互花米草入侵对闽江口湿地土壤氨氧化微生物群落的影响

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Abstract To explore the differences in community structure and diversity of ammonia-oxidizing archaea (AOA) and ammonia- oxidizing bacteria (AOB) in marsh soils at different invasion stages of Spartina alterniflora, the mudflat (BF, before invasion) and the S. alterniflora marsh after seaward invasion for 1~2 years (SA M) and 6~7 years (SAM) in Shanyutan of the Min River estuary were investigated by the high-throughput sequencing method. Results showed that, as affected by S. alterniflora invasion, the richness and diversity (represented by OTUs, Chao1index and Shannon index) of AOA in marsh soils were significantly higher than those of AOB. The seaward invasion of S. alterniflora reduced the richness of AOA (represented by OTUs and Chao1index) and the diversity of AOB (represented by Shannon index), but increased the diversity of AOA and the richness of AOB in marsh soils. The AOA in soils of different invasion stages was predominated by Thaumarchaeota. Differently, the AOB in soils of BF and SAM were predominated by Crenarchaeota (>90%), while those of SAM was predominated by Pseudomonadota. Nitrosopumilus was the dominant bacterial genus in AOA and its relative abundance in soils of different invasion stages exceeded 98%. By comparison the unclassified bacterial genera were the dominant bacterial genus in AOB and its relative abundance in soils at different invasion stages exceeded 90%. The seaward invasion of S. alterniflora increased the spatial heterogeneity of microbial community composition among samples in marsh soils (especially AOB), which was primarily due to the increased spatial heterogeneity of environmental factors caused by strong siltation of S. alterniflora. This study found that the AOA predominated the process of ammonia oxidation in soils of different seaward invasion stages of S. alterniflora. The seaward invasion of S. alterniflora altered the physio-chemical properties (e.g., pH and EC) and N nutrient conditions in marsh soils, which greatly influenced the structure and diversity of ammonia-oxidizing microbial communities.

Key words： ammonia-oxidizing microorganisms high-throughput sequencing community structure Spartina alterniflora Min River estuary

Received: 09 September 2023

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X171

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	CHEN Bing-bing
	SUN Zhi-gao
	WU Hui-hui
	WANG Xiao-ying
	MAO Li
	LI Yan-zhe

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CHEN Bing-bing,SUN Zhi-gao,WU Hui-hui等. Effects of Spartina alterniflora invasion on ammonia-oxidizing microbial communities in marsh soils of the Min River estuary[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(4): 2309-2319.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I4/2309

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