纳米银对铜绿微囊藻生物毒性的电荷效应

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Abstract The behavior and biotoxic effects of two silver nanoparticles (AgNPs) modified by polyethyleneimine (BPEI-AgNPs with positive charge) and citric acid (Citrate-AgNPs with negative charge) on Microcystis aeruginosa (M. aeruginosa) were investigated to evaluate the surface charge-dependent biotoxicity of AgNPs on M. aeruginosa. A more pronounced suppressive influence on the growth and photosynthetic activity of M. aeruginosa was observed with the Citrate-AgNPs than with the BPEI-AgNPs. The peak content of malondialdehyde (MDA) in 0.5mg/L Citrate-AgNP group (100.8nmol/mgprot) was higher than that in the same concentration BPEI-AgNP group (82.5nmol/mgprot). Compared with BPEI-AgNPs, Citrate-AgNPs demonstrated a significant up- and down-regulation on sod2 and GPX gene expression in M. aeruginosa, resulting in a higher peroxidation in algal cells. The extracellular polymer (EPS) secretion was observed with BPEI-AgNPs, amounting up to 32.21mg/gSS in 0.5mg/L BPEI-AgNP group (16d). Subsequently, the toxicity of BPEI-AgNPs was neutralized by the coating effect of EPS. The final intracellular Ag⁺ released in the 0.5mg/L Citrate-AgNP group was 2.13 times that of the 0.5mg/L BPEI-AgNP group, and M. aeruginosa cells were seriously ruptured. Therefore, the surge of Ag⁺ ions triggered severe cellular impairment to cells by Citrate-AgNPs. Furthermore, Citrate-AgNPs significantly inhibited the expression of photosynthesis (rbcS and rbcL) and energy metabolism (por) genes.

Key words： silver nanoparticles surface charges Microcystis aeruginosa biotoxicity transcriptomics

Received: 09 June 2023

PACS:

X171.5

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	Articles by authors
	YONG Ling-li
	CHEN You-peng
	GUO Jin-song
	FANG Fang
	SUN Zhuang-zhuang
	YAN Peng

Cite this article:

YONG Ling-li,CHEN You-peng,GUO Jin-song等. Charge effects of silver nanoparticles on biotoxicity of Microcystis aeruginosa[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 510-519.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I1/510

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