|
|
|
| Achieving stable partial nitrification by combining heat-shock sludge and intermittent gradient aeration |
| LI Dong1, ZHANG Jing-zhao1, ZENG Fan-xu1, LI Zhu1, ZHANG Jie1,2 |
1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
|
|
|
|
Abstract A novel strategy combining heat shock and intermittent gradient aeration was proposed to achieve partial nitrification. The impact of the combined strategy on nitrifying bacteria was explored in a 120-day experiment. Furthermore, the long-term stability of partial nitrification under the combined strategy was optimized by adjusting the sludge discharge ratio as the primary variable. The results demonstrated that intermittent gradient aeration (DO: 2.0mg/L// DO: 1.2mg/L// DO: 0.5mg/L) could rapidly restore the activity of ammonia-oxidizing bacteria impaired by thermal treatment without external carbon source added, and NAR achieved over 80% in 5days. During the stabilization phase, SAOR and SNPR were 30.12 and 6.69mg N/(gVSS⋅h), respectively. And the effluent ammonia concentration was below 0.1mg/L with nitrite and nitrate concentration of 53.42 and 8.01mg/L. Further analysis showed that the NAR remained at 88.96% when the sludge discharge ratio was set at 1%, while it tended to decrease with the discharge ratio of 0.5% or another value of 1.5%.
|
|
Received: 25 November 2023
|
|
|
|
|
|
[1] 杨庆,杨玉兵,杨忠启,等.溶解氧对短程硝化稳定性及功能菌群的影响[J]. 中国环境科学, 2018,38(9):3328-3334. Yang Q, Yang Y B, Yang Z Q, et al. Effect of dissolved oxygen on the stability and functional microbial communities of the partial nitrification [J]. China Environmental Science, 2018,38(9):3328-3334.
[2] Zaman M, Kim M, Nakhla G. Simultaneous partial nitrification and denitrifying phosphorus removal (PNDPR) in a sequencing batch reactor process operated at low DO and high SRT for carbon and energy reduction [J]. Chemical Engineering Journal, 2021,425: 131881.
[3] 陈佩文,朱易春.超声对Cd冲击后短程硝化污泥活性的影响[J]. 江西冶金, 2022,42(6):101-107. Chen P W, Zhu Y C. Effect of ultrasound on partial nitrification sludge activity after cadmium shock [J]. Jiangxi Metallurgy, 2022, 42(6):101-107.
[4] Liu C, Wang Y, Chen G, et al. A novel stable nitritation process: Treating sludge by alternating free nitrous acid/heat shock [J]. Bioresource Technology, 2022,347:126753.
[5] Choi D, Cho K, Hwang K, et al. Achieving stable nitrogen removal performance of mainstream PN-ANAMMOX by combining high- temperature shock for selective recovery of AOB activity [J]. Science of The Total Environment, 2021,794:148582.
[6] Chen J, Zhang S, Han X, et al. Nitritation of real sewage: start-up and maintenance by the side-stream heat-shock treatment [J]. Water Science and Technology, 2019,79(4):753-758.
[7] Isaka K, Sumino T, Tsuneda S. Novel nitritation process using heat-shocked nitrifying bacteria entrapped in gel carriers [J]. Process Biochemistry, 2008,43(3):265-270.
[8] Yu L, Li R, Mo P, et al. Stable partial nitrification at low temperature via selective inactivation of enzymes by intermittent thermal treatment of thickened sludge [J]. Chemical Engineering Journal, 2021,418: 129471.
[9] Chen Y, Zhao Z, Liu H, et al. Achieving stable two-stage mainstream partial-nitrification/anammox (PN/A) operation via intermittent aeration [J]. Chemosphere, 2020,245:125650.
[10] Xu Z, Zhang L, Gao X, et al. Optimization of the intermittent aeration to improve the stability and flexibility of a mainstream hybrid partial nitrification-anammox system [J]. Chemosphere, 2020,261:127670.
[11] Cui B, Yang Q, Liu X, et al. The effect of dissolved oxygen concentration on long-term stability of partial nitrification process [J]. Journal of Environmental Sciences, 2020,90:343-351.
[12] Pedrouso A, Val Del Rio A, Morales N, et al. Mainstream anammox reactor performance treating municipal wastewater and batch study of temperature, pH and organic matter concentration cross-effects [J]. Process Safety and Environmental Protection, 2021,145:195-202.
[13] Gao R, Peng Y, Li J, et al. Nutrients removal from low C/N actual municipal wastewater by partial nitritation/anammox (PN/A) coupling with a step-feed anaerobic-anoxic-oxic (A/A/O) system [J]. Science of The Total Environment, 2021,799:149293.
[14] Ding J, Gong H, Wang S, et al. Performance of partial nitritation - microfiltration-anammox (PN-MF-A) process with enhanced system stability via in-between membrane filtration for sludge anaerobic reject water treatment [J]. Journal of environmental chemical engineering, 2021,9(5):106005.
[15] Wang J, Zhang Z, Qian F, et al. Rapid start-up of a nitritation granular reactor using activated sludge as inoculum at the influent organics/ammonium mass ratio of 2/1[J]. Bioresource Technology, 2018,256:170-177.
[16] 王增花,王博,宫小斐,等. FNA处理絮体污泥恢复城市污水PN/A工艺短程硝化[J]. 中国环境科学, 2019,39(8):3308-3315. Wang Z H, Wang B, Gong X F, et al. Restoration of nitritation of the sewage partial nitrification/anammox (PN/A) process using free nitrous acid to treat flocculent sludge [J]. China Environmental Science, 2019,39(8):3308-3315.
[17] 张杰,张泽文,李冬,等.不同污泥龄厌氧氨氧化菌的脱氮效能及其动力学特性[J]. 哈尔滨工业大学学报, 2018,50(8):1-7. Zhang J, Zhang Y W, Li D, et al. Nitrogen removal efficiency and kinetic characteristics of anammox bacteria at different sludge ages [J]. Journal of Harbin Institute of Technology, 2018,50(8):1-7.
[18] 施永生.亚硝酸型生物脱氮技术[J]. 给水排水, 2000,26(11):21-23. Shi Y S. Nitrous biological nitrogen removal technology [J]. Water & Wastewater Engineering, 2000,26(11):21-23.
[19] Degraaf A, Debruijn P, LA Robertson, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor [J]. Microbiology, 1996,142:2187-2196.
[20] Li X Y, Yang S F. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge [J]. Water Research, 2007,41(5):1022-1030.
[21] Yu G, He P, Shao L, et al. Toward understanding the mechanism of improving the production of volatile fatty acids from activated sludge at pH 10.0[J]. Water Research, 2008,42(18):4637-4644.
[22] 王琪,李冬,李鹏垚,等.厌/缺氧时间对好氧颗粒污泥同步硝化内源反硝化和除磷的影响[J]. 中国环境科学, 2022,42(9):4199-4206. Wang Q, Li D, Li P Y, et al. Effects of anaerobic/anoxic time on simultaneous nitrification-endogenous denitrification and phosphorous removal from aerobic granular sludge [J]. China Environmental Science, 2022,42(9):4199-4206.
[23] Li S, Duan H, Zhang Y, et al. Adaptation of nitrifying community in activated sludge to free ammonia inhibition and inactivation [J]. Science of The Total Environment, 2020,728:138713.
[24] Liu W, Yang Q, Ma B, et al. Rapid achievement of nitritation using aerobic starvation [J]. Environmental Science & Technology, 2017, 51(7):4001-4008.
[25] 李冬,刘志诚,徐贵达,等.间歇恒定/梯度曝气对SNAD工艺启动的影响[J]. 环境科学, 2019,40(12):5438-5445. Li D, Liu Z C, Xu G D, et al. Effects of differnet intermittent aeration strategies on the start-up of SNAD process [J]. Environmental Science, 2019,40(12):5438-5445.
[26] 沈佳鑫.序批式活性污泥反应器部分亚硝化影响因素的研究[D]. 上海:东华大学, 2022:25-44. Shen J X. Study on influencing factors of partial nitrification in sequencing batch activated sludge reactor [D]. Shang'hai: Dong'hua University, 2022:25-44.
[27] Torà J A, Lafuente J, Baeza J A, et al. Long-term starvation and subsequent reactivation of a high-rate partial nitrification activated sludge pilot plant [J]. Bioresource Technology, 2011,102(21):9870- 9875.
[28] 张广瑞,任彬,李海松.煤化工废水除氟及尾水脱氮除磷性能[J]. 中国环境科学, 2023,43(4):1655-1662. Zhang G R, Ren B, Li H S, et al. Defluorination of coal chemical wastewater and nitrogen and phosphorus removal performance of tailwater [J]. China Environmental Science, 2023,43(4):1655-1662.
[29] Wu C, Peng Y, Wang S, et al. Effect of sludge retention time on nitrite accumulation in real-time control biological nitrogen removal sequencing batch reactor [J]. Chinese Journal of Chemical Engineering, 2011,19(3):512-517.
[30] 张亮,李朝阳,彭永臻.城市污水PN/A工艺中NOB的控制策略研究进展[J]. 北京工业大学学报, 2022,48(4):421-429. Zhang L, Li C Y, Peng Y Z, et al. Recent progress of NOB control strategies in sewage PN/A Process [J]. Journal of Beijing University of Technology, 2022,48(4):421-429.
[31] 刘文如,阴方芳,丁玲玲,等.选择性排泥改善颗粒污泥亚硝化性能的研究[J]. 中国环境科学, 2014,34(2):396-402. Liu W R, Yin F F, Ding L L, et al. Improved nitritation performance by selective sludge discharge in aerobic granular sludge process. China Environmental Science, 2014,34(2):396-402.
[32] Zhu G, Wang S, Ma B, et al. Anammox granular sludge in low- ammonium sewage treatment: Not bigger size driving better performance [J]. Water Research, 2018,142:147-158.
[33] 李冬,毛中新,李明润,等.序批式与连续流交替运行的短程硝化启动研究[J]. 中国环境科学, 2023,43(7):3438-3446. Li D, Mao Z X, Li M R, et al. Study on the start-up characteristics of PN system running alternately with sequential batch and continuous flow [J]. China Environmental Science, 2023,43(7):3438-3446.
[34] 张静霄.微生物群落在膜法废水处理系统中的成膜行为和调控方法[D]. 合肥:中国科学技术大学, 2022:5-7. Zhang J X. The behaviors and regulation of microbial biofilm in membrane-based wastewater treatment system [D]. He'fei: University of Science and Technology of China, 2022:5-7.
[35] Liu T, Zheng X, Tang G, et al. Effects of temperature shocks on the formation and characteristics of soluble microbial products in an aerobic activated sludge system [J]. Process Safety and Environmental Protection, 2022,158:231-241.
[36] 刘小博.脱氮除磷系统中污泥沉降性能影响研究[D]. 西安:西安建筑科技大学, 2019:19-32. Liu X B. Study on effeet of sludge settleability in biological nitrogen and phosphorus removal system [D]. Xi'an:Xi'an University of Architecture and Technology, 2019:19-32. |
|
|
|
