不同外源Cd(II)对<i>Pseudomonas aeruginosa</i> EPS的胁迫效应——产量、组分、吸附特性变化及其机制

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Abstract The effects of anions on the yield and characteristics of Pseudomonas aeruginosa EPS and its performance on Cd(II) adsorption were compared by using CdCl₂, CdSO₄ and Cd(NO₃)₂ as stress factors. The results showed that the maximum EPS yield of 214.91mg/g VSS (136.75mg/g VSS in protein content) was observed for Cd(NO₃)₂ at a concentration of 50mg/L, which was 409.31% higher than that before stress/induction. Under this condition, the maximum adsorption capacity for Cd(II) by the EPS was achieved, which was 50% higher than that before stress/induction. HPLC analysis showed that the changes of amino acids in EPS proteins were closely related to the adsorption characteristics of EPS. The results of 3D-EEM, XPS and FTIR showed that the content of C=O, C-N and N-H in EPS increased, which was conducive to the binding of heavy metals. Through competitive adsorption experiments, it was found that EPS has a higher affinity for Cd(II) than Zn(II).

Key words： gram-negative bacteria extracellular polymeric substances protein amino acid competitive adsorption heavy metals

Received: 11 May 2023

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X172

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	Articles by authors
	CHEN Li-yao
	LIAN Ze-yang
	SONG Wei-feng
	DAI Wen-can
	YANG Zuo-yi
	SUN Meng-ge
	HUANG Xiang-wu
	BAI Xiao-yan

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CHEN Li-yao,LIAN Ze-yang,SONG Wei-feng等. Coercive effects of different exogenous Cd(II) on Pseudomonas aeruginosa EPS——Changes in yield, composition, adsorption characteristics and their mechanisms[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 537-547.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I1/537

[1]	Li H M, Wei M, Min W H, et al.Removal of heavy metal Ions in aqueous solution by Exopolysaccharides from Athelia rolfsii [J].Biocatalysis and Agricultural Biotechnology, 2016,6:28-32.
[2]	O'Connell D W, Birkinshaw C, O'Dwyer T F.Heavy metal adsorbents prepared from the modification of cellulose:a review [J].Bioresource Technology, 2008,99(15):6709-6724.
[3]	Lian Z Y, Yang Z Y, Song W F, et al.Effects of different exogenous cadmium compounds on the chemical composition and adsorption properties of two gramnegative bacterial EPS [J].Science of the Total Environment, 2021,806:150511.
[4]	Mota R, Pereira S B, Meazzini M, et al.Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles [J].Journal of proteomics, 2015,120:75-94.
[5]	More T T, Yadav J S S, Yan S, et al.Extracellular polymeric substances of bacteria and their potential environmental applications [J].Journal of Environmental Management, 2014,144:1-25.
[6]	Dash H R, Das S.Interaction between mercuric chloride and extracellular polymers of biofilm-forming mercury resistant marine bacterium Bacillus thuringiensis PW-05[J].RSC Advances, 2016, 6(111):109793-109802.
[7]	Volkel S, Hein S, Benker N, et al.How to cope with heavy metal ions:Cellular and proteome-level stress response to divalent copper and nickel in Halobacterium salinarum R1planktonic and biofilm cells [J].Front Microbiol, 2020,10:3056.
[8]	Zeng W M, Li F, Wu C C, et al.Role of extracellular polymeric substance (EPS) in toxicity response of soil bacteria Bacillus sp.S3to multiple heavy metals [J].Bioprocess and Biosystems Engineering, 2020,43(1):153-167.
[9]	Han J G, Wang S Y, Fan D W, et al.Time-dependent hormetic response of soil alkaline phosphatase induced by Cd and the association with bacterial community composition [J].Microbial Ecology, 2019,78(4):961-973.
[10]	孙梦格,宋卫锋,杨佐毅,等.Cd(Ⅱ)胁迫/诱导下铜绿假单胞菌EPS组分变化及其吸附性能[J].环境科学学报, 2021,41(9):3427-3436.Sun M G, Song W F, Yang Z Y, et al.Effect of Cd(II) stress on the variation in extracellular polymeric substances composition and adsorption performance of Pseudomonas aeruginosa [J].Acta Scientiae Circumstantiae, 2021,41(9):3427-3436.
[11]	Qu C C, Yang S S, Mortimer M, et al.Functional group diversity for the adsorption of lead(Pb) to bacterial cells and extracellular polymeric substances [J].Environmental Pollution, 2022,295:118651.
[12]	黄祥武,宋卫锋,杨佐毅,等.碳源诱导下D.desulfuricans sub sp EPS特性及对Cd(II)的吸附[J].中国环境科学, 2023,43(6):2889-2898.Huang X W, Song W F, Yang Z Y, et al.The EPS characteristics of D.desulfuricans sub sp. and its adsorption performance for Cd(II) under carbon source induction [J].China Environmental Science, 2023, 43(6):2889-2898.
[13]	Lin X Y, Mou R X, Cao Z Y, et al.Characterization of cadmium-resistant bacteria and their potential for reducing accumulation of cadmium in rice grain [J].Science of the Total Environment, 2016b, 569-570:97-104
[14]	连泽阳.不同外源Cd(Ⅱ)胁迫/诱导下Pseudomonas Aeruginosa和Alcaligenes Faecalis EPS特性及其吸附性能研究[D].广州:广东工业大学, 2022.Lian Z Y.Characteristics and adsorption properties of Pseudomonas aeruginosa and Alcaligenes faecalis EPS under different exogenous Cd(II) stress/induction [D].GuangZhou:Guangdong University of Technology, 2022.
[15]	Raunkjær K, Hvitved-Jacobsen T, Nielsen P H.Measurement of pools of protein, carbohydrate and lipid in domestic wastewater [J].Water Research, 1994,28(2):251-262.
[16]	Zhang B, Sun B S, Jin M, et al.Extraction and analysis of extracellular polymeric substances in membrane fouling in submerged MBR [J].Desalination, 2008,227(1):286-294.
[17]	Yude S, Clinkenbeard K D, Clarke C, et al.Pasteurella haemolytica leukotoxin induced apoptosis of bovine lymphocytes involves DNA fragmentation1[J].Veterinary Microbiology, 1999,65(2):153-166.
[18]	Bramhachari P V, Dubey S K.Isolation and characterization of exopolysaccharide produced by Vibrio harveyi strain VB23[J].Letters in Applied Microbiology, 2006,43(5):571-577.
[19]	简静仪,宋卫锋,李海宇,等.锌铜胁迫对Bacillus vallismortis EPS组分变化特征及其吸附性能的影响[J].环境科学学报, 2017,37(6):2099-2106.Jian J Y, Song W F, Li H Y, et al.infuence of the stress of Zn(II) and Cu(II) on component changes and sorption behavior of extracellular polymeric substances (EPS) from Bacillus vallismortis [J].Acta Scientiae Circumstantiae, 2017,37(6):2099-2106.
[20]	Gnanamani A, Kavitha V, Radhakrishnan N, et al.Microbial products (biosurfactant and extracellular chromate reductase) of marine microorganism are the potential agents reduce the oxidative stress induced by toxic heavy metals [J].Colloids and Surfaces B:Biointerfaces, 2010,79(2):334-339.
[21]	Alipanah L, Rohloff J, Winge P, et al.Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum [J].Journal of Experimental Botany, 2015,66(20):6281-6296.
[22]	Izadi P, Izadi P, Eldyasti A.Holistic insights into extracellular polymeric substance (EPS) in anammosx bacterial matrix and the potential sustainable biopolymer recovery:A review [J].Chemosphere, 2021,274:129703.
[23]	Cheah C, Cheow Y L, Ting A S Y.Pre-treatment of exopolymeric substances from bacillus cereus for metal removal as a novel strategy to enhance metal biosorption [J].Water, Air, & Soil Pollution, 2023, 234(2):121.
[24]	Guo X J, He L S, Li Q, et al.Investigating the spatial variability of dissolved organic matter quantity and composition in Lake Wuliangsuhai [J].Ecological Engineering, 2014,62:93-101.
[25]	Ma B R, Li S S, Wang S, et al.Effect of Fe3O4nanoparticles on composition and spectroscopic characteristics of extracellular polymeric substances from activated sludge [J].Process Biochemistry, 2018,75:212-220.
[26]	蒋樟丽,韩福霞,金泽霖,等.粪产碱杆菌AF01产可溶性胞外多糖发酵条件的优化[J].食品工业科技, 2016,37(23):165-169.Jiang Z L, Han F X, Jin Z L, et al.Optimization of fermentation conditions for soluble extracellular polysaccharide production by Alcaligenes Faecalis AF 01[J].Science and Technology of Food Industry, 2016,37(23):165-169.
[27]	连泽阳,杨佐毅,宋卫锋,等.外源Cd(II)胁迫Alcaligenes faecalis过程中阴离子对EPS产量及其特性的影响[J].环境科学学报, 2022, 42(4):81-90.Lian Z Y, Yang Z Y, Song W F, et al.The effect of anions on the yield and characteristics of EPS during the process of exogenous Cd (II) stress of Alcaligenes faecalis [J].Acta Scientiae Circumstantiae, 2022, 42(4):81-90.
[28]	Chia M A, Lombardi A T, da Graça Gama Melão M, et al.Combined nitrogen limitation and cadmium stress stimulate total carbohydrates, lipids, protein and amino acid accumulation in Chlorella vulgaris (Trebouxiophyceae) [J].Aquatic Toxicology, 2015,160:87-95.
[29]	Ishida N, Okubo A, Kawai Hiroyuki, et al.Interaction of amino acids with transition metal ions in solution (i) solution structure of l-lysine with Co(II) and Cu(II) ions as studied by nuclear magnetic resonance spectroscopy [J].Agricultural and Biological Chemistry, 1980,44(2):263-270.
[30]	Mehta S K, Gaur J P.Heavy-metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris [J].New Phytologist, 1999,143(2):253-259.
[31]	Lwalaba J L W, Zvobgo G, Mwamba TM, et al.High accumulation of phenolics and amino acids confers tolerance to the combined stress of cobalt and copper in barley (Hordeum vulagare) [J].Plant Physiology and Biochemistry, 2020,155:927-937.
[32]	Murphy J M, Powell B A, Brumaghim J L.Stability constants of bio-relevant, redox-active metals with amino acids:The challenges of weakly binding ligands [J].Coordination Chemistry Reviews, 2020, 412:213253.
[33]	Effendi A J, Mastroiani L J, Suhardi S H, et al.Potential use of extracellular polymeric substances (EPS) of bacillus subtilis for biosorption of mercury produced from soil-washing effluent.[J].Bioresource Technology Reports, 2023,22:101481.
[34]	Yin Y R, Hu Y Y, Xiong F.Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus [J].International Biodeterioration & Biodegradation, 2011,65(7):1012-1018.
[35]	Di Franco F, Tranchida G, Pupillo D, et al.Effect of E.coli biofilm formation and removal on passive films on AISI 316L during fermentation processes [J].Corrosion Science, 2021,185:109430.
[36]	Wei L L, Xia X H, Zhu F Y, et al.Dewatering efficiency of sewage sludge during Fe2+-activated persulfate oxidation:Effect of hydrophobic/hydrophilic properties of sludge EPS [J].Water Research, 2020,181:115903.
[37]	Shou W J, Kang F X, Lu J H.Nature and value of freely dissolved EPS ecosystem services:Insight into molecular coupling mechanisms for regulating metal toxicity [J].Environmental Science and Technology, 2018,52(2):457-466.
[38]	Zhao W Q, Walker S L, Huang Q Y, et al.Contrasting effects of extracellular polymeric substances on the surface characteristics of bacterial pathogens and cell attachment to soil particles [J].Chemical Geology, 2015,410:79-88.
[39]	Hou X L, Liu S T, Zhang Z T.Role of extracellular polymeric substance in determining the high aggregation ability of anammox sludge [J].Water Research, 2015,75:51-62.
[40]	Rashmi V, Darshana A, Bhuvaneshwari T, et al.Uranium adsorption and oil emulsification by extracellular polysaccharide (EPS) of a halophilic unicellular marine cyanobacterium Synechococcus elongatus BDU130911[J].Current Research in Green and Sustainable Chemistry, 2021,4:100051.
[41]	Badireddy A R, Chellam S, Gassman P L, et al.Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions [J].Water Research, 2010,44(15):4505-4516.
[42]	Shi Y J, Liu Y.Evolution of extracellular polymeric substances (EPS) in aerobic sludge granulation:Composition, adherence and viscoelastic properties [J].Chemosphere, 2021,262:128033.
[43]	Li K, Wei D, Yan T, et al.Responses of soluble microbial products and extracellular polymeric substances to the presence of toxic 2,6-dichlorophenol in aerobic granular sludge system [J].Journal of Environmental Management, 2016,183:594-600.
[44]	Jiang W, Saxena A, Song B, et al.Elucidation of functional groups on gram-positive and gram-negative bacterial surfaces using infrared spectroscopy [J].Langmuir, 2004,20(26):11433-11442.
[45]	周睿,周雅琪,王丽丽,等.一株钒还原菌的分离鉴定及V(V)还原机理研究[J].中国环境科学, 2023,43(6):2926-2937.Zhou R, Zhou Y Q, Wang L L, et al.Isolation and identification of a vanadium reducing bacterium and the mechanism of V(V) bioreduction [J].China Environmental Science, 2023,43(6):2926-2937.
[46]	Yan S J, Cai Y g, Li H q, et al.Enhancement of cadmium adsorption by EPS-montmorillonite composites [J].Environmental Pollution, 2019,252:1509-1518.
[47]	Xu S Q, Jiang Q.Surface modification of carbon fiber support by ferrous oxalate for biofilm wastewater treatment system [J].Journal of Cleaner Production, 2018,194:416-424.
[48]	Lin D, Ma W T, Jin Z X, et al.Interactions of EPS with soil minerals:A combination study by ITC and CLSM [J].Colloids and Surfaces B, 2016,138:10-16.
[49]	Szcześ A, Czemierska M, Jarosz-Wilkołazka A.Calcium carbonate formation on mica supported extracellular polymeric substance produced by Rhodococcus opacus [J].Journal of Solid State Chemistry, 2016,242:212-221.
[50]	Yang Y, Wikieł A J, Dall'Agnol L T, et al.Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures [J].Biofouling, 2016,32(1):95-108.
[51]	辛梅芬.肠杆菌胞外聚合物对镉铅吸附特性研究[D].桂林:广西师范大学, 2022.Xin M F.Research on Biosorption Characteristics of Cadmium and Lead by Extracellular Polymeric Substances from Enterobacter sp.[D].GuiLin:Guangxi Normal University, 2022.
[52]	Ye S H, Zhang M P, Yang H, et al.Biosorption of Cu2+, Pb2+ and Cr6+ by a novel exopolysaccharide from Arthrobacter ps-5[J].Carbohydr Polym, 2014,101:50-56.
[53]	Yin Y R, Hu Y Y, Xiong F.Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus [J].International Biodeterioration & Biodegradation, 2011,65(7):1012-1018.
[54]	Yang J X, Wei W, Pi S S, et al.Competitive adsorption of heavy metals by extracellular polymeric substances extracted from Klebsiella sp.J1[J].Bioresource Technology, 2015,196:533-539.