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| Effect of temperature and load variation of pda system initiated by anammox bacteria self-enrichment |
| MA Yu-qing, WANG Bo, LI Xiao-di |
| Engineering Research Center of Beijing, National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China |
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Abstract A UASB inoculated with PDA sludge initiated by anammox bacteria self-enrichment was used to gradually increase the influent nitrogen concentration from 20mg/L to 100mg/L at 25°C down to 19°C (the nitrogen load increased from 3.2g/(m3·d) to 4.0g/(m3·d)). The reactor was operated for 115 days with a maximum total nitrogen removal efficiency of 89.9%. 16S rRNA gene sequencing results showed that Candidatus Brocadia as the main anammox functional bacterium reached 3.7% abundance in the bio-carriers and the partial denitrifying functional bacterium Thaurea reached a maximum abundance of 15.7% in the floc sludge. Metagenomic analysis showed that the genes Nar and Nap, associated with partial denitrification, and Nir, Hzs and Hdh, associated with anammox, were fully expressed.
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Received: 12 July 2023
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Corresponding Authors:
王博,副教授,wbo@bjut.edu.cn
E-mail: wbo@bjut.edu.cn
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