水合氧化物对抗生素抗性基因水平转移的影响

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Abstract This study explored the effects of hydrous oxides of aluminum and hydrous oxides of manganese on the horizontal transfer of ampicillin resistance genes carried by pUC19 plasmid to recipient E. coli cells, respectively. In the concentration range of 2.5~200mg/L, hydrous oxides significantly inhibited the horizontal transfer of antibiotic resistance genes (ARGs). As compared to the control group, inhibition rates of hydrous oxides of aluminum and hydrous oxides of manganese on transformation frequencies were up to 79.44% and 57.64%, respectively. The Al (III) released from hydrous oxides of aluminum are one of the reasons for the influenced ARG transformation, and the release of cation from hydrous oxides of manganese was negligible. In addition, hydrous oxides of aluminum can form large agglomerates with pUC19 plasmids by binding to their phosphate groups and bases of adenine, thymine, and guanine, thus hindering the entrance of ARGs to the cells. For hydrous oxides of manganese, they significantly decreased the membrane permeability of the recipient cells and also formed large-sized complexes with plasmids, which might be the mechanisms accounting for the inhibited ARG transfer.

Key words： soil hydrous oxides antibiotic resistance genes horizontal gene transfer

Received: 30 August 2023

PACS:

X53

Corresponding Authors: 胡小婕,副教授,huxiaojie@njau.edu.cn E-mail: huxiaojie@njau.edu.cn

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	LI Xiao-jia
	LIU Si
	WANG Ting-ting
	QIN Chao
	HU Xiao-jie
	GAO Yan-zheng

Cite this article:

LI Xiao-jia,LIU Si,WANG Ting-ting等. Influence of hydrous oxides on the horizontal transfer of antibiotic resistance genes[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1622-1629.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I3/1622

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