOB inhibition strategy of partial nitrosation-anaerobic ammonia oxidation process in municipal sewage [J].Chinese Journal of Environmental Engineering, 2023,17(4):1075-1083.
[13]	高春娣,王惟肖,李浩,等.SBR法交替缺氧好氧模式下短程硝化效率的优化[J].中国环境科学, 2015,35(2):403-409.Gao C, Wang W, Li H, et al.Optimization of short-range nitrification efficiency under alternating hypoxia aerobic mode by SBR method [J].China Environmental Science, 2015,35(2):403-409.
[14]	Wang W, Li D, Li S, et al.Characteristics and formation mechanism of hollow Anammox granular sludge in low-strength ammonia sewage treatment [J].Chemical Engineering Journal, 2021,421:127766.
[15]	何丽金,王少坡,毕艳孟,等.长期饥饿后SPNA微颗粒污泥系统性能恢复[J].中国环境科学, 2021,41(12):5646-5653.He L, Wang S, Bi Y, et al.Performance recovery of SPNA microgranular sludge system after long-term starvation [J].China Environmental Science, 2021,41(12):5646-5653.
[16]	刘文龙,刘超,沈琛,等.活性污泥长期好氧饥饿下的微生物种群结构演化[J].哈尔滨工业大学学报, 2019,51(8):20-27.Liu W, Liu C, Shen C, et al.Evolution of microbial population structure under long-term aerobic starvation of activated sludge [J].Journal of Harbin Institute of Technology, 2019,51(8):20-27.
[17]	李冬,刘名扬,张杰,等.厌氧氨氧化颗粒污泥的长期保藏及快速活性恢复[J].环境科学, 2021,42(6):2957-2965.Li D, Liu M, Zhang J, et al.Long-term preservation and rapid activity recovery of anaerobic ammonia oxidized granular sludge [J].Environmental Science, 2021,42(6):2957-2965.
[18]	Zhang L, Liu M, Zhang S, et al.Integrated fixed-biofilm activated sludge reactor as a powerful tool to enrich anammox biofilm and granular sludge [J].Chemosphere, 2015,140:114-118.
[19]	Vlaeminck S E, Terada A, Smets B F, et al.Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox [J].Applied and Environmental Microbiology, 2010,76(3):900-909.
[20]	Laspidou C S, Rittmann B E.A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass [J].Water Research, 2002,36(11):2711-2720.
[21]	Sutherland I.Biofilm exopolysaccharides:A strong and sticky framework [J].Microbiology, 2001,147(1):3-9.
[22]	李冬,田海成,梁瑜海,等.水质条件对厌氧氨氧化颗粒污泥EPS含量的影响[J].哈尔滨工业大学学报, 2017,49(2):6-12.Li D, Tian H, Liang Y, et al.Effect of water quality conditions on EPS content of anaerobic ammonia oxidation granular sludge [J].Journal of Harbin Institute of Technology, 2017,49(2):6-12.
[23]	罗小娟.不同水力条件对CANON颗粒污泥微生物胞外聚合物的影响[J].环境科技, 2017,30(2):17-22.Luo X.Effects of different hydraulic conditions on microbial extracellular polymers of canon granular sludge [J].Environmental Science and Technology, 2017,30(2):17-22.
[24]	Adav S S, Lee D J, Tay J H.Extracellular polymeric substances and structural stability of aerobic granule [J].Water Research, 2008,42(6/7):1644-1650.
[25]	Li Y, Huang Z, Ruan W, et al.ANAMMOX performance, granulation, and microbial response under COD disturbance [J].Journal of Chemical Technology and Biotechnology, 2015,90(1):10.
[26]	洪小红,张尧超,刘湘伟,等.好氧颗粒污泥颗粒化到稳定性的研究进展[J].广东化工, 2022,49(24):279-282.Hong X, Zhang Y, Liu X, et al.Research progress on granulation to stability of aerobic granular sludge [J].Guangdong Chemical Industry, 2022,49(24):279-282.
[27]	李冬,刘名扬,张杰,等.水力筛分间歇饥饿CANON工艺参数调控及稳定运行[J].哈尔滨工业大学学报, 2022,54(2):1-7.Li D, Liu M, Zhang J, et al.Regulation and stable operation of CANON process parameters for intermittent starvation in hydraulic screening [J].Journal of Harbin Institute of Technology, 2022,54(2):1-7.