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Mechanism and regulation of N2O production and elimination in Anammox-DAMO system under the influence of temperature |
HAN Meng-ru, LOU Ju-qing, XU Fan |
College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China |
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Abstract The Anammox-DAMO system was used to investigate the effects of temperature on the system performance and N2O production and consumption. The enzymatic kinetics model of the process was established. The results showed that the system performance deteriorated significantly and more N2O was accumulated under the high temperature stress(40°C). The abundance of Acidovorax and Thauera genera decreased significantly under high temperature conditions, while the abundance of Bacillus genera increased. Inhibition of N2O reductase activity was the main cause of the increase in N2O emissions. The enzyme kinetics results indicated that N2O emission reduction can be maximized at 35°C.
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Received: 25 July 2023
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Corresponding Authors:
楼菊青,教授,ljq7393@163.com
E-mail: ljq7393@163.com
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[1] Lee H S, Tang Y, Rittmann B E, et al. Anaerobic oxidation of methane coupled to denitrification:fundamentals, challenges, and potential[J]. Critical Reviews In Environmental Science and Technology, 2018,48(19-21):1067-1093. [2] Hu B L, Shen L D, Lian X, et al. Evidence for nitrite-dependent anaerobic methane oxidation as a previously overlooked microbial methane sink in wetlands[J]. Proceedings of the National Academy of Sciences, 2014,111(12):4495-4500. [3] Shen L D, Liu S, He Z F, et al. Depth-specific distribution and importance of nitrite-dependent anaerobic ammonium and methane- oxidising bacteria in an urban wetland[J]. Soil Biology and Biochemistry, 2015,83:43-51. [4] Deutzmann J S, Stief P, Brandes J, et al. Anaerobic methane oxidation coupled to denitrification is the dominant methane sink in a deep lake[J]. Proceedings of the National Academy of Sciences, 2014,111(51):18273-18278. [5] Ettwig K F, Butler M K, Le Paslier D, et al. Nitrite-driven anaerobic methane oxidation by oxygenic bacteria[J]. Nature, 2010,464(7288):543-548. [6] Haroon M F, Hu S, Shi Y, et al. Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage[J]. Nature, 2013,500(7464):567-570. [7] Hu S, Zeng R J, Keller J, et al. Effect of nitrate and nitrite on the selection of microorganisms in the denitrifying anaerobic methane oxidation process[J]. Environmental Microbiology Reports, 2011, 3(3):315-319. [8] Meng H, Zhang X, Zhou Z, et al. Simultaneous occurrence and analysis of both anammox and n-damo bacteria in five full-scale wastewater treatment plants[J]. International Biodeterioration & Biodegradation, 2021,156:105112. [9] Wang Y F, Dick R P, Lorenz N, et al. Interactions and responses of n-damo archaea, n-damo bacteria and anammox bacteria to various electron acceptors in natural and constructed wetland sediments[J]. International Biodeterioration & Biodegradation, 2019,144:104749. [10] Van Kessel M A, Stultiens K, Slegers M F, et al. Current perspectives on the application of N-damo and anammox in wastewater treatment[J]. Current Opinion in Biotechnology, 2018,50:222-227. [11] Kartal B, Tan N C, Van de Biezen E, et al. Effect of nitric oxide on anammox bacteria[J]. Applied and Environmental Microbiology, 2010,76(18):6304-6306. [12] Kuenen J G. Anammox bacteria:from discovery to application[J]. Nature Reviews Microbiology, 2008,6(4):320-326. [13] Suenaga T, Ota T, Oba K, et al. Combination of 15N tracer and microbial analyses discloses N2O sink potential of the anammox community[J]. Environmental Science & Technology, 2021,55(13):9231-9242. [14] Ettwig K F, Alen T, Schoonen K T, et al. Enrichment and molecular detection of denitrifying methanotrophic bacteria of the NC10 phylum[J]. Applied and Environmental Microbiology, 2009,75(11):3656- 3662. [15] Ettwig K F, Shima S, Schoonen K T, et al. Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea[J]. Environmental Microbiology, 2008,10(11):3164-3173. [16] Hu Z, Zhang J, Li S, et al. Effect of anoxic/aerobic phase fraction on N2O emission in a sequencing batch reactor under low temperature[J]. Bioresource Technology, 2011,102(9):5486-5491. [17] 吕娇,楼菊青,徐帆.反硝化型甲烷厌氧氧化(DAMO)系统pH值耦合模型研究[J]. 中国环境科学, 2022,42(2):612-619. Lv J, Lou J Q, Xu F. Study on pH coupling model of denitrification methane anaerobic oxidation (DAMO) system[J] China Environmental Science, 2022,42(2):612-619. [18] Raghoebarsing A A, Pol A, Schoonen K T, et al. A microbial consortium couples anaerobic methane oxidation to denitrification[J]. Nature, 2006,440(7086):918-921. [19] He Z, Geng S, Pan Y, et al. Improvement of the trace metal composition of medium for nitrite-dependent anaerobic methane oxidation bacteria:Iron (II) and copper (II) make a difference[J]. Water Research, 2015,85:235-243. [20] Fu L, Ding J, Lu Y Z, et al. Nitrogen source effects on the denitrifying anaerobic methane oxidation culture and anaerobic ammonium oxidation bacteria enrichment process[J]. Applied Microbiology and Biotechnology, 2017,101:3895-3906. [21] Frølund B, Palmgren R, Keiding K, et al. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research, 1996,30(8):1749-1758. [22] Ogata H, Goto S, Sato K, et al. KEGG:Kyoto encyclopedia of genes and genomes[J]. Nucleic Acids Research, 1999,27(1):29-34. [23] Zheng J, Doskey P V. Modeling nitrous oxide production and reduction in soil through explicit representation of denitrification enzyme kinetics[J]. Environmental Science & Technology, 2015, 49(4):2132-2139. [24] Li W, Lu P, Zhang L, et al. Long-term performance of denitrifying anaerobic methane oxidation under stepwise cooling and ambient temperature conditions[J]. Science of the Total Environment, 2020, 713:136739. [25] Tomaszewski M, Cema G, Ziembińska-Buczyńska A. Influence of temperature and pH on the anammox process:a review and meta-analysis[J]. Chemosphere, 2017,182:203-214. [26] 孙艳波,周少奇,李伙生,等.氮素负荷及高温冲击对UASB- ANAMMOX反应器的运行影响[J]. 化工进展, 2009,28(9):1672- 1676. Sun Y B, Zhou S Q, Li H S, et al. The effect of nitrogen loading and high temperature shock on the operation of UASB-ANAMMOX reactor[J]. Progress in Chemical Industry, 2009,28(9):1672-1676. [27] Lin X, Wang Y, Ma X, et al. Evidence of differential adaptation to decreased temperature by anammox bacteria[J]. Environmental Microbiology, 2018,20(10):3514-3528. [28] Ding Z W, Ding J, Fu L, et al. Simultaneous enrichment of denitrifying methanotrophs and anammox bacteria[J]. Applied Microbiology and Biotechnology, 2014,98:10211-10221. [29] Luesken F A, Alen T A, Biezen E, et al. Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge[J]. Applied Microbiology and Biotechnology, 2011, 92:845-854. [30] Zhu B, Sánchez J, Alen T A, et al. Combined anaerobic ammonium and methane oxidation for nitrogen and methane removal[J]. Biochemical Society Transactions, 2011,39(6):1822-1825. [31] Gü ven D, Dapena A, Kartal B, et al. Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria[J]. Applied and Environmental Microbiology, 2005,71(2):1066-1071. [32] Wang W, Wang T, Liu Q, et al. Biochar-mediated DNRA pathway of anammox bacteria under varying COD/N ratios[J]. Water Research, 2022,212:118100. [33] Glodowska M, Ma Y, Smith G, et al. Nitrate leaching and its implication for Fe and As mobility in a Southeast Asian aquifer[J]. Fems Microbiology Ecology, 2023,99(4):1-14. [34] Sun Y, De Vos P, Heylen K. Nitrous oxide emission by the non- denitrifying, nitrate ammonifier Bacillus licheniformis[J]. BMC Genomics, 2016,17(1):1-11. [35] Pereira T D S, Dos Santos C E D, Lu X, et al. Effect of operating conditions on N2O production in an anammox sequencing batch reactor containing granular sludge[J]. Water Science and Technology, 2019,80(1):37-47. [36] Caranto J D, Vilbert A C, Lancaster K M. Nitrosomonas europaea cytochrome P460 is a direct link between nitrification and nitrous oxide emission[J]. Proceedings of the National Academy of Sciences, 2016,113(51):14704-14709. [37] Pan Y, Ni B J, Bond P L, et al. Electron competition among nitrogen oxides reduction during methanol-utilizing denitrification in wastewater treatment[J]. Water Research, 2013,47(10):3273-3281. [38] Zhou Y, Oehmen A, Lim M, et al. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants[J]. Water Research, 2011, 45(15):4672-4682. [39] Kartal B, Kuypers M M, Lavik G, et al. Anammox bacteria disguised as denitrifiers:nitrate reduction to dinitrogen gas via nitrite and ammonium[J]. Environmental Microbiology, 2007,9(3):635-642. [40] Peng H, Guo J, Li H, et al. Granulation and response of anaerobic granular sludge to allicin stress while treating allicin-containing wastewater[J]. Biochemical Engineering Journal, 2021,169:107971. [41] Yuan C, Sun F, Zhang J, et al. Low-temperature-resistance granulation of activated sludge and the microbial responses to the granular structural stabilization[J]. Chemosphere, 2023,311:137146. [42] Li J, Yao Y, Shi Y, et al. FeCl4mediated inhibition and toxicity during anaerobic digestion:Dose-response kinetics, biochar-dependent detoxification and microbial resistance[J]. Water Research, 2022,210:117969. [43] He C S, He P P, Yang H Y, et al. Impact of zero-valent iron nanoparticles on the activity of anaerobic granular sludge:from macroscopic to microcosmic investigation[J]. Water Research, 2017, 127:32-40. [44] Sabba F, Terada A, Wells G, et al. Nitrous oxide emissions from biofilm processes for wastewater treatment[J]. Applied Microbiology and Biotechnology, 2018,102:9815-9829. [45] Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation− emission matrix regional integration to quantify spectra for dissolved organic matter[J]. Environmental Science & Technology, 2003,37(24):5701-5710. [46] Sheng G P, Yu H Q. Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J]. Water Research, 2006,40(6):1233-1239. [47] Wei D, Dong H, Wu N, et al. A fluorescence approach to assess the production of soluble microbial products from aerobic granular sludge under the stress of 2, 4-dichlorophenol[J]. Scientific Reports, 2016, 6(1):1-7. [48] Wang Z P, Zhang T. Characterization of soluble microbial products (SMP) under stressful conditions[J]. Water Research, 2010,44(18):5499-5509. [49] Ishii S K, Boyer T H. Behavior of reoccurring PARAFAC components in fluorescent dissolved organic matter in natural and engineered systems:a critical review[J]. Environmental Science & Technology, 2012,46(4):2006-2017. [50] Li W T, Chen S Y, Xu Z X, et al. Characterization of dissolved organic matter in municipal wastewater using fluorescence PARAFAC analysis and chromatography multi-excitation/emission scan:A comparative study[J]. Environmental Science & Technology, 2014,48(5):2603- 2609. [51] Amaral V, Ortega T, Romera-Castillo C, et al. Linkages between greenhouse gases (CO2, CH4, and N2O) and dissolved organic matter composition in a shallow estuary[J]. Science of the Total Environment, 2021,788:147863. [52] Ding H, Hu Q, Cai M, et al. Effect of dissolved organic matter (DOM) on greenhouse gas emissions in rice varieties[J]. Agriculture, Ecosystems & Environment, 2022,330:107870. [53] Long Y, Liu C, Lin H, et al. Vertical and horizontal distribution of sediment nitrite-dependent methane-oxidizing organisms in a mesotrophic freshwater reservoir[J]. Canadian Journal of Microbiology, 2017,63(6):525-534. [54] Ahn J, Kim W S, Park J B, et al. Temporal changes of geochemistry and microbial community in low and intermediate level waste (LILW) repository, South Korea[J]. Annals of Nuclear Energy, 2019,128:309-317. [55] Chakraborty A, Roden E E, Schieber J, et al. Enhanced growth of Acidovorax sp. strain 2AN during nitrate-dependent Fe (II) oxidation in batch and continuous-flow systems[J]. Applied and Environmental Microbiology, 2011,77(24):8548-8556. [56] Mao Y, Xia Y, Zhang T. Characterization of Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial communities by using high-throughput sequencing[J]. Bioresource Technology, 2013,128:703-710. [57] Tanikawa D, Sonaka H, Kadotani M, et al. Estimation of microbial community for denitrification in the down-flow hanging sponge (DHS) reactor[J]. International Biodeterioration & Biodegradation, 2020, 153:105022. [58] Zhao Y, Zeng D, Wu G. Efficient nitrous oxide production and metagenomics-based analysis of microbial communities in denitrifying systems acclimated with different electron acceptors[J]. International Biodeterioration & Biodegradation, 2019,138:92-98. [59] Pandey C, Kumar U, Kaviraj M, et al. DNRA:A short-circuit in biological N-cycling to conserve nitrogen in terrestrial ecosystems[J]. Science of the Total Environment, 2020,738:139710. [60] Lai T V, Ryder M H, Rathjen J R, et al. Dissimilatory nitrate reduction to ammonium increased with rising temperature[J]. Biology and Fertility of Soils, 2021,57:363-372. [61] Xu X, Liu X, Li Y, et al. High temperatures inhibited the growth of soil bacteria and archaea but not that of fungi and altered nitrous oxide production mechanisms from different nitrogen sources in an acidic soil[J]. Soil Biology and Biochemistry, 2017,107:168-179. [62] Ma J, Yang Q, Wang S, et al. Effect of free nitrous acid as inhibitors on nitrate reduction by a biological nutrient removal sludge[J]. Journal of Hazardous Materials, 2010,175(1-3):518-523. [63] Warneke S, Schipper L A, Matiasek M G, et al. Nitrate removal, communities of denitrifiers and adverse effects in different carbon substrates for use in denitrification beds[J]. Water Research, 2011, 45(17):5463-5475. [64] Qian J, Zhang M, Pei X, et al. A novel integrated thiosulfate-driven denitritation (TDD) and anaerobic ammonia oxidation (anammox) process for biological nitrogen removal[J]. Biochemical Engineering Journal, 2018,139:68-73. [65] Nie W B, Ding J, Xie G J, et al. Anaerobic oxidation of methane coupled with dissimilatory nitrate reduction to ammonium fuels anaerobic ammonium oxidation[J]. Environmental Science & Technology, 2020,55(2):1197-1208. |
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