不同补料方式对好氧颗粒污泥合成PHA的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2362 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To enhance the production of polyhydroxyalkanoates (PHA) by aerobic granular sludge (AGS), the impact of four different extra dosing strategies (no-dosing, aerobic 0h-dosing, aerobic 0.5h-dosing, and aerobic 1h-dosing) on the synthesis of PHA by AGS were investigated. Simultaneously, the removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN) and solubility orthophosphate (SOP) by AGS, along with changes in the microbial community composition were examined. The results indicate that extra dosing of organic carbon positively influenced PHA production, with the highest yield (125.06mg/g) observed under aerobic 1h-dosing condition. Additionally, the nutrient removal efficiency of AGS was influenced by dosing time. When dosing was postponed from aerobic 0h to 1h, TN removal decreased from (87.49 ± 5.49)% to (67.60 ± 16.24)%, and the removal efficiency of SOP decreased from (94.08 ± 1.42)% to (62.91 ± 15.33)%. Notably, under various dosing conditions, the microbial community structure of AGS exhibited significant variation. The dominant bacteria under no-dosing and aerobic 0h-dosing conditions were Acinetobacter, capable of both PHA production and nutrients removal. Conversely, while under aerobic 1h-dosing conditions, Hydrogenophaga and Flavobacterium were the dominant bacteria, specializing in PHA production.

Key words： aerobic granular sludge PHA nitrogen and phosphorus removal dosing time effect

Received: 19 July 2023

PACS:

X703.5

Corresponding Authors: 孙迎雪,教授,sunyx@th.btbu.edu.cn E-mail: sunyx@th.btbu.edu.cn

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Song
	YUAN Quan
	LOU Yu-qing
	ZHANG Yan-ping
	SUN Ying-xue

Cite this article:

CHEN Song,YUAN Quan,LOU Yu-qing等. Effect of different extra dosing methods on the production of PHA from aerobic granular sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1286-1295.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I3/1286

[1] 杨林,李夏桐,孙卫宁,等.VFAs组分调控对合成PHA影响研究进展[J]. 应用化工, 2023,52(9):2651-2654,2659. Yang L, Li X, Li X T, Sun X N, et al. Progress in the influence of VFAs component regulation on synthetic PHA[J]. Applied Chemical Industry, 2023,52(9):2651-2654,2659.
[2] Sirohi R, Prakash Pandey J, Kumar Gaur V, et al. Critical overview of biomass feedstocks as sustainable substrates for the production of polyhydroxybutyrate (phb)[J]. Bioresource Technology, 2020,311:123536.
[3] 孟栋,李枘枘,刘玉玲,等.利用剩余活性污泥合成聚羟基脂肪酸酯的研究进展[J]. 生物工程学报, 2019,35(11):2165-2176. Meng D, Li R R, Liu Y L, et al. Advances in synthesis of polyhydroxyalkanoates by using residual activated sludge[J]. Chin J Biotech, 2019,35(11):2165-2176.
[4] 刘泽清,宋秀兰.有机废料合成聚羟基脂肪酸酯的研究进展[J]. 应用化工, 2022,51(7):2113-2120. Liu Z Q, Song X L. Research progress of polyhydroxybutyrate(PHA) synthesis using various organic waste streams[J]. Applied Chemical Industry, 2022,51(7):2113-2120.
[5] Zhou W, Bergsma S, Colpa D I, et al. Polyhydroxyalkanoates (phas) synthesis and degradation by microbes and applications towards a circular economy[J]. Journal of Environmental Management, 2023, 341:118033.
[6] Isern-Cazorla L, Mineo A, Suárez-Ojeda M E, et al. Effect of organic loading rate on the production of polyhydroxyalkanoates from sewage sludge[J]. Journal of Environmental Management, 2023,343:118272.
[7] Simona C, Laura L, Francesco V, et al. Effect of the organic loading rate on the pha-storing microbiome in sequencing batch reactors operated with uncoupled carbon and nitrogen feeding[J]. Science of The Total Environment, 2022,825:153995.
[8] Frison N, Andreolli M, Botturi A, et al. Effects of the sludge retention time and carbon source on polyhydroxyalkanoate-storing biomass selection under aerobic-feast and anoxic-famine conditions[J]. ACS Sustainable Chemistry & Engineering, 2021,9(28):9455-9464.
[9] Alaux E, Marie B, Couvreur M, et al. Impact of phosphorus limitation on medium-chain-length polyhydroxyalkanoate production by activated sludge[J]. Applied Microbiology and Biotechnology, 2023, 107(11):3509-3522.
[10] 李小虎,薛鹏程,孙佳佳.活性污泥胞内聚合物的合成影响因素初探[J]. 环境与发展, 2017,29(10):127-128,130. Li X H, Xue P C, Sun J J. Preliminary study on synthesis factors of intracellular polymeric substance in activated sludge[J]. Environment and Development, 2017,29(10):127-128,130.
[11] 冉依禾,郭亮,刘一平,等.不同比例乙酸和丙酸对活性污泥微生物合成聚羟基脂肪酸酯的影响[J]. 环境工程学报, 2017,11(2):1276- 1280. Ran Y H, Guo L, Liu Y P, et al. Effect of different ratios of acetic acid and propionic acid on microbial polyhydroxyalkanoate synthesis with activated sludge[J]. Chinese Journal of Environmental Engineering, 2017,11(2):1276-1280.
[12] Valentino F, Moretto G, Lorini L, et al. Pilot-scale polyhydroxyalkanoate production from combined treatment of organic fraction of municipal solid waste and sewage sludge[J]. Industrial & Engineering Chemistry Research, 2019,58(27):12149-12158.
[13] De Sousa Rollemberg S L, Mendes Barros A R, Milen Firmino P I, et al. Aerobic granular sludge:cultivation parameters and removal mechanisms[J]. Bioresource Technology, 2018,270:678-688.
[14] Nancharaiah Y V, Kiran Kumar Reddy G. Aerobic granular sludge technology:mechanisms of granulation and biotechnological applications[J]. Bioresource Technology, 2018,247:1128-1143.
[15] 杨国靖,李小明,曾光明.SBR系统同步脱氮除磷好氧颗粒污泥的培养[J]. 中国给水排水, 2008,(9):33-37. Yang G J, Li X M, Zeng G M. Cuitivation of Aerobic Granular Sludge for Simultaneous Nitrogen and Phosphorus Removal in SBR System[J] China Water & Wastewater, 2008,(9):33-37.
[16] Laanbroek H J, Gerards S. Competition for limiting amounts of oxygen between nitrosomonas europaea and nitrobacter winogradskyi grown in mixed continuous cultures[J]. Archives of Microbiology, 1993,159(5):453-459.
[17] 王杰,彭永臻,杨雄,等.不同碳源种类对好氧颗粒污泥合成PHA的影响[J]. 中国环境科学, 2015,35(8):2360-2366. Wang J, Peng Y Z, Yang X, et al. Effect of various types carbon source on the synthesis of PHA of aerobic granular sludge[J]. China Environmental Science, 2015,35(8):2360-2366.
[18] Rojas-Zamora U, Fajardo-Ortiz M D C, Cuetero-Martínez Y, et al. Aerobic granulation for polyhydroxyalkanoates accumulation using organic waste leachates[J]. Journal of Water Process Engineering, 2023,51:103464.
[19] 刘展华.过硫酸氢钾复合盐耦合污泥碱性发酵获取碳源合成PHA[D]. 太原:太原理工大学, 2021. Liu Z H, Synthesis of PHAs using Carbon Source from alkaline fermentation of sludge coupled with potassium perbisulfate compound salt[D]. Taiyuan:Taiyuan University of Technology, 2021.
[20] 张杰,杨杰,李冬,等.AOA-O模式下好氧颗粒污泥同步硝化内源反硝化除磷[J]. 中国环境科学, 2023,43(10):5226-5234. Zhang J, Yang J, Li D, et al. Simultaneous nitrification and phosphorus removal of aerobic granular sludge in AOA-O mode[J]. China Environmental Science, 2023,43(10):5226-5234.
[21] 易名儒,曾玉,刘永,等.不同粒径好氧颗粒污泥的结构稳定性及污染物去除效果[J]. 环境科技, 2021,34(5):23-28. Yi M R, Zeng Y, Liu Y, et al. Structural Stability and Contaminant Removal Efficiency of Aerobic Granular Sludge with Different Particle Size[J]. Environmental Science and Technology, 2021,34(5):23-28.
[22] 王昌稳,赵白航,李军,等.好氧颗粒污泥吸附氨氮性能[J]. 化工学报, 2014,65(3):942-947. Wang C W, Zhao B H, Li J, et al. Performance of ammonia adsorption by aerobic granular sludge[J]. CIESC Journal, 2014,65(3):942-947.
[23] 谢一涵.基于限氧段补料的活性污泥合成PHAs效能研究[D]. 广州:广州大学, 2021. Xie Y H, Study on the efficacy of activated sludge synthesis of PHAs based on oxygen limited feedstock[D]. Guangzhou:Guangzhou University, 2021.
[24] 张艳萍,秦清.活性污泥利用混合碳源合成PHAs的工艺条件优[J]. 环境科学与技术, 2015,38(6P):269-272. Zhang Y P, Qin Q, Optimal process conditions for activated sludge synthesizing polyhydroxyalkanoates using mixed carbon sources of acetic and propionic acid[J]. Environmental Science & Technology, 2015,38(6P):269-272.
[25] Gobi K, Vadivelu V M. Dynamics of polyhydroxyalkanoate accumulation in aerobic granules during the growth-disintegration cycle[J]. Bioresource Technology, 2015,196:731-735.
[26] 陈玮,陈志强,温沁雪,等.SBR启动方式对活性污泥合成PHA的影响[J]. 中国给水排水, 2012,28(15):85-88. Chen W, Chen Z Q, Wen Q X, et al. Influence of start-up mode of SBR on synthesis of polyhydroxyalkanoate by activated sludge[J]. China Water & Wastewater, 2012,28(15):85-88.
[27] Zheng Y, Guo L, Liu Y, et al. Effects of chemical oxygen demand concentration, ph and operation cycle on polyhydroxyalkanoates synthesis with waste sludge[J]. Environmental Technology, 2021, 42(12):1922-1929.
[28] Liao Q, Guo L, Ran Y, et al. Optimization of polyhydroxyalkanoates (pha) synthesis with heat pretreated waste sludge[J]. Waste Management, 2018,82:15-25.
[29] Gobi K, Vadivelu V M. Aerobic dynamic feeding as a strategy for in situ accumulation of polyhydroxyalkanoate in aerobic granules[J]. Bioresource Technology, 2014,161:441-445.
[30] 黄龙.产PHA优势菌群富集机制与三段式混菌工艺优化研究[D]. 哈尔滨:哈尔滨工业大学, 2018. Huang L. Enrichment Mechanism of PHA Producing Dominant Bacteria and Optimization of Three-stage Mixed Culture Process[D]. Harbin:Harbin Institute of Technology, 2018.
[31] 衣凤聪.C/N非耦合强化产PHA富集工艺优化及其代谢机理研究[D]. 哈尔滨:哈尔滨工业大学, 2022. Yi F C. Study on C/N uncouple process for enhancing PHA enrichment and metabolism mechanism[D]. Harbin:Harbin Insititute of Technology, 2022.
[32] 黄惠珺,王淑莹,王中玮,等.不同碳源类型对活性污泥PHA贮存及转化的影响[J]. 化工学报, 2010,61(6):1510-1515. Huang H J, Wang S Y, Wang Z W, et al. Effect of various types of carbon source on biochemical storage and substrate transformation of activated sludge[J]. CIESC Journal, 2010,61(6):1510-1515.
[33] 秦清,张艳萍.3种短链脂肪酸对活性污泥储存PHA的影响[J]. 环境工程学报, 2014,8(7):2859-2864. Qin Q, Zhang Y P, et al. Impact of three short-chain fatty acids on PHA storage by activated sludge[J]. Chinese Journal of Environmental Engineering, 2014,8(7):2859-2864.
[34] 刘煜.活性污泥合成PHA的工艺条件优化[D]. 广州:广州大学, 2020. Liu Y, Optimization of process conditions for PHA synthesis from activated sludge[D]. Guangzhou:Guangzhou University, 2020.
[35] Yan S, Tyagi R D, Surampalli R Y. Polyhydroxyalkanoates (pha) production using wastewater as carbon source and activated sludge as microorganisms[J]. Water Science and Technology:A Journal of the International Association on Water Pollution Research, 2006,53(6):175-180.
[36] Morgan-Sagastume F. Characterisation of open, mixed microbial cultures for polyhydroxyalkanoate (pha) production[J]. Reviews in Environmental Science and Bio/Technology, 2016,15(4):593-625.
[37] 侯怡文.菌藻共生颗粒污泥形成过程中的群体感应效应及微生物群落演替规律[D]. 西安:西安建筑科技大学, 2022. Hou Y W. Quorum induction effect and succession rule of microbial community during the formation of algal-bacterial granular sludge[D]. Xian:Xian University of Architecture and Technology, 2022.
[38] 姜鹏,曾小伦,邹嘉豪,等.产聚羟基脂肪酸酯的活性污泥驯化及细菌群落结构研究[J]. 环境科学与技术, 2020,43(10):85-89. Jiang P, Zeng X L, Zou J H, et al. Enrichment of polyhydroxyalkanoate-accumulating microorganisms in activated sludge and analysis of bacterial community structure[J]. Environmental Science & Technology, 2020,43(10):85-89.
[39] 袁野,周佳,屈建航,等.高效反硝化聚磷菌的筛选及其脱氮除磷条件和性能研究[J]. 生物技术通报, 2023,39(7):266-276. Yuan Y, Zhou J, Qu J H, et al. Screening of an efficient denitrifying phosphorus-accumulating bacterium and its denitrification and phosphorus removal[J]. Biotechnology Bulletin, 2023,39(7):266- 276.
[40] Colpa D I, Zhou W, Wempe J P, et al. Thauera aminoaromatica mz1t identified as a polyhydroxyalkanoate-producing bacterium within a mixed microbial consortium[J]. Bioengineering (Basel, Switzerland), 2020,7(1):19.
[41] 徐少娟,孙培德,郑雄柳,等.复合底物对颗粒化EBPR系统除磷特性的影响及优化试验研究[J]. 环境科学, 2011,31(4):745-751. Xu S J, Sun P D, Zheng X L, et al. Effects of Composite Substrates on the Phosphorus Removal in Granule-based EBPR System and Its Optimization Experiment[J]. Environmental Science, 2011,31(4):745-751.