低温对异养硝化菌<i>Acinetobacter</i> sp.生长代谢及群体感应影响研究

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Abstract To further explore the mechanism of nitrogen transformation and quorum sensing system of HN-AD bacteria under low temperature, there were at least three AHLs signaling molecules, including C₆-, C₈-, and C₁₀-HSL produced by the HN-AD bacterium JQ1004 based on the analysis of GC-MS. The concentration of AHLs showed a significant density dependence and continuously accumulated with the increase of biomass. When the temperature decreased from the optimal 30℃ to 10℃, C₆- and C₈-HSL showed a continuous decreasing trend due to the decrease of cell concentration, while the concentration of C₁₀-HSL increased first and then decreased, reaching a maximum value of 10.89nmol/L at 20℃, indicating that C₁₀-HSL played a crucial role in the bacterial adaptation to low-temperature environments. Further study found that adding 10nmol/L C₁₀-HSL at low temperature could significantly shorten the bacterial lag period and improved the nitrogen removal rate. The addition of C₁₀-HSL at 20℃ and 10℃ increased the expression of amoA by 35% and 22%, respectively, while the expression of napA increased by 14% and 9.4%. C₁₀-HSL enhanced the ability of quorum sensing of strains by increasing the receptor protein activity regulated by luxR. Adding C₁₀-HSL at low temperature could promote extracellular protein secretion, reduce polysaccharide content, improve biofilm activity, and strengthen its adaptability to harsh low-temperature environments.

Key words： heterotrophic nitrification aerobic denitrification quorum sensing low temperature AHLs

Received: 07 November 2023

PACS:

X703.5

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	Articles by authors
	WANG Yi-shu
	LI Yuan
	HOU Lian-gang
	CHEN Hui-xuan
	HOU Hui-min
	WANG Xiu-jie

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WANG Yi-shu,LI Yuan,HOU Lian-gang等. Effects of low temperature on the growth, metabolism, and quorum sensing of heterotrophic nitrifying bacteria Acinetobacter sp[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(7): 4063-4070.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I7/4063

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