表观气速对除磷污泥颗粒化性能及代谢特征的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2010 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract In this study, the effect of superficial gas velocity on the granulation and metabolic characteristics of biological phosphorus removal sludge was investigated using a mixture of 60% sodium acetate and 40% soluble starch as the carbon source. The results showed that, unlike under the condition of small-molecule carbon source, the granulation of biological phosphorus removal sludge under complex carbon source condition was determined by both shear force and intracellular storage of carbon source, and the granulation process of the sludge was not completely consistent with the magnitude of the superficial gas velocity. R1 with the largest superficial gas velocity (0.45cm/s) achieved granulation on day 110, and R3 with the smallest superficial gas velocity (0.15cm/s) achieved granulation on day 190, which was slower than that in R1 but faster than that in R2 (0.30cm/s). Decreasing the superficial gas velocity successively increased the content of filamentous bacteria and decreased the hydrophobicity of the tightly bound extracellular polymeric substance (TB-EPS); but the system was easier to meet the demand of anaerobic feast and aerobic famine, and the content of polyhydroxyalkanoates (PHAs) from anaerobic synthesis increased, and the corresponding content in R1, R2, R3 was 2.28, 8.16 and 23.96mg/gVSS, respectively. High-throughput sequencing analysis of 16S rRNA genes showed that as the superficial gas velocity decreased, the abundances of Chloroflexi with hydrolysis and fermentation function successively increased, which were 7.86%, 8.77% and 32.74%, respectively. At the genus level, Candidatus_Competibacter, a typical glycogen accumulating organism, was the key group for sludge granulation. The high superficial gas velocity in R1was beneficial to the maintenance and proliferation of this group, and the granulation process in R1 was the fastest. The superficial gas velocity in R3 was the lowest, and Kouleothrix (filamentous bacterium type 1851, belonging to the Chloroflexi) with the function of hydrolysis and fermentation thrived (10.44% in abundance), which promoted the proliferation of Candidatus_Competibacter (25.73% in abundance) by providing filamentous skeleton and small-molecule carbon source, thus accelerating the granulation of the sludge.

Key words： complex carbon source granular sludge superficial gas velocity intracellular storage bacterial community structure effect

Received: 07 July 2023

PACS:

X703.5

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Xi
	HU Bin
	ZHANG Rui-feng
	GONG Yan-zhe
	CAI Hu-lin
	CAO Yin-huan
	YANG Wen-hao
	MU Rui-hua

Cite this article:

CHEN Xi,HU Bin,ZHANG Rui-feng等. Effect of superficial gas velocity on the granulation and metabolic characteristics of phosphorus removal sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(2): 686-698.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I2/686

[1]	Pronk M, Giesenb A, Thompson A, Robertson S, et al. Aerobic granular biomass technology:advancements in design, applications and further developments [J]. Water Practice & Technology, 2017, 12(4):987-996.
[2]	Shin HS, Lim KH, Park HS. Effect of shear stress on granulation in oxygen aerobic upflow sludge bed reactors [J]. Water Science & Technology, 1992,26(3/4):601-605.
[3]	Chen Y, Jiang W, Liang DT, et al. Structure and stability of aerobic granules cultivated under different shear force in sequencing batch reactors [J]. Applied Microbiology and Biotechnology, 2007,76(5):1199-1208.
[4]	Tay JH, Liu Q, Liu Y. The effects of shear force on the formation, structure and metabolism of aerobic granules [J]. Applied Microbiology and Biotechnology, 2001,57:227-233.
[5]	王陆玺,周楠,王晨旭,等.流体流速对好氧颗粒污泥快速培养的影响[J]. 中国环境科学, 2018,38(6):2090-2096. Wang L X, Zhou N, Wang C X, et al. Effect of fluid flow rate on rapid cultivation of aerobic granular sludge [J]. China Environmental Science, 2018,38(6):2090-2096.
[6]	de Kreuk M K, Heijnen J J, van Loosdrecht MCM. Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge [J]. Biotechnology and Bioengineering, 2005,90(6):761-769.
[7]	de Kreuk M K, van Loosdrecht M C. Selection of slow growing organisms as a means for improving aerobic granular sludge stability [J]. Water Science and Technology, 2004,49(11/12):9-17.
[8]	Layer M, Bock K, Ranzinger F, et al. Particulate substrate retention in plug-flow and fully-mixed conditions during operation of aerobic granular sludge systems [J]. Water Research X, 2020,9:100075.
[9]	Layer M, Adler A, Reynaert E, et al. Organic substrate diffusibility governs microbial community composition, nutrient removal performance and kinetics of granulation of aerobic granular sludge [J]. Water Research X, 2019,4:100033.
[10]	陈希,袁乙卜,张建民,等.大分子有机物对除磷颗粒污泥特性及菌群结构的影响[J]. 环境科学学报, 2021,41(4):1309-1322. Chen X, Yuan Y B, Zhang J M, et al. Effects of macromolecular organic matters on the characteristics and bacterial community structure of the phosphorus removal granular sludge [J]. Acta Scientiae Circumstantiae, 2021,41(4):1309-1322.
[11]	国家环境保护总局.水和废水分析检测方法[M]. 4版.北京:中国环境科学出版社, 2002. State Environmental Protection Administration."Analysis and detection methods of water and wastewater"[M]. Version 4. BeiJing:China Environmental Science Press, 2002.
[12]	Angela M, Béatrice B, Mathieu S. Biologically induced phosphorus precipitation in aerobic granular sludge process [J]. Water Research, 2011,45(12):3776-3786.
[13]	温丹丹,袁林江,陈希,等.3种不同工艺切换下活性污泥菌群结构及代谢产物对污泥沉降性能的影响[J]. 环境科学, 2018,39(10):4644-4652. Wen D D, Yuan L J, Chen X, et al. Effect of microbial community structure and metabolites on sludge settling ability under three different switching condition processes [J]. Environmental Science, 2018,39(10):4644-4652.
[14]	Hou X, Liu S, Zhang Z. Role of extracellular polymeric substance in determining the high aggregation ability of anammox sludge [J]. Water Research, 2015,75:51-62.
[15]	诸葛健,王正祥.《工业微生物实验技术手册》[M]. 北京:中国轻工业出版社, 1994:213-214. Zhu G J, Wang Z X. "Technical manual for industrial microbiology experiments"[M]. China Light Industry Press, 1994:213-214.
[16]	高庆,印建和,穆华容,等.对羟基联苯法测定啤酒中乳酸[J]. 酿酒, 2005,32(6):93-95. Gao Q, Yin J H, Mu H R, et al. Determination of lactic acid in beer by p-hydroxybiphenyl method [J]. Winemaking, 2005,32(6):93-95.
[17]	Oehmen A, Keller-Lehmann B, Zeng R J, et al. Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J]. Journal of Chromatography A, 2005,1070(1/2):131-136.
[18]	王琪,李冬,李鹏垚,等.厌/缺氧时间对好氧颗粒污泥同步硝化内源反硝化和除磷的影响[J]. 中国环境科学, 2022,42(9):4199-4206. Wang Q, Li D, Li P Y, et al. Effect of anaerobic/anoxic time on simultaneous nitrification endogenous denitrification and phosphorus removal of aerobic granular sludge [J]. China Environmental Science, 2022,42(9):4199-4206.
[19]	由阳,彭永臻,王淑莹,等.强化生物除磷系统胞内聚合物测定方法优化[J]. 哈尔滨工业大学学报, 2010,42(2):207-211. You Y, Peng Y Z, Wang S Y, et al. Optimization of intracellular polymer analysis for enhanced biological phosphorous removal system [J]. Journal of Harbin Institute of Technology, 2010,42(2):207-211.
[20]	万琼,安少锋,鲁海峰,等.城市污水处理厂活性污泥系统仙女虫的产生与防治[J]. 中国给水排水, 2011,27(15):1-3,8. Wan Q, An S F, Lu H F, et al. Production,prevention and cure of nais elinguis in activated sludge system of WWTP [J]. China Water & Wastewater, 2011,27(15):1-3,8.
[21]	van Dijk EJH, Haaksman VA, van Loosdrecht MCM, et al. On the mechanisms for aerobic granulation-model based evaluation [J]. Water Research, 2022,216:118365.
[22]	Liu Y, Tay J. The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge [J]. Water Research, 2002, 36(7):1653-1665.
[23]	Beun J J, van Loosdrecht M C M, Heijnen J J. Aerobic granulation in a sequencing batch airlift reactor [J]. Water Research, 2002,36(3):702-712.
[24]	Yang Z, Bin L, Huang S, et al. Revealing the stability of aerobic granular sludge in a membrane bioreactor under different DO values by proteomics analysis [J]. Bioresource Technology Reports, 2021, 14:100673.
[25]	周赟成.曝气强度和反应器高径比对好氧颗粒污泥稳定性影响研究[D]. 杭州:浙江工业大学, 2019. Zhou Y C. Determining the effects of aeration intensity and reactor height to diameter ratio on granule stability analysis [D]. Hangzhou:Zhejiang University of Technology, 2019.
[26]	Lopez-Vazquez C M, Oehmen A, Hooijmans C M, et al. Modeling the PAO-GAO competition:effects of carbon source, pH and temperature [J]. Water Research, 2009,43(2):450-462.
[27]	李冬,李悦,杨敬畏,等.提高SBR好氧颗粒污泥脱氮除磷性能的策略调控[J]. 中国环境科学, 2022,42(10):4581-4587. Li D, Li Y, Yang J W, et al. Strategy regulation to improve nitrogen and phosphorus removal performance of SBR aerobic granular sludge [J]. China Envirommental Science, 2022,42(10):4581-4587.
[28]	Zhang L, Feng X, Zhu N, et al. Role of extracellular protein in the formation and stability of aerobic granules [J]. Enzyme and Microbial Technology, 2007,41(5):551-557.
[29]	Tay J H, Liu Q S, Liu Y. The role of cellular polysaccharides in the formation and stability of aerobic granules [J]. Letters in Applied Microbiology, 2001,33(3):222-226.
[30]	Sun Y W, Angelotti B, Brooks M, Wang Z W. Feast/famine ratio determined continuous flow aerobic granulation [J]. Science of the Total Environment, 2021,750:141467.
[31]	Luo D, Yuan L, Liu L, et al. The mechanism of biological phosphorus removal under anoxic-aerobic alternation condition with starch as sole carbon source and its biochemical pathway [J]. Biochemical Engineering Journal, 2018,132:90-99.
[32]	Haaksman V A, Mirghorayshi M, van Loosdrecht M C M, et al. Impact of aerobic availability of readily biodegradable COD on morphological stability of aerobic granular sludge [J]. Water Research, 2020,187:116402.
[33]	Zhou J H, Ren Q, Xu X L, et al. Enhancing stability of aerobic granules by microbial selection pressure using height-adjustable influent strategy [J]. Water Research, 2021,117356.
[34]	Hu T T, Peng Y Z, Yuan C S, et al. Enhanced nutrient removal and facilitating granulation via intermittent aeration in simultaneous partial nitrification endogenous denitrification and phosphorus removal (SPNEDpr) process [J]. Chemosphere, 2021,285,131443.
[35]	Zou J, Pan J, Wu S, et al. Rapid control of activated sludge bulking and simultaneous acceleration of aerobic granulation by adding intact aerobic granular sludge [J]. Science of the Total Environment, 2019, 674:105-113.
[36]	Liu J, Li J, Wang X, et al. Rapid aerobic granulation in an SBR treating piggery wastewater by seeding sludge from a municipal WWTP [J]. Journal of Environmental Sciences, 2017,51:332-341.
[37]	Bovio-Winkler P, Guerrero L D, Erijman L, et al. Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors [J]. BMC Microbiology, 2023,23(1):45.
[38]	McIlroy S J, Albertsen M, Andresen E K, et al. ‘Candidatus Competibacter’-lineage genomes retrieved from metagenomes reveal functional metabolic diversity [J]. The Isme Journal, 2014,8(3):613-624.
[39]	Seviour T W, Lambert L K, Pijuan M, et al. Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus "Competibacter phosphatis" [J]. Applied Microbiology and Biotechnology, 2011,92(6):1297-1305.
[40]	Wang L, Yu X, Xiong W, et al. Enhancing robustness of aerobic granule sludge under low C/N ratios with addition of kitchen wastewater [J]. Journal of Environmental Management, 2020,265:110503.
[41]	Kristiansen R, Nguyen H T T, Saunders AM, et al. A metabolic model for members of the genus tetrasphaera involved in enhanced biological phosphorus removal [J]. The ISME Journal, 2013,7(3):543-554.
[42]	Seviour R J, Maszenan A M, Soddell J A, et al. Microbiology of the ‘G-bacteria’ in activated sludge:minireview [J]. Environmental Microbiology, 2000,2(6):581-593.
[43]	McIlroy S J, Speirs L B M, Tucci J, et al. In situ profiling of microbial communities in full-scale aerobic sequencing batch reactors treating winery waste in australia [J]. Environmental Science & Technology, 2011,45:8794-8803.
[44]	Falvo A, Levantesi C, Rossetti S. Synthesis of intracellular storage polymers by Amaricoccus kaplicensis, a tetrad forming bacterium present in activated sludge [J]. Journal of Applied Microbiology, 2001,91(2):299-305.
[45]	Tian X, Shen Z, Zhou Y, et al. Inhibition on biological acidification and microbial community by high-strength acetaldehyde [J]. Process Safety and Environmental Protection, 2020,143:231-238.
[46]	Zhang T, Wang B, Li X, et al. Achieving partial nitrification in a continuous post-denitrification reactor treating low C/N sewage [J]. Chemical Engineering Journal, 2018,335:330-337.
[47]	Kohno T, Sei K, Mori K. Characterization of type 1851 organism isolated from activated sludge samples [J]. Water Science and Technology, 2002,46(1/2):111-114.